Molecular Genomics Core

项目总结-分子基因组学共享资源 南加州大学诺里斯综合癌症分子基因组学(MGC)共享资源(SR)的使命 中心(NCCC)是为行业标准和最先进的分子和基因组分析提供服务 以支持NCCC研究人员在基础、翻译、临床和人群方面的工作 科学。由NCCC管理部门监督，并在John Carpten博士和David Craig领导下 博士学位都是在当前周期中新招聘到NCCC的，MGC是行业的基础要素- 在大规模SNP基因分型、DNA甲基化阵列、 在体细胞、转录体和表观遗传学方面的综合基因组测序以及最新状态 ART单细胞和空间分子图谱。本周期的主要发展包括获得更大的产量- 规模排序，确保及早获得变革性新兴技术，并实施 生物信息学过程整合到其他SRS中。具体来说，MGC：1)增加了高通量测序 ARM采用先进的机器人技术和Illumina NovaSeq从头开始构建，以支持大型项目；2) 与行业合作伙伴建立了新的合作关系，以确保可用性和及早访问高级状态- 最先进的单细胞和空间分子图谱，包括10X基因组学、安捷伦和纳米串；以及3) 实施自动化API、生物信息学和数据处理，以与数据科学紧密协作 大规模Novaseq测序中的共享资源(DSC)。与NCCC战略计划保持一致，在 在下一个拨款周期，MGC计划进行全面的分子图谱分析，并与经过管理的患者数据相关联， 包括单细胞基因组学，用于筛查和残留疾病的液体活组织检查，以及成像质量细胞术 和空间转录以捕捉肿瘤和肿瘤微环境的相互作用应用于NCCC的多样化 病人群体。在目前的赠款期间(2015-2020年)，代表所有五个成员的122个NCCC成员 项目利用了MGC，直接结果是出版了233种出版物和5840万美元的直接成本赠款资金 使用率。MGC在下一个赠款周期第一年的预计年度预算为2,875,229美元，但 CCSG的要求是457,972美元。因此，MGC利用广泛的机构和充值支持 而且只从CCSG基金中寻求16%的份额。

PROJECT SUMMARY – Molecular Genomics Shared Resource The mission of the Molecular Genomics (MGC) Shared Resource (SR) of the USC Norris Comprehensive Cancer Center (NCCC) is to provide services for industry-standard and state-of-the-art molecular and genomic assays in support of NCCC investigators working across the continuum of basic, translational, clinical, and population sciences. Overseen by NCCC Administration and under the leadership of John Carpten PhD and David Craig PhD, both newly recruited to NCCC in the current cycle, the MGC serves as a foundational element with industry- standard technologies and field-leading expertise in large-scale SNP genotyping, DNA methylation arrays, integrated genomic sequencing at the somatic, transcriptomic, and epigenetic landscape as well as state-of-the- art single-cell and spatial molecular profiling. Major developments this cycle include access to larger production- scale sequencing, ensuring early access to transformative emerging technologies, and implementation of bioinformatic processes integration into other SRs. Specifically, MGC: 1) added a high-throughput sequencing arm built from the ground up with advanced robotics and Illumina NovaSeq to enable large-scale projects; 2) established new collaborations with industry partners to ensure availability and early access to advanced state- of-the-art single-cell and spatial molecular profiling, including 10X Genomics, Agilent, and Nanostring; and 3) implemented automated APIs, bioinformatics, and data processing for tight coordination with the Data Science Shared Resource (DSC) in large-scale Novaseq sequencing. In alignment with the NCCC Strategic Plan, in the next grant cycle, MGC plans to perform comprehensive molecular profiling with linkage to curated patient data, including single-cell genomics, liquid biopsies for screening and residual disease, and imaging mass cytometry and spatial transcriptomics to capture tumor and tumor microenvironment interactions applied to NCCC's diverse patient population. During the current grant period (2015-2020), 122 NCCC members representing all five programs utilized the MGC, resulting in 233 publications and $58.4M in direct cost grant funding as a direct result of usage. The anticipated annual budget of the MGC in the first year of the next grant cycle is $2,875,229, yet the CCSG request is $457,972. Accordingly, the MGC leverages extensive institutional and recharge support and seeks only 16% from CCSG funds.