Preventing outbreaks of foodborne pathogenic bacteria in edible sprouts via novel resistance-conferring seed treatments
通过新型抗性种子处理方法预防食用芽菜中食源性致病菌的爆发
基本信息
- 批准号:10589851
- 负责人:
- 金额:$ 99.13万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-04-01 至 2025-02-28
- 项目状态:未结题
- 来源:
- 关键词:AddressAffectAlfalfaAppearanceBacteriaBacterial CountsBiological SciencesCalciumCessation of lifeCloverConsumptionDevelopmentDiseaseDisease OutbreaksDoseEffectivenessEnteralEscherichia coli O157:H7Food SafetyFormulationGerminationGrowthHarvestHealth HazardsHospitalizationHumanHypochloriteIncidenceIndustryIndustry StandardInfectionInfiltrationLinkMeasuresMethodologyMethodsMicrobial BiofilmsNutrientPhasePlantsPowder dose formProcessProductionProliferatingPublic HealthRecommendationResearchResistanceRiskRoleSafetySalesSalmonellaSmell PerceptionSourceSystemTaste PerceptionTechnologyTestingTimeTissuesToxic effectToxicologyTreatment Efficacyantimicrobialcommercializationcompare effectivenessdefense responseefficacy testingenteric pathogenexperiencefoodbornefoodborne illnessfoodborne outbreakhazardhigh riskhuman pathogenimprovedinnovationlarge scale productionmicrobialmicrobiomemung beannew technologynovelpathogenpathogenic bacteriapreventprevent outbreaksscale upstandard caretreatment durationtreatment effect
项目摘要
Abstract
Ascribe Bioscience is developing a novel technology to prevent foodborne outbreaks caused by the
consumption of edible sprouts. Sprouts are highly vulnerable to infection with human enteric bacterial
pathogens at the seed stage. These pathogens then grow exponentially in the warm and humid conditions used
for sprouting. Even low initial bacterial counts on seeds can result in unsafe microbial loads by the end of sprout
production and processing. Moreover, the internalization of contaminating bacteria into seed tissues
contaminates the sprout tissues from inside, rendering the application of antimicrobials largely ineffective.
Sprouts have emerged as a significant source of foodborne illness and have become a public health hazard.
Between 1996 and 2017, the U.S. experienced 58 foodborne illness outbreaks associated with sprouts,
encompassing at least 1,953 illnesses, 212 hospitalizations, and 5 deaths. According to the FDA, no single
treatment so far has been shown to eliminate pathogens on seeds or sprouts that cause foodborne
illness. Ascribe Bioscience will use a natural, microbiome-derived molecule that activates and/or prime plant
defenses at the seed stage, thereby conferring to sprouts an enhanced resistance against contamination by
disease-causing bacterial pathogens. Ascribe's proposed treatment technology has the potential to
dramatically improve the safety of edible sprouts by addressing both external and internal bacterial
contamination. In Phase I, Ascribe has established the feasibility of the technology for the prevention of outbreaks
related to human consumption of sprouts by demonstrating that treatment with our molecule can prevent
Salmonella growth during the sprouting process in alfalfa. In Phase II, we will finalize the sprout seed treatment
formulation and expand testing to a broader spectrum of sprout-pathogen systems. To support
commercialization, we will perform toxicity and residue testing for regulatory approval, and refine synthesis
methodologies to enable large-scale production of the active ingredient. The specific aims of this Phase II project
are 1) Refine treatment formulation, including optimized formulation development, determination of the minimum
effective dose and treatment duration, comparison of the effectiveness of the formulation versus the sprout
industry-standard treatments, and measuring the effects of the formulation on germination, growth and sprout
quality; 2) Test the efficacy of treatments against other illness-causing pathogens on a variety of sprout types,
and evaluate it's role in altering bacterial abilities to form biofilm; 3) Demonstrate safety via toxicology studies
and residue testing to support EPA approval; 4) Develop methods to scale the synthesis of active ingredient to
pilot scale, and 5) Investigate the ability of the molecule to provide post-harvest protection to leafy greens. The
proposed research is expected to yield commercial products that will prevent or dramatically reduce the
incidence of sprout-related foodborne outbreaks.
摘要
归因于生物科学公司正在开发一种新技术,以防止由
食用豆芽。芽菜极易受人类肠道细菌感染。
种子阶段的病原体。然后,这些病原体在使用的温暖潮湿的条件下呈指数级增长
用来发芽。即使种子上的初始细菌数量很低,也会在萌芽结束时导致不安全的微生物负载
生产加工。此外,污染细菌内化到种子组织中
从内部污染发芽组织,使抗菌剂的应用在很大程度上无效。
芽菜已成为食源性疾病的一个重要来源,并已成为公共卫生危害。
1996至2017年间,美国经历了58起与豆芽有关的食源性疾病疫情,
包括至少1,953例疾病,212例住院治疗,5例死亡。根据FDA的说法,没有单一的
到目前为止,治疗方法已经被证明可以消除种子或芽上引起食源性疾病的病原体。
生病了。Ascribe Bioscience将使用一种来自微生物组的天然分子来激活和/或启动植物
在种子阶段进行防御,从而增强对污染的抵抗力
致病细菌病原体。Ascribe提出的治疗技术有可能
通过解决外部和内部细菌问题,极大地提高食用豆芽的安全性
污染。在第一阶段,Ascribe已经确定了该技术预防疫情的可行性
通过证明用我们的分子治疗可以防止人类食用豆芽
紫花苜蓿发芽过程中沙门氏菌的生长。在第二阶段,我们将最终确定萌芽种子处理
配方,并将测试扩大到更广泛的芽苗-病原菌系统。支持
商业化,我们将进行毒性和残留测试以获得监管部门的批准,并改进合成
使有效成分能够大规模生产的方法。二期工程的具体目标
1)精制处理配方,包括优化配方开发、确定最低用量
有效剂量和治疗时间,配方与萌芽的有效性比较
行业标准处理,并测量配方对种子萌发、生长和萌发的影响
质量;2)对各种萌芽类型的其他致病病原体的治疗效果进行测试,
评价其在改变细菌形成生物被膜能力方面的作用;3)通过毒理学研究证明其安全性
和残留物测试,以支持EPA的批准;4)开发方法,将活性成分的合成规模扩大到
中试规模,以及5)调查该分子为绿叶蔬菜提供收获后保护的能力。这个
预计拟议的研究将产生商业产品,这些产品将防止或显著减少
与芽苗相关的食源性暴发的发生率。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
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Murli Manohar其他文献
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{{ truncateString('Murli Manohar', 18)}}的其他基金
Using novel pre-harvest, resistance-conferring treatments to prevent foodborne pathogenic outbreaks in produce
使用新颖的收获前赋予抗性的处理方法来预防农产品中食源性致病菌的爆发
- 批准号:
10546010 - 财政年份:2022
- 资助金额:
$ 99.13万 - 项目类别:
Preventing outbreaks of foodborne pathogenic bacteria in edible sprouts via novel resistance-conferring seed treatments
通过新型抗性种子处理方法预防食用芽菜中食源性致病菌的爆发
- 批准号:
10384281 - 财政年份:2020
- 资助金额:
$ 99.13万 - 项目类别:
Preventing outbreaks of foodborne pathogenic bacteria in edible sprouts via novel resistance-conferring seed treatments
通过新型抗性种子处理方法预防食用芽菜中食源性致病菌的爆发
- 批准号:
10009714 - 财政年份:2020
- 资助金额:
$ 99.13万 - 项目类别:
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