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NHLBI MAINTENANCE OF CANINE MODELS OF HUMAN BLEEDING DISORDERS

NHLBI 人类出血性疾病犬模型的维护

基本信息

批准号：
10918015
负责人：
TIMOTHY NICHOLS
金额：
$ 142.25万
依托单位：
UNIV OF NORTH CAROLINA CHAPEL HILL
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-09-20 至 2024-09-19
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10918015
关键词：
Acceleration Animal Experimentation Animal Model Animals Antibodies Basic Science Biological Assay Biology Blood Blood Banks Blood Coagulation Disorders Blood Platelets Blood coagulation Blood donor Breeding Canada Canis familiaris Caring Clinical Clinical Trials Coagulation Process Collaborations Communities Contracts Defect Deletion Mutation Disease Dose Ensure Enzyme Precursors Erythrocytes Exons Experimental Designs F8 gene Freezing Funding Gene Transfer Gene therapy trial Genes Genetic Grant Hemophilia A Hemophilia B Hemorrhage Hemostatic Agents Hemostatic function Human Human Resources Inherited Introns Investigation Laboratory Research Maintenance Mentors Molecular National Heart, Lung, and Blood Institute Patients Peer Review Performance Persons Pharmaceutical Preparations Phenotype Plasma Production Proteins Publications R24 Recombinants Reporting Research Research Personnel Research Project Grants Resources Safety Science Services Site Support Contracts Technical Expertise Testing Therapeutic Thrombasthenia Thromboplastin Training Translational Research United States National Institutes of Health Whole Blood Work animal care blood product canine model clinical practice cost cost effective design editorial experience gain of function human model inhibitor inter-institutional novel novel therapeutics null mutation research study response symposium vector von Willebrand Disease von Willebrand Factor

项目摘要

The purpose of this N01 contract is to produce and maintain the following dog models of inherited bleeding disorders for independent and collaborative scientific investigations: hemophilia A and hemophilia B with and without inhibitory antibodies to FVIII and FIX, respectively; von Willebrand disease (VWD); FVII-deficiency; and Glanzmann’s Thrombasthenia. Beginning in 1947 with an R01 from NIH/NHLBI, Dr. Kenneth M. Brinkhous and colleagues identified these dogs and demonstrated that (1) they mirror the severe bleeder phenotype present in the respective human disorder and (2) have a strong predictive accuracy for successful translational research.1 In 1999, the reviewers of Dr. Brinkhous’ R01 judged these dogs to be an important national resource that should be made available to the research community and NHLBI support was transitioned to an R24 (T.C. Nichols, PI). We are now applying to transition support of this colony to this N01 contract. Since 1999, we have produced bleeder and normal dogs that have been used in collaborations with over 30 teams of investigators, most with NHLBI support, and the work has been reported in over 95 peer-reviewed publications1-96 and Conference Proceedings97-102, several of which were selected for editorial review. Recently, vectors used in at least 5 human gene therapy trials for hemophilia B and 2 for hemophilia A, six new recombinant anti-hemophilic proteins, and recombinant von Willebrand factor (VWF)and IL-1120 for VWD have been tested in these dogs prior to clinical trials. In all cases, the results predicted safety, efficacy, and drug dose responses. The primary benefits of these animals have been to accelerate translatable discovery science in coagulation and hemostasis research and to produce the scientific basis for the safe and efficacious introduction of novel therapeutics into clinical practice. Considerable progress has been made over the past 7 decades in understanding the basic science of blood coagulation and hemostasis and in developing new clinical approaches to inherited bleeding disorders. Yet, there are persistent limitations in therapeutic options for people with VWD and hemophilia, especially those with inhibitors.

这份N01合同的目的是为独立和合作的科学研究制作和维护以下遗传性出血性疾病的犬模型：分别带有和不带有FVIII和FIX抑制性抗体的血友病A和血友病B；von Willebrand病(VWD)；FVII缺乏；以及Glanzmann的血栓形成症。从1947年NIH/NHLBI的R01狗开始，Kenneth M.Brinkhous博士和他的同事鉴定了这些狗，并证明(1)它们反映了各自人类疾病中存在的严重出血表型，(2)对成功的翻译研究具有很强的预测准确性。1在1999年，Brinkhous博士的R01的评审者认为这些狗是应该向研究界提供的重要的国家资源，NHLBI的支持被过渡到R24(T.C.Nichols，Pi)。我们现在正在申请将这个殖民地的支持过渡到这份N01合同。自1999年以来，我们已经培育了出血犬和正常犬，用于与30多个研究团队的合作，其中大多数得到了NHLBI的支持，这项工作已在95个同行评议的出版物1-96和会议过程97-102上报道，其中几个被选为编辑审查。最近，至少5项针对血友病B和2项针对血友病A的人类基因治疗试验中使用的载体，6种新的重组抗血友病蛋白，以及针对VWD的重组von Willebrand因子(VWF)和IL-1120在临床试验之前已经在这些狗身上进行了测试。在所有病例中，结果都预测了安全性、有效性和药物剂量反应。这些动物的主要好处是加速了凝血和止血研究中的可翻译发现科学，并为安全有效地将新疗法引入临床实践提供了科学基础。在过去的70年里，在了解血液凝固和止血的基础科学以及开发遗传性出血疾病的新的临床方法方面，已经取得了相当大的进步。然而，VWD和血友病患者的治疗选择一直存在局限性，特别是那些使用抑制剂的人。