West Africa - Tulane University Laboratory Training Fellowship Supplement for Partnership for Research in Emerging Viral Infections Sierra Leone (PREVSL) U2RTW011248

项目摘要/摘要：我们在这里介绍我们的补充资金建议，以开发一个为期3个月的 在路易斯安那州新奥尔良的杜兰大学，以实验室为基础，有指导的实践奖学金 为西非博士生提供细胞培养、假病毒方面的实践经验 生产、免疫细胞图谱、细胞内外染色、Luminex免疫分析、基因组学和 转录剪裁侧写。PREVSL PIs Moon和Schieffelin参加了区域协调会议 在保留技术工作组内。Retain是代表美国的利益相关者的广泛联盟。 政府和国际学术机构。虚拟会议每月举行两次，已有几次 几年来，目标是协助利比里亚大学健康科学学院(ULCHS)增加其 更广泛地研究生产力和改善利比里亚的医疗和公共卫生教育。此外， 保留工作组，只要可能，就利用美国政府和其他卫生投资 教科文组织将继续在该区域开展活动，以便在西非更广泛地建设卫生科学研究能力。西非-杜兰 大学实验室培训奖学金建立在ULCHS和RESTER BY之间最近的战略规划基础上 提供必要的高级实验室培训，以完成细胞和分子生物学博士学位。 这项实验室培训奖学金将补充通过OUT开展的研究能力建设活动 父母基金，题为塞拉利昂新出现病毒感染研究伙伴关系(PREVSL) U2RTW011248。在这方面，在加纳大学完成博士学位的利比里亚研究人员Kalilu Donzo 已经被选为这项提案的研究员，如果它得到资助的话。这种导师式的密集团契 包括在新奥尔良杜兰大学希夫林实验室内植入三个月， 美国路易斯安那州，获得本文所述的实验室技能，完成他的博士论文所需的技能 研究题为：麻疹病毒在接种疫苗后的免疫学和基因特征 在利比里亚再次爆发疫情期间。

Project Summary/Abstract: We describe here our proposal for supplemental funding to develop a 3-month, laboratory-based, mentored practicum fellowship, based at Tulane University in New Orleans, Louisiana to provide a West African doctoral candidate with hands-on practical experience in cell culture, pseudovirus production, immune cell profiling, intra-extra cellular staining, Luminex immune-assay, genomics, and transcriptomic profiling. PREVSL PIs Moon and Schieffelin have been involved in regional coordination meetings within the RETAIN technical working group. RETAIN is a broad coalition of stakeholders representing the U.S. Government and international academic institutions. Virtual meetings have been held twice monthly for several years, with a goal of assisting the University of Liberia College of Health Sciences (ULCHS) in increasing its research productivity and improving medical and public health education in Liberia more broadly. Further, the RETAIN working group, whenever possible, leverages U.S. Government and other health investments in the region in order to build health science research capacity more broadly in West Africa. The West Africa -Tulane University Laboratory Training Fellowship builds on recent strategic planning between ULCHS and RETAIN by providing needed advanced laboratory training towards the completion of a PhD in Cell and Molecular Biology. This Laboratory Training Fellowship will compliment research capacity building activities developed through out parent grant, titled Partnership for Research in Emerging Viral Infections Sierra Leone (PREVSL) U2RTW011248. In this regard, Kalilu Donzo, a Liberian researcher completing his PhD at the University of Ghana has been selected as a Fellow under this proposal should it be funded. This mentorship intensive Fellowship includes three-months embedded within the Schieffelin laboratory at Tulane University in New Orleans, Louisiana, USA to gain the laboratory skills described herein, and that are needed to complete his doctoral thesis research titled: Immunologic and genetic characterization of Measles Virus in vaccinated individuals during a re-emergence outbreak in Liberia.