Structural Dynamics at LCLS

LCLS 结构动力学

• 批准号: 10614406
• 负责人: Mark Hunter
• 金额: $ 27.69万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-01 至 2026-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10614406
• 关键词: Automobile Driving; Beds; Biochemical Reaction; Collaborations; Color; Communities; Complex; Consumption; Crystallization; Crystallography; Data; Data Collection; Databases; Development; Development Plans; Devices; Diffusion; Drug Design; Enzymatic Biochemistry; Exposure to; Feedback; Geometry; Goals; Lasers; Ligands; Liquid substance; Membrane; Membrane Proteins; Methods; Modeling; Particle Size; Pathway interactions; Pattern; Phase; Physiologic pulse; Preparation; Protein Array; Protein Dynamics; RNA; Radiation; Reaction; Research; Resolution; Roentgen Rays; Sampling; Scanning; Science; Services; Stream; Structure; Surface Tension; Suspensions; System; Technology; Temperature; Testing; Thinness; Time; Viscosity; Work; cryogenics; density; design; experimental study; fluid flow; metalloenzyme; movie; programs; screening; x-ray free-electron laser

ABSTRACT: TR&D-2 TR&D-2 tackles the challenges of sample delivery for data collection at the LCLS X-ray FEL. High intensity X-ray FEL beams used to probe sample structure will destroy the sample after exposure to a single X-ray pulse. As a result, experiments at LCLS require a sample delivery system that can replace the damaged sample between every X-ray pulse. Crystal injectors were the first crystal delivery method used for serial femtosecond diffraction and are commonly used at X-ray FEL facilities because they can efficiently deliver a large number of crystals, reduce background scattering and enable new classes of time resolved studies. Sample injectors produce a thin stream of crystals (or sample solution) by ejecting a suspension through a small orifice. X-ray pulses at a high repetition rate interrogate the crystal stream and a diffraction pattern is produced each time a crystal and an X-ray pulse coincide. While it is possible to collect serial data at cryogenic temperatures with rapid scanning fixed target systems, injectors can replenish room temperature samples at even faster rates. Furthermore, many biomedical problems require the unique features offered by sample injectors including methods that use rapid mixing to study biomolecular dynamics. TR&D-2 focuses on advancing injector-based sample delivery methods to enable research following the DBP themes, specifically, solving new structures of membrane proteins and other radiation sensitive biomolecules and enabling the study of protein dynamics including those of metalloenzymes. This will be achieved via the development of remote access capabilities for data collection for Lipidic Cubic Phase (LCP) and other viscous media samples, as well as through the thorough testing and optimization of rapid mixing injectors. TR&D-2 aims to maximize the efficiency in the use of the unique LCLS beam, promoting greater access to biomedical community and new scientific discovery.

摘要：R&D-2 TR&D-2解决了LCLS X射线自由电子激光数据采集的样品交付挑战。高 用于探测样品结构的强度X射线自由电子激光在曝光后会对样品造成破坏 到一个单一的X射线脉冲。因此，LCLS的实验需要一种样品输送系统，该系统 可以在每一次X射线脉冲之间更换损坏的样品。水晶注射器是第一个 用于连续飞秒衍射的晶体递送方法，在X射线中常用 FEL设施，因为它们可以高效地输送大量晶体，减少本底 散布和启用新的类别的时间分辨研究。样品注射器产生一种稀薄的 通过小孔喷射悬浮液形成晶体(或样品溶液)流。X射线 高重复频率的脉冲询问晶流并产生衍射图 每次晶体和X射线脉冲重合时。虽然可以在以下位置收集串行数据 低温快速扫描固定靶标系统，注射器可补充空间 温度采样的速度甚至更快。此外，许多生物医学问题需要 样品注射器提供的独特功能包括使用快速混合进行研究的方法 生物分子动力学。 R&D-2专注于先进的基于注射器的样品输送方法，以使研究成为可能 遵循DBP主题，特别是解决膜蛋白和其他 辐射敏感生物分子和蛋白质动力学研究，包括那些 金属酶。这将通过开发远程访问数据的能力来实现 收集脂肪立方相(LCP)和其他粘性介质样品，以及通过 对快速混合喷射器进行全面测试和优化。研发2号旨在最大限度地提高 提高使用独特的LCLS光束的效率，促进更多人获得生物医学 社区和新的科学发现。