West Africa - Tulane University Laboratory Training Fellowship Supplement for Partnership for Research in Emerging Viral Infections Sierra Leone (PREVSL) U2RTW011248

项目摘要/摘要：我们在这里描述我们的补充资金的建议，以制定一个3个月， 实验室为基础，指导实习奖学金，总部设在杜兰大学在新奥尔良，路易斯安那州， 提供西非博士候选人在细胞培养，假病毒， 生产、免疫细胞分析、细胞内-细胞外染色、Luminex免疫测定、基因组学和 转录组学分析PREVSL PI Moon和Schieffelin参与了区域协调会议 在技术工作组中。RETAIN是一个广泛的利益相关者联盟，代表美国。 政府和国际学术机构。每月举行两次虚拟会议， 年，目标是协助利比里亚大学健康科学学院（ULCHS）增加其 研究生产力和更广泛地改善利比里亚的医疗和公共卫生教育。此夕h RETAIN工作组尽可能利用美国政府和其他卫生投资， 在西非，卫生科学研究中心正在与该地区的卫生科学研究中心合作，以便在西非更广泛地建设卫生科学研究能力。西非-杜兰 大学实验室培训奖学金建立在ULCHS和RETAIN之间最近的战略规划基础上， 提供所需的先进的实验室培训，以完成细胞和分子生物学博士学位。 该实验室培训奖学金将补充通过我们开发的研究能力建设活动。 塞拉利昂新发病毒感染研究伙伴关系（PREVSL） U2RTW011248。在这方面，在加纳大学完成博士学位的利比里亚研究员Kalilu Donzo 已被选为研究员根据这一建议，如果它的资金。这个导师密集奖学金 包括在新奥尔良杜兰大学的Schieffelin实验室工作三个月， 路易斯安那州，美国，以获得本文所述的实验室技能，并需要完成博士论文 研究标题：麻疹病毒在接种疫苗的个体中的免疫学和遗传学特征 在利比里亚重新爆发疫情期间。

Project Summary/Abstract: We describe here our proposal for supplemental funding to develop a 3-month, laboratory-based, mentored practicum fellowship, based at Tulane University in New Orleans, Louisiana to provide a West African doctoral candidate with hands-on practical experience in cell culture, pseudovirus production, immune cell profiling, intra-extra cellular staining, Luminex immune-assay, genomics, and transcriptomic profiling. PREVSL PIs Moon and Schieffelin have been involved in regional coordination meetings within the RETAIN technical working group. RETAIN is a broad coalition of stakeholders representing the U.S. Government and international academic institutions. Virtual meetings have been held twice monthly for several years, with a goal of assisting the University of Liberia College of Health Sciences (ULCHS) in increasing its research productivity and improving medical and public health education in Liberia more broadly. Further, the RETAIN working group, whenever possible, leverages U.S. Government and other health investments in the region in order to build health science research capacity more broadly in West Africa. The West Africa -Tulane University Laboratory Training Fellowship builds on recent strategic planning between ULCHS and RETAIN by providing needed advanced laboratory training towards the completion of a PhD in Cell and Molecular Biology. This Laboratory Training Fellowship will compliment research capacity building activities developed through out parent grant, titled Partnership for Research in Emerging Viral Infections Sierra Leone (PREVSL) U2RTW011248. In this regard, Kalilu Donzo, a Liberian researcher completing his PhD at the University of Ghana has been selected as a Fellow under this proposal should it be funded. This mentorship intensive Fellowship includes three-months embedded within the Schieffelin laboratory at Tulane University in New Orleans, Louisiana, USA to gain the laboratory skills described herein, and that are needed to complete his doctoral thesis research titled: Immunologic and genetic characterization of Measles Virus in vaccinated individuals during a re-emergence outbreak in Liberia.