NABOR: A Sustainable, High-quality Neuroscience Antibody Open Resource

NABOR：可持续、高质量的神经科学抗体开放资源

• 批准号: 10627887
• 负责人: Melina Fan
• 金额: $ 179.26万
• 依托单位: ADDGENE, INC.
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-06-01 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10627887
• 关键词: Affinity; Aliquot; Antibodies; Antibody Formation; Award; Awareness; BRAIN initiative; Biological Assay; Brain; California; Catalogs; Collection; Communities; Computer software; Data; Deposition; Doctor of Philosophy; Education; Education and Outreach; Educational workshop; Future; Goals; Grant; Growth; Incentives; Infrastructure; Laboratories; Libraries; Maps; Massachusetts; Measures; Modeling; Molecular; Monoclonal Antibodies; Neurosciences; Neurosciences Research; Online Systems; Outcome; Performance; Plasmids; Production; Proteins; Protocols documentation; Publications; Quality Control; Reagent; Recombinant Antibody; Recombinants; Research Personnel; Resources; Scientist; Services; Technology; United States National Institutes of Health; Universities; Validation; Viral; Viral Vector; Work; crowdsourcing; data hub; data sharing; digital; education resources; empowerment; experience; nanobodies; neural circuit; outreach; sample collection; sharing platform; tool

PROJECT SUMMARY The goal of this project is to build an open-access recombinant antibody/affinity reagent resource for the neuroscience community called NABOR (Neuroscience AntiBody Open Resource). Reliable access to affinity reagents validated for use in diverse neuroscience research applications is crucial to accomplishing numerous goals of the BRAIN Initiative. Currently available affinity reagents from companies can be variable in quality and the molecular identity of the material is completely unknown to scientists. Scientists are now creating recombinant, plasmid-based affinity reagents such as recombinant monoclonal antibodies (R-mAbs), Single Chain Variable Fragments (ScFVs) and nanobodies (nAbs). Addgene, a nonprofit company, is a leader in plasmid storage, quality control, and distribution. Addgene will create NABOR as an open-access library of these plasmids and will provide them in ready-to-use protein format, along with protocols and educational guides. Aim 1 is to build a high-quality production and distribution pipeline for ready-to-use recombinant affinity reagents. Two leading BRAIN Initiative investigators, James Trimmer, Ph.D., of the University of California Davis, and Kwanghun Chung, Ph.D., of the Massachusetts Institute of Technology, will kick-start NABOR by contributing their extensive collections of neuroscience affinity reagents to this collection. Each lot will be subjected to rigorous quality control, initially in Dr. Trimmer’s laboratory and later at Addgene. Aim 2 is to build an open-access community resource platform for data sharing. This will include a web-based Data Hub to crowdsource reagent validation in a variety of applications. Aim 3 is to execute an education and outreach strategy that raises awareness of existing tools and solicits new tools. Addgene will share protocols and educational guides to make scientists successful when using the reagents and work directly with the community to create the NABOR catalog needed for neural circuit mapping and our understanding brain function. NABOR reagents and supporting information will be made available through Addgene’s existing channels for production, distribution and technical support. Successful completion of this project will result in enhanced visibility, access, and value for affinity reagents created by the BRAIN Initiative.

项目摘要 本项目的目标是建立开放获取的重组抗体/亲和试剂资源， NABOR（Neuroscience Antibody Open Resource）可靠访问affinity 经验证可用于各种神经科学研究应用的试剂对于实现许多神经科学研究应用至关重要。 BRAIN倡议的目标。目前可从公司获得的亲和试剂在质量上是可变的 而这种物质的分子身份对科学家来说是完全未知的。科学家们正在创造 重组的、基于质粒的亲和试剂，如重组单克隆抗体（R-mAbs）、单克隆抗体（mAb）和单克隆抗体（mAb）。 链可变片段（ScFV）和纳米抗体（nAb）。 Addgene是一家非营利性公司，是质粒储存、质量控制和分销领域的领导者。Addgene将 创建NABOR作为这些质粒的开放获取文库，并将以即用型蛋白质的形式提供它们 格式，沿着协议和教育指南。目标1：打造高品质的生产和分销平台 即用型重组亲和试剂的管道。两个领先的大脑倡议调查员，詹姆斯 Trimmer博士，加州大学戴维斯分校的教授，以及Kwanghun Chung博士，马萨诸塞州 技术研究所，将通过贡献他们广泛的神经科学亲和力的集合来启动NABOR 这一系列的试剂。每一批都将受到严格的质量控制，最初在特里默博士的 实验室，后来在Addgene。目标二是建立一个开放获取的社区数据资源平台 共享这将包括一个基于网络的数据中心，用于在各种应用中进行众包试剂验证。 目标3是执行一项教育和外联战略，提高对现有工具的认识， 工具. Addgene将分享协议和教育指南，以使科学家在使用时取得成功 试剂，并直接与社区合作，创建神经回路映射所需的NABOR目录 以及我们对大脑功能的理解。 NABOR试剂和支持信息将通过Addgene现有渠道提供， 生产、销售和技术支持。该项目的成功完成将提高 由BRAIN Initiative创建的亲和试剂的可见性、可访问性和价值。