NABOR: A Sustainable, High-quality Neuroscience Antibody Open Resource

NABOR：可持续、高质量的神经科学抗体开放资源

• 批准号: 10627887
• 负责人: Melina Fan
• 金额: $ 179.26万
• 依托单位: ADDGENE, INC.
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-06-01 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10627887
• 关键词: Affinity; Aliquot; Antibodies; Antibody Formation; Award; Awareness; BRAIN initiative; Biological Assay; Brain; California; Catalogs; Collection; Communities; Computer software; Data; Deposition; Doctor of Philosophy; Education; Education and Outreach; Educational workshop; Future; Goals; Grant; Growth; Incentives; Infrastructure; Laboratories; Libraries; Maps; Massachusetts; Measures; Modeling; Molecular; Monoclonal Antibodies; Neurosciences; Neurosciences Research; Online Systems; Outcome; Performance; Plasmids; Production; Proteins; Protocols documentation; Publications; Quality Control; Reagent; Recombinant Antibody; Recombinants; Research Personnel; Resources; Scientist; Services; Technology; United States National Institutes of Health; Universities; Validation; Viral; Viral Vector; Work; crowdsourcing; data hub; data sharing; digital; education resources; empowerment; experience; nanobodies; neural circuit; outreach; sample collection; sharing platform; tool

PROJECT SUMMARY The goal of this project is to build an open-access recombinant antibody/affinity reagent resource for the neuroscience community called NABOR (Neuroscience AntiBody Open Resource). Reliable access to affinity reagents validated for use in diverse neuroscience research applications is crucial to accomplishing numerous goals of the BRAIN Initiative. Currently available affinity reagents from companies can be variable in quality and the molecular identity of the material is completely unknown to scientists. Scientists are now creating recombinant, plasmid-based affinity reagents such as recombinant monoclonal antibodies (R-mAbs), Single Chain Variable Fragments (ScFVs) and nanobodies (nAbs). Addgene, a nonprofit company, is a leader in plasmid storage, quality control, and distribution. Addgene will create NABOR as an open-access library of these plasmids and will provide them in ready-to-use protein format, along with protocols and educational guides. Aim 1 is to build a high-quality production and distribution pipeline for ready-to-use recombinant affinity reagents. Two leading BRAIN Initiative investigators, James Trimmer, Ph.D., of the University of California Davis, and Kwanghun Chung, Ph.D., of the Massachusetts Institute of Technology, will kick-start NABOR by contributing their extensive collections of neuroscience affinity reagents to this collection. Each lot will be subjected to rigorous quality control, initially in Dr. Trimmer’s laboratory and later at Addgene. Aim 2 is to build an open-access community resource platform for data sharing. This will include a web-based Data Hub to crowdsource reagent validation in a variety of applications. Aim 3 is to execute an education and outreach strategy that raises awareness of existing tools and solicits new tools. Addgene will share protocols and educational guides to make scientists successful when using the reagents and work directly with the community to create the NABOR catalog needed for neural circuit mapping and our understanding brain function. NABOR reagents and supporting information will be made available through Addgene’s existing channels for production, distribution and technical support. Successful completion of this project will result in enhanced visibility, access, and value for affinity reagents created by the BRAIN Initiative.

项目总结 本项目的目标是建立一个开放获取的重组抗体/亲和试剂资源，用于 神经科学社区称为NABOR(神经科学抗体开放资源)。可靠访问亲和力 经过验证可用于各种神经科学研究应用的试剂对于实现 大脑计划的目标。目前公司提供的亲和试剂的质量可能会有所不同 而这种材料的分子身份对科学家来说是完全未知的。科学家们现在正在创造 重组、以质粒为基础的亲和试剂，如重组单抗(R-mAbs) 链可变片段(ScFv)和纳米小体(NaB)。 Addgene是一家非营利性公司，在质粒存储、质量控制和分配方面处于领先地位。阿德基尼将 创建NaBOR作为这些质粒的开放访问文库，并将以即用蛋白的形式提供它们 格式，以及协议和教育指南。目标一是打造高品质的生产和经销 即用型重组亲和试剂流水线。两位大脑计划的主要研究人员，詹姆斯 Trimmer博士，加州大学戴维斯分校博士；邝浑Chung博士，马萨诸塞州博士 将通过贡献他们广泛的神经科学亲和力来启动Nabor 这个收藏的试剂。每一批都将受到严格的质量控制，最初是在Trimmer博士的 实验室，后来在Addgene。目标2是建立一个开放获取的社区数据资源平台 分享。这将包括一个基于网络的数据中心，用于在各种应用程序中众包试剂验证。 目标3是执行一项教育和推广战略，以提高人们对现有工具的认识，并招募新的 工具。Addgene将分享协议和教育指南，使科学家在使用 试剂，并直接与社区合作，创建神经回路映射所需的Nabor目录 以及我们对大脑功能的理解。 NaBOR试剂和支持信息将通过Addgene现有的渠道提供 生产、分销和技术支持。该项目的成功完成将导致增强 大脑计划创造的亲和试剂的可见性、可及性和价值。