Opsins as a new class of evolutionarily conserved taste receptors: Diversity Supplement for Pre-Doctoral Training
视蛋白作为一类新的进化保守味觉受体:博士前培训的多样性补充
基本信息
- 批准号:10750801
- 负责人:
- 金额:$ 6.05万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2017
- 资助国家:美国
- 起止时间:2017-06-01 至 2027-05-31
- 项目状态:未结题
- 来源:
- 关键词:AddressAedesAmino AcidsAnimalsBehaviorBehavioral AssayBiological AssayBloodCRISPR/Cas technologyCatechinCellular biologyChemicalsClustered Regularly Interspaced Short Palindromic RepeatsCulicidaeDataDengueDetectionDevelopmentDietDiseaseDisease VectorsDrosophila genusElectrophysiology (science)EsthesiaEvolutionFamilyFemaleFlavonoidsFruitG-Protein-Coupled ReceptorsGeneticGoalsGrapefruitHealthHomologous GeneHumanImageImpairmentIn VitroInsectaLigand BindingLigandsLightMammalsModalityModelingMolecular GeneticsMotivationMusMutagenesisMutant Strains MiceMutationNeuronsNutrientNutritionalOpsinOrganPartner in relationshipPersonsPhotoreceptorsPlantsPoisonPopulationProductionPropertyProteinsReceptor ActivationReceptor CellRhodopsinRoleSpecificityTaste BudsTaste PerceptionTestingTongueTrainingTransgenic OrganismsVertebrate PhotoreceptorsVirusVisual SystemVitamin AWorkYellow FeverZIKAantagonistbehavior testchromophoredefined contributiondetectordietaryeggexperienceexperimental studyfascinatefeedingflygenetic manipulationhuman diseaseimprovedin vitro Assayin vivoinsect disease vectorinterestmalemembermutantpre-doctoralreceptorresponsesugartooltranscriptometranscriptome sequencingvector
项目摘要
Abstract
Opsins are classical G-protein coupled receptors, which for more than a century have been thought to function
exclusively as light detectors. However, using Drosophila, we have uncovered multiple, light-independent roles
for opsins including detection of bitter tastants. This discovery raises exciting questions, which we propose to
address here. Do opsins only respond to bitter compounds, or do they function more broadly in taste
sensation? Of particular interest, do opsins serve as taste receptors in other animals, including the mosquito,
Aedes (Ae.) aegypti? This vector spreads viruses that cause dengue, yellow fever, and other diseases that
afflict millions of people each year. Both male and female Aedes use taste to identify nectar and avoid toxic
chemicals, while females use many senses to identify people for blood meals. However, the receptors required
for mosquito taste are largely unknown. Another fascinating question is whether opsins serve as taste
receptors in mammals. To address these questions, we propose to employ an extensive suite of approaches
including electrophysiology, Ca2+ imaging, behavioral assays, cell biology, an in vitro receptor activation assay,
and state-of-the art molecular genetics. We will also bring to bear our expertise in Drosophila taste, opsins, and
our recent experience performing molecular genetics and behavior in Ae. aegypti and in the mouse. Aim 1 will
define the role of a Drosophila opsin in amino acid taste, which in flies is a poorly understood taste modality.
Attraction to amino acids increases if the flies are kept on an amino acid deficient diet. We outline experiments
to define the contribution of the opsin to this dynamic change in amino acid appeal. We will also test the
proposal that the opsin is activated directly by amino acids. Aim 2 will build on our preliminary data that two Ae.
aegypti opsins are expressed in the major taste organ, and are required for sensing a flavonoid. The goals of
this aim are to reveal the chemical specificity of these opsins in vitro and in vivo, determine whether they act
independently or together, and identify the gustatory receptor neurons in which these opsins function. Aim 3
will characterize the roles of opsins in mammalian taste. This aim is supported by preliminary data showing
that multiple mouse opsins are expressed in the mouse tongue, and are activated in vitro by bitter compounds,
including a flavonoid for which there is no known receptor. In addition, we present preliminary data that the
response to this flavonoid is impaired in the opsin mutant. We propose to test the hypothesis that this opsin
functions broadly in sensing flavonoids, identify the taste receptor cells that express this and the other
“gustatory opsins,” and perform behavioral tests with mutants to characterize the requirements for each opsin
for sensing bitter compounds. The results from this project would establish opsins as a new class of taste
receptor conserved from insects to mammals. We propose that defining opsins as the first bitter receptors in
mosquitoes offers the potential to harness this information to manipulate mosquito behavior and thereby
reduce their populations and thus insect-borne disease.
摘要
项目成果
期刊论文数量(7)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Mechanism for food texture preference based on grittiness.
- DOI:10.1016/j.cub.2021.02.007
- 发表时间:2021-05-10
- 期刊:
- 影响因子:0
- 作者:Li Q;Montell C
- 通讯作者:Montell C
Drosophila proboscis extension response and GCaMP imaging for assaying food appeal based on grittiness.
- DOI:10.1016/j.xpro.2022.101806
- 发表时间:2022-12-16
- 期刊:
- 影响因子:0
- 作者:Li, Qiaoran;Yang, Libo;Montell, Craig
- 通讯作者:Montell, Craig
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CRAIG MONTELL其他文献
CRAIG MONTELL的其他文献
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{{ truncateString('CRAIG MONTELL', 18)}}的其他基金
Opsins and TRP channels controlling sensation and behavior in Aedes aeygpti
视蛋白和 TRP 通道控制白纹伊蚊的感觉和行为
- 批准号:
10338805 - 财政年份:2021
- 资助金额:
$ 6.05万 - 项目类别:
Opsins and TRP channels controlling sensation and behavior in Aedes aeygpti
视蛋白和 TRP 通道控制白纹伊蚊的感觉和行为
- 批准号:
10493295 - 财政年份:2021
- 资助金额:
$ 6.05万 - 项目类别:
Opsins and TRP channels controlling sensation and behavior in Aedes aeygpti
视蛋白和 TRP 通道控制白纹伊蚊的感觉和行为
- 批准号:
10676854 - 财政年份:2021
- 资助金额:
$ 6.05万 - 项目类别:
Receptors and channels controlling sensation and behavior in Aedes aegypti
控制埃及伊蚊感觉和行为的受体和通道
- 批准号:
10207825 - 财政年份:2020
- 资助金额:
$ 6.05万 - 项目类别:
Non-classical roles for opsins in taste and smell
视蛋白在味觉和嗅觉中的非经典作用
- 批准号:
9363622 - 财政年份:2017
- 资助金额:
$ 6.05万 - 项目类别:
Opsins as a new class of evolutionarily conserved taste receptors
视蛋白作为一类新的进化上保守的味觉受体
- 批准号:
10657704 - 财政年份:2017
- 资助金额:
$ 6.05万 - 项目类别:
Creation of a new generation of transgenic mosquitoes to control infectious disease
创造新一代转基因蚊子来控制传染病
- 批准号:
9140088 - 财政年份:2015
- 资助金额:
$ 6.05万 - 项目类别:
TRPA1: a polymodal sensor for aversive stimuli
TRPA1:用于厌恶刺激的多模态传感器
- 批准号:
8294132 - 财政年份:2008
- 资助金额:
$ 6.05万 - 项目类别:
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