Core 2: Mammalian Artificial Chromosome (MAC)

核心 2:哺乳动物人工染色体 (MAC)

基本信息

  • 批准号:
    10626286
  • 负责人:
  • 金额:
    $ 36.48万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2023
  • 资助国家:
    美国
  • 起止时间:
    2023-05-08 至 2028-04-30
  • 项目状态:
    未结题

项目摘要

The mammalian artificial chromosome (MAC) Core will support the overall Project, which seeks to address the downstream consequences in cells and animals of DNA damage, the causes and consequences of chromosome missegregation at cell division, and the mechanisms that drive these processes in tumors. It will serve as a foundry for MACs that will support each of the individual projects that require chromosome manipulations and engineering: from site-directed and inducible DNA damage, to reporters for chromosome segregation errors, to housing the genetically encoded components of a chemical genetics toolbox. MACs can carry large (i.e. 100-2000 kb) genetic payloads, and exist in cells as molecules that are propagated and inherited alongside the natural chromosomes. Their autonomous nature is a key advantage, since they deliver their genetic payloads without insertion or other disruption of the host genome. The Core will employ our recent innovations in MAC technology that gave rise to a new generation of MAC templates in which we direct the seeding of centromeric chromatin. This approach permits efficient MAC formation on diverse DNA templates. The Core will undertake MAC approaches from their design, creation, delivery into cells, characterization, through to their implementation for experiments proposed in the individual Projects of the program. Specifically, the Core will develop and use MACs that fall into the following categories: ones that serve as targets for DNA damage, ones that harbor elaborate genetic payloads that will modulate chromosome segregation with a high degree of sophistication, and ones that carry fluorescent reporters of chromosome missegregation. The MAC Core is designed to be flexible to help Project members develop new lines of experimentation that arise from the findings achieved during the initial steps in the overall program.
哺乳动物人工染色体(MAC)核心将支持整个项目,该项目旨在解决

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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Ben E. Black其他文献

A high-resolution look at fresh human brain vitrified directly from autopsy using cryo-electron tomography with cryo-plasma focused ion beam milling
  • DOI:
    10.1016/j.bpj.2023.11.1192
  • 发表时间:
    2024-02-08
  • 期刊:
  • 影响因子:
  • 作者:
    Benjamin C. Creekmore;Kathryn Kixmoeller;Ben E. Black;Edward B. Lee;Yi-Wei Chang
  • 通讯作者:
    Yi-Wei Chang
A PARP2 active site helix melts to permit DNA damage-induced enzymatic activation
PARP2 活性位点螺旋熔化以允许 DNA 损伤诱导的酶促激活
  • DOI:
    10.1016/j.molcel.2025.01.004
  • 发表时间:
    2025-03-06
  • 期刊:
  • 影响因子:
    16.600
  • 作者:
    Emily S. Smith-Pillet;Ramya Billur;Marie-France Langelier;Tanaji T. Talele;John M. Pascal;Ben E. Black
  • 通讯作者:
    Ben E. Black
Remodeling of inner kinetochore components at mitotic onset is required for chromosome segregation
  • DOI:
    10.1016/j.bpj.2023.11.2240
  • 发表时间:
    2024-02-08
  • 期刊:
  • 影响因子:
  • 作者:
    Praveen Kumar Allu;Lucie Y. Guo;Rachel M. Lackner;David M. Chenoweth;Ben E. Black
  • 通讯作者:
    Ben E. Black
Satellite DNA shapes dictate pericentromere packaging in female meiosis
卫星 DNA 形状决定着女性减数分裂中着丝粒周围区域的包装
  • DOI:
    10.1038/s41586-024-08374-0
  • 发表时间:
    2025-01-08
  • 期刊:
  • 影响因子:
    48.500
  • 作者:
    Damian Dudka;Jennine M. Dawicki-McKenna;Xueqi Sun;Keagan Beeravolu;Takashi Akera;Michael A. Lampson;Ben E. Black
  • 通讯作者:
    Ben E. Black
Centromeric chromatin clearings demarcate the site of kinetochore formation
着丝粒染色质间隙划定了动粒形成的位点。
  • DOI:
    10.1016/j.cell.2024.12.025
  • 发表时间:
    2025-03-06
  • 期刊:
  • 影响因子:
    42.500
  • 作者:
    Kathryn Kixmoeller;Ekaterina V. Tarasovetc;Elie Mer;Yi-Wei Chang;Ben E. Black
  • 通讯作者:
    Ben E. Black

Ben E. Black的其他文献

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{{ truncateString('Ben E. Black', 18)}}的其他基金

Tuning PARP-1 retention and release on DNA breaks
调节 DNA 断裂时 PARP-1 的保留和释放
  • 批准号:
    10363534
  • 财政年份:
    2022
  • 资助金额:
    $ 36.48万
  • 项目类别:
Tuning PARP-1 retention and release on DNA breaks
调节 DNA 断裂时 PARP-1 的保留和释放
  • 批准号:
    10581522
  • 财政年份:
    2022
  • 资助金额:
    $ 36.48万
  • 项目类别:
Mendelian inheritance of artificial chromosomes
人工染色体的孟德尔遗传
  • 批准号:
    10666591
  • 财政年份:
    2021
  • 资助金额:
    $ 36.48万
  • 项目类别:
Mendelian inheritance of artificial chromosomes
人工染色体的孟德尔遗传
  • 批准号:
    10487450
  • 财政年份:
    2021
  • 资助金额:
    $ 36.48万
  • 项目类别:
Mendelian inheritance of artificial chromosomes
人工染色体的孟德尔遗传
  • 批准号:
    10272686
  • 财政年份:
    2021
  • 资助金额:
    $ 36.48万
  • 项目类别:
Centromere identity, strength, and regulation
着丝粒的身份、强度和调节
  • 批准号:
    10368979
  • 财政年份:
    2019
  • 资助金额:
    $ 36.48万
  • 项目类别:
Centromere identity, strength, and regulation
着丝粒的身份、强度和调节
  • 批准号:
    10175347
  • 财政年份:
    2019
  • 资助金额:
    $ 36.48万
  • 项目类别:
Structural biology and molecular biophysics training program
结构生物学和分子生物物理学培训计划
  • 批准号:
    10630348
  • 财政年份:
    2019
  • 资助金额:
    $ 36.48万
  • 项目类别:
Centromere identity, strength, and regulation
着丝粒的身份、强度和调节
  • 批准号:
    9896871
  • 财政年份:
    2019
  • 资助金额:
    $ 36.48万
  • 项目类别:
Structural biology and molecular biophysics training program
结构生物学和分子生物物理学培训计划
  • 批准号:
    10192759
  • 财政年份:
    2019
  • 资助金额:
    $ 36.48万
  • 项目类别:

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