TGF-beta Regulation of Mucosal and Systemic T Cell Immunity and Tolerance

TGF-β 对粘膜和全身 T 细胞免疫和耐受的调节

• 批准号: 10920182
• 负责人: Wanjun Chen
• 金额: $ 215.03万
• 依托单位: NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10920182
• 关键词: Animal Model; Antigens; Apoptosis; Apoptotic; Attention; Autoimmune Diseases; Autoimmunity; Basic Science; Cells; Communicable Diseases; Dendritic Cells; Development; Disease; FOXP3 gene; Generations; Genes; Genetically Engineered Mouse; Glycolysis; Goals; IL2RA gene; Immune Tolerance; Immunity; Immunosuppression; Inflammation; Insulin-Dependent Diabetes Mellitus; Knockout Mice; Knowledge; Lymphocyte; Lymphoid; Lymphoid Tissue; Macrophage; Malignant Neoplasms; Mannose; Mediating; Metabolism; Mission; Molecular; Mucous Membrane; National Institute of Dental and Craniofacial Research; Oral cavity; Oral mucous membrane structure; Pathogenesis; Pathway interactions; Peptides; Peripheral; Play; Production; Pulmonary Inflammation; Regulation; Regulatory T-Lymphocyte; Research; Role; Sepsis; Signal Transduction; System; T cell regulation; T-Lymphocyte; Therapeutic Intervention; Thymus Gland; Time; Transforming Growth Factor beta; Transforming Growth Factor beta Receptors; autoimmune inflammation; design; human disease; interest; intraepithelial; malignant mouth neoplasm; mouse model; transcription factor; tumor; wound healing

Our research interest is to elucidate mechanisms of TGF-beta regulation of mucosal and systemic T cell immunity and tolerance, in order to offer targets to manipulate T cell immunity versus tolerance in animal models to develop potential therapies for relevant human diseases such as autoimmunity, cancer and infectious diseases. Specifically we would like to understand (1) how TGF-beta is involved in the function and development of CD4+CD25+ T regulatory cells (Treg) in mucosal and other lymphoid tissues, (2) how TGF-beta regulates T programmed cell death and the consequent immune tolerance, (3) how TGF-beta signal transduction and TGF-beta production in mucosal T cells are regulated, and (4) The cellular and molecular mechanisms of TGF-beta regulation of oral mucosal intraepithelial lymphocytes (IELs). Recently, we have focused on the isolation and characterization of CD4+CD25+ T regulatory cells from normal and genetically engineered mice and dissected the involvement of TGF-beta in immunosuppression mediated by T regulatory cells. Importantly, we have also identified that TGF-beta is a critical factor in the conversion of naive CD4+CD25- peripheral T cells to CD4+CD25+ T regulatory cells through induction of Foxp3, a master gene for T regulatory cell development. This finding not only has significant impact on understanding the generation of CD4+CD25+ regulatory T cells, but also makes it possible for the first time to design strategies to embellish the limited and/or inadequate numbers of T regulatory cells in the periphery, as needed, for therapeutic intervention in autoimmune diseases and inflammation. In addition, with the T-cell specific conditional TGF-beta receptor I knockout mice, we have shown that TGF-beta signaling is also critical for the development and generation of natural Foxp3+ Tregs in the thymus. In related studies, we have demonstrated that combination of clearance of apoptotic T cells by macrophages and immature dendritic cells with administration of autoantigenic peptide leads to generation of antigen-specific Tregs that in turn suppress autoimmunity and induce long-term immune tolerance. Furthermore, we have also discovered that D-mannose induces Treg cells and suppresses type I diabtes and lung inflammation in mouse models. Moreover, we have discovered two transcription factors DBP and E2F8 play an opposite functions in the differentiation of anti-tumor Th9 cells. We have also revealed that TGF-beta uncouples glycolysis and inflammation in macrophages and controls the survival during sepsis. Our immediate next steps are to decipher the molecular pathway(s) by which TGF-beta induces Foxp3 expression to define how CD4+CD25+ T cells are developed in and out of mucosal lymphoid systems, as well as to begin to resolve the mystery of TGF-beta's role in T regulatory cell mediated immune tolerance. We also intend to apply the knowledge obtained from our basic research in animal models to develop potential therapy for relevant human diseases including inflammation/autoimmune diseases, cancer and infectious diseases, with special attention to NIDCR mission relevant diseases such as wound healing, oral cavity associated inflammation and oral cancer.

