Core B: Functional Assessment & Instrumentation

抽象--科学的核心功能 科学核心B--“功能评估和器械核心”的首要目标是优化 用于心脏自主神经和心脏功能的高分辨率实时同步测量的生物电子技术 电生理参数。核心B将支持5项主要努力，这些努力是实现目标的基础 项目1至3：心脏EP标测：来自心肌内多极的数据分析进展 电极，再加上薄膜二维适形阵列的部署到心外膜，将定义电 跨缺血心脏交界区的异质性。互补的体外研究可以是 在我们的人体心脏/朗宁多夫准备中完成，使用电或光学EP标测。 神经化学检测：自主评估将包括实时测量局部心脏 神经递质释放谱，利用电化学循环伏安法(儿茶酚胺)和电容法 免疫探针(神经肽)。这样的神经化学测量同样可以从血管中获得 用于测量经心脏生物标记物释放情况的隔室。遥测：心脏连续监测 清醒猪受试者的心率、体温和活动度。慢性迷走神经刺激：使用FDA 批准的植入式迷走神经刺激设备，动物可以滴定到治疗水平的VNS和 在整个治疗阶段保持这种状态。心肌梗死的形成：加州大学洛杉矶分校 卓越神经心脏病学研究计划在创造慢性猪方面有十多年的经验 脑梗塞模型。这一模型已被广泛描述，并被证明可与患者的脑梗塞相媲美。 同时具有缺血性心脏病的电生理和组织病理学特征(1)。重要的是 该模型在星状神经节、心脏内脏神经的水平显示了显著的神经重构。 系统和心肌，使其适合拟议的PPG(2，3)。任务3至5由 加州大学洛杉矶分校卓越神经心脏病学研究项目拥有专门的大型动物无菌手术室 专为支持该PPG和2个专用PPG的所有器械和生存手术方面而设计 大型动物遥测室能够同时监测10只动物。

ABSTRACT – Scientific Core - Function The primary goal of scientific Core B – “Functional Assessment and Instrumentation core” - is to optimize bioelectronic technologies for high-resolution, real-time concurrent measurements of cardiac autonomic and electrophysiological parameters. Core B will support 5 major endeavors that a fundamental to completion of aims in Projects 1 to 3: Cardiac EP mapping: Advances in analytics for data derived from intra-myocardial multi-pole electrodes, coupled with the deployment of thin-film 2-D form-fit arrays to the epicardium, will define electrical heterogeneities across the border zone areas of the ischemic heart. Complementary in vitro studies can be accomplished in our human heart/Langendorff preparation utilizing either electrical or optical EP mapping. Neurochemical detection: Autonomic assessment will include real-time measurement of regional cardiac neurotransmitter release profiles, leveraging electrochemical cyclic voltammetry (catecholamine) and capacitive immunoprobe (neuropeptide). Such neurochemical measurements can likewise be obtained from vascular compartment to measure trans-cardiac biomarker release profiles. Telemetry: Continuous monitoring for heart rate, temperature and activity in conscious porcine subjects. Chronic Vagal Nerve stimulation: Using FDA approved implantable vagal nerve stimulation devices, animals can be titrated to therapeutic levels of VNS and maintained in that state throughout the treatment phase. Creation of myocardial infarcts: The UCLA Neurocardiology Research Program of Excellence has over a decade of experience in creating a chronic porcine infarct model. This model has been extensively characterized and shown to be comparable to infarcts in patients with ischemic heart disease in both electrophysiological and histopathological characteristics (1). Importantly, this model displays significant neural remodeling at the level of the stellate ganglia, intrinsic cardiac nervous system, and myocardium, making it suitable for the proposed PPG (2, 3). Tasks 3 to 5 are facilitated by the UCLA Neurocardiology Research Program of Excellence having a dedicated large animal aseptic surgical suite designed specifically to support all instrumentation and survival surgery aspects of this PPG and 2 dedicated large animal telemetry rooms capable of monitoring 10 animals at a time.