Programmable evolution of optogenetic systems

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Project Summary: This project is specifically tailored for the candidate, Nhu Dao, to conduct biomedical research. Nhu’s research involves evolving novel proteins that can be photo-cleaved using light, allowing for uses in biological imaging, studying cellular and developmental biology, optically controlled 3D printing, and more. Using a system that enables a gene to continuously evolve, only cells containing a gene that responds to specific wavelengths (colors) of light will grow. This process can rapidly evolve this new class of proteins at an unprecedented pace. Proteins resulting from this evolution will be characterized using techniques from an undergraduate “Biochemical Techniques” course previously taught by the PI. The project uses hardware from the parent grant with minor modifications, making the timing and feasibility ideal for undergraduate research. In addition to the research training experience, the candidate will undergo extensive training in ethics, project management, learning/teaching-related skills, writing, presentation skills, interpersonal skills, and more. This training will initially take place in a “boot camp” setting and then be expanded upon throughout the year. Finally, this training and research will culminate in writing about the research in manuscript form and in a poster that will be presented in several poster symposia. Combined, this experience will boost the candidate’s career, preparing her for success in biomedical research in a 4-year program, graduate school, and beyond.