我们的研究兴趣是阐明TGF-β调节粘膜和系统性T细胞免疫和耐受的机制，以提供在动物模型中操纵T细胞免疫与耐受的靶点，从而开发用于相关人类疾病如自身免疫、癌症和感染性疾病的潜在疗法。具体而言，我们希望了解（1）TGF-β如何参与粘膜和其他淋巴组织中CD 4 + CD 25 + T调节细胞（Treg）的功能和发育，（2）TGF-β如何调节T细胞程序性死亡和随后的免疫耐受，（3）如何调节粘膜T细胞中的TGF-β信号转导和TGF-β产生，口腔黏膜上皮内淋巴细胞（IELs）TGF-β调节的细胞和分子机制。 最近，我们集中于从正常和基因工程小鼠中分离和鉴定CD 4 + CD 25 + T调节细胞，并剖析了TGF-β参与T调节细胞介导的免疫抑制。重要的是，我们还确定了TGF-β是通过诱导Foxp 3（T调节细胞发育的主基因）将初始CD 4 + CD 25-外周T细胞转化为CD 4 + CD 25 + T调节细胞的关键因子。这一发现不仅对理解CD 4 + CD 25+调节性T细胞的产生具有重大影响，而且还首次使设计策略以根据需要修饰外周中有限和/或不足数量的T调节性细胞成为可能，用于自身免疫性疾病和炎症的治疗干预。 此外，通过T细胞特异性条件性TGF-β受体I敲除小鼠，我们已经表明TGF-β信号传导对于胸腺中天然Foxp 3 + TcB的发育和产生也是至关重要的。在相关研究中，我们已经证明，巨噬细胞和未成熟树突状细胞清除凋亡T细胞与给予自身抗原肽的组合导致抗原特异性T细胞的产生，从而抑制自身免疫并诱导长期免疫耐受。此外，我们还发现D-甘露糖在小鼠模型中诱导Treg细胞并抑制I型糖尿病和肺部炎症。 此外，我们还发现两种转录因子DBP和E2 F8在抗肿瘤Th 9细胞的分化中起相反的作用。我们还发现，TGF-β解偶联糖酵解和巨噬细胞中的炎症，并控制脓毒症期间的存活。我们的下一步是破译TGF-β诱导Foxp 3表达的分子途径，以确定CD 4 + CD 25 + T细胞如何在粘膜淋巴系统内外发育，以及开始解决TGF-β在T调节细胞介导的免疫耐受中的作用之谜。我们还打算将从动物模型基础研究中获得的知识应用于开发相关人类疾病的潜在治疗方法，包括炎症/自身免疫性疾病，癌症和感染性疾病，特别关注NIDCR使命相关疾病，如伤口愈合，口腔相关炎症和口腔癌。

项目成果

Corrigendum to "Oral cancer-derived exosomal NAP1 enhances cytotoxicity of natural killer cells via the IRF-3 pathway" [Oral Oncol. 76 (2018) 34-41].

“口腔癌衍生的外泌体 NAP1 通过 IRF-3 途径增强自然杀伤细胞的细胞毒性”的勘误 [Oral Oncol.

• DOI: 10.1016/j.oraloncology.2018.01.007
• 发表时间: 2018
• 期刊: Oral oncology
• 影响因子: 4.8
• 作者: Wang,Yingnan;Qin,Xing;Zhu,Xueqin;Chen,Wanjun;Zhang,Jianjun;Chen,Wantao
• 通讯作者: Chen,Wantao

The DNA-binding inhibitor Id3 regulates IL-9 production in CD4(+) T cells.

• DOI: 10.1038/ni.3252
• 发表时间: 2015-10
• 期刊: Nature immunology
• 影响因子: 30.5
• 作者: Nakatsukasa H;Zhang D;Maruyama T;Chen H;Cui K;Ishikawa M;Deng L;Zanvit P;Tu E;Jin W;Abbatiello B;Goldberg N;Chen Q;Sun L;Zhao K;Chen W
• 通讯作者: Chen W

Prestimulation of CD2 confers resistance to HIV-1 latent infection in blood resting CD4 T cells.

• DOI: 10.1016/j.isci.2021.103305
• 发表时间: 2021-11-19
• 期刊: iScience
• 影响因子: 5.8
• 作者: He S;Guo J;Fu Y;Spear M;Qin C;Fu S;Cui Z;Jin W;Xu X;Chen W;Shang H;Wu Y
• 通讯作者: Wu Y

The molecular mechanisms of Foxp3 gene regulation.

• DOI: 10.1016/j.smim.2011.06.005
• 发表时间: 2011-12
• 期刊: Seminars in immunology
• 影响因子: 7.8
• 作者: Maruyama T;Konkel JE;Zamarron BF;Chen W
• 通讯作者: Chen W

TGF-beta1 on osteoimmunology and the bone component cells.

• DOI: 10.1186/2045-3701-3-4
• 发表时间: 2013-01-15
• 期刊: Cell & bioscience
• 影响因子: 7.5
• 作者: Kasagi S;Chen W
• 通讯作者: Chen W