Structural Dynamics at LCLS

LCLS 结构动力学

• 批准号: 10614406
• 负责人: Mark Hunter
• 金额: $ 27.69万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-01 至 2026-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10614406
• 关键词: Automobile Driving; Beds; Biochemical Reaction; Collaborations; Color; Communities; Complex; Consumption; Crystallization; Crystallography; Data; Data Collection; Databases; Development; Development Plans; Devices; Diffusion; Drug Design; Enzymatic Biochemistry; Exposure to; Feedback; Geometry; Goals; Lasers; Ligands; Liquid substance; Membrane; Membrane Proteins; Methods; Modeling; Particle Size; Pathway interactions; Pattern; Phase; Physiologic pulse; Preparation; Protein Array; Protein Dynamics; RNA; Radiation; Reaction; Research; Resolution; Roentgen Rays; Sampling; Scanning; Science; Services; Stream; Structure; Surface Tension; Suspensions; System; Technology; Temperature; Testing; Thinness; Time; Viscosity; Work; cryogenics; density; design; experimental study; fluid flow; metalloenzyme; movie; programs; screening; x-ray free-electron laser

ABSTRACT: TR&D-2 TR&D-2 tackles the challenges of sample delivery for data collection at the LCLS X-ray FEL. High intensity X-ray FEL beams used to probe sample structure will destroy the sample after exposure to a single X-ray pulse. As a result, experiments at LCLS require a sample delivery system that can replace the damaged sample between every X-ray pulse. Crystal injectors were the first crystal delivery method used for serial femtosecond diffraction and are commonly used at X-ray FEL facilities because they can efficiently deliver a large number of crystals, reduce background scattering and enable new classes of time resolved studies. Sample injectors produce a thin stream of crystals (or sample solution) by ejecting a suspension through a small orifice. X-ray pulses at a high repetition rate interrogate the crystal stream and a diffraction pattern is produced each time a crystal and an X-ray pulse coincide. While it is possible to collect serial data at cryogenic temperatures with rapid scanning fixed target systems, injectors can replenish room temperature samples at even faster rates. Furthermore, many biomedical problems require the unique features offered by sample injectors including methods that use rapid mixing to study biomolecular dynamics. TR&D-2 focuses on advancing injector-based sample delivery methods to enable research following the DBP themes, specifically, solving new structures of membrane proteins and other radiation sensitive biomolecules and enabling the study of protein dynamics including those of metalloenzymes. This will be achieved via the development of remote access capabilities for data collection for Lipidic Cubic Phase (LCP) and other viscous media samples, as well as through the thorough testing and optimization of rapid mixing injectors. TR&D-2 aims to maximize the efficiency in the use of the unique LCLS beam, promoting greater access to biomedical community and new scientific discovery.

摘要：TR＆D-2 TR＆D-2解决了LCLS X射线FEL的数据收集样品输送的挑战。高的 用于探测样品结构的强度X射线FEL光束会在暴露后破坏样品 到一个X射线脉冲。结果，LCLS的实验需要样本输送系统 可以更换每个X射线脉冲之间的损坏样品。晶体喷油器是第一个 用于连续飞秒衍射的晶体输送方法，通常在X射线上使用 FEL设施是因为它们可以有效地提供大量晶体，请降低背景 散射并实现新的时间解决研究。样品喷油器产生薄的 晶体流（或样品溶液）通过通过小孔口弹出悬浮液。 X射线 高度重复速率的脉冲询问晶体流，并产生衍射模式 每次晶体和X射线脉冲一致。虽然可以在 喷射器可以通过快速扫描固定目标系统进行快速温度，可以补充房间 温度样本以更快的速度。此外，许多生物医学问题需要 样品喷油器提供的独特功能，包括使用快速混合进行研究的方法 生物分子动力学。 TR＆D-2着重于推进基于喷油器的样品输送方法，以实现研究 遵循DBP主题，特别是解决膜蛋白和其他的新结构 辐射敏感的生物分子并能够研究蛋白质动力学，包括 金属酶。这将通过开发数据的远程访问功能来实现 脂质立方相（LCP）和其他粘性培养基样品的收集 快速混合注射器的彻底测试和优化。 TR＆D-2旨在最大化 使用唯一LCLS梁的效率 社区和新科学发现。