Molecular Genomics Core

分子基因组学共享资源 USC Norris Comprehensive Cancer的分子基因组学（MGC）共享资源（SR）的使命 中心（NCCC）提供行业标准和最先进的分子和基因组分析服务 支持NCCC研究人员在基础、转化、临床和人口领域的连续工作 以理工科为重由NCCC管理局监督，并在John Carpten博士和大卫克雷格的领导下 博士，都是新招募到NCCC在当前周期，MGC作为一个基本要素与行业- 在大规模SNP基因分型，DNA甲基化阵列， 整合基因组测序在体细胞，转录组，和表观遗传景观，以及国家的- ART单细胞和空间分子谱分析。这一周期的主要发展包括获得更大的生产- 规模排序，确保抢先体验变革性新兴技术， 生物信息学过程整合到其他SR中。具体来说，MGC：1）增加了高通量测序 使用先进的机器人技术和Illumina NovaSeq从头开始构建手臂，以实现大规模项目; 2） 与行业合作伙伴建立了新的合作关系，以确保可用性和抢先体验高级状态- 最先进的单细胞和空间分子分析，包括10 X Genomics、Agilent和Nanostring;以及3） 实施自动化API、生物信息学和数据处理，以便与数据科学紧密协调 大规模Novaseq测序中的共享资源（DSC）。根据NCCC战略计划， 下一个资助周期，MGC计划进行全面的分子分析，并与策划的患者数据联系起来， 包括单细胞基因组学、用于筛查和残留疾病的液体活组织检查以及成像质量细胞术 和空间转录组学，以捕捉肿瘤和肿瘤微环境的相互作用，应用于NCCC的各种 患者人群。在目前的资助期（2015-2020年），122名NCCC成员代表所有五个 项目利用了MGC，产生了233篇出版物和5840万美元的直接成本补助资金 的使用。在下一个赠款周期的第一年，小额赠款委员会的预计年度预算为2，875，229美元，但 CCSG要求是457，972美元。因此，MGC利用广泛的机构和充值支持， 只从CCSG基金中寻求16%的资金。

PROJECT SUMMARY – Molecular Genomics Shared Resource The mission of the Molecular Genomics (MGC) Shared Resource (SR) of the USC Norris Comprehensive Cancer Center (NCCC) is to provide services for industry-standard and state-of-the-art molecular and genomic assays in support of NCCC investigators working across the continuum of basic, translational, clinical, and population sciences. Overseen by NCCC Administration and under the leadership of John Carpten PhD and David Craig PhD, both newly recruited to NCCC in the current cycle, the MGC serves as a foundational element with industry- standard technologies and field-leading expertise in large-scale SNP genotyping, DNA methylation arrays, integrated genomic sequencing at the somatic, transcriptomic, and epigenetic landscape as well as state-of-the- art single-cell and spatial molecular profiling. Major developments this cycle include access to larger production- scale sequencing, ensuring early access to transformative emerging technologies, and implementation of bioinformatic processes integration into other SRs. Specifically, MGC: 1) added a high-throughput sequencing arm built from the ground up with advanced robotics and Illumina NovaSeq to enable large-scale projects; 2) established new collaborations with industry partners to ensure availability and early access to advanced state- of-the-art single-cell and spatial molecular profiling, including 10X Genomics, Agilent, and Nanostring; and 3) implemented automated APIs, bioinformatics, and data processing for tight coordination with the Data Science Shared Resource (DSC) in large-scale Novaseq sequencing. In alignment with the NCCC Strategic Plan, in the next grant cycle, MGC plans to perform comprehensive molecular profiling with linkage to curated patient data, including single-cell genomics, liquid biopsies for screening and residual disease, and imaging mass cytometry and spatial transcriptomics to capture tumor and tumor microenvironment interactions applied to NCCC's diverse patient population. During the current grant period (2015-2020), 122 NCCC members representing all five programs utilized the MGC, resulting in 233 publications and $58.4M in direct cost grant funding as a direct result of usage. The anticipated annual budget of the MGC in the first year of the next grant cycle is $2,875,229, yet the CCSG request is $457,972. Accordingly, the MGC leverages extensive institutional and recharge support and seeks only 16% from CCSG funds.