DEVELOPMENT OF INTRINSIC CONNECTIONS OF THE BARREL CORTEX

桶状皮层内在连接的发展

• 批准号: 6112158
• 负责人: THOMAS A WOOLSEY
• 金额: $ 10.3万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-06-01 至 2000-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6112158
• 关键词: action potentials; brain mapping; cerebral cortex; developmental neurobiology; fluorescent dye /probe; histology; intercellular connection; laboratory mouse; sensory deprivation; sensory mechanism; somesthetic sensory cortex

The rodent trigeminal system has favorable characteristics for elucidating mechanisms of development of brain pathways and order in them. Each whisker on the face projects to a discrete cell group in the brain. In the cerebral cortex these are called barrels. The ensemble of barrels makes a map of the animal that can be recognized in tissue sections with or without stains. We propose to exploit the map that the barrels provide, the postnatal development of the intracortical connections within the map and the sensitivity of that development to changes in sensory input to clarify how specific intracortical circuits required for integration of inputs from the whiskers develop. We will examine the following hypotheses: . Hypothesis I: there is a sequence of development of intracortical (interbarrel) projections that is lamina specific, . Hypothesis II: connection to the ordered periphery is necessary for the proper patterns to develop, . Hypothesis III: the effect of selected whisker removal is asymmetrical at the boundaries between connected barrels and disconnected barrels which are at a competitive disadvantage, . Hypothesis IV: normal neuronal activity plays a role in the development of interbarrel connections, . Hypothesis V: intracortical action potentials play the major role in the development of the interbarrel connections. We will use lipophilic dyes, targeted under direct vision to individual barrels or to particular cortical layers over and under individual layer IV barrels and other transported markers, to demonstrate intracortical connectivity. The project will use CORE C to digitize and visualize results of fluorescent dye transport, CORE B to quantitate these results and the results from transported marker studies and CORE E to align findings from serial sections. These studies relate directly to work in Projects 4, 6, and 7. The goal is to understand the manner in which intracortical communication. The work can contribute to clarifying mechanisms that may be important for understanding mental retardation, epilepsy and learning disabilities.

啮齿动物三叉神经系统具有有利的特征， 阐明大脑通路和顺序的发展机制， 他们 表面上的每个须状物都投射到细胞中的离散细胞群中。 个脑袋 在大脑皮层中，这些被称为桶。 合奏 桶的形状构成了动物的地图， 有或没有污渍的部分。 我们建议利用地图， 桶提供，出生后的发展， 地图内的联系以及这种发展对 感觉输入的变化，以阐明特定的皮质内回路 所需的集成输入从晶须的发展。 我们将 检查以下假设： .假设一：皮质内的发育有一个顺序， （管间）突出物是椎板特异性的， .假设二：与有序外围的联系是必要的， 形成正确的模式， .假设三：选择性晶须去除效果不对称 在连接的桶和断开的桶之间的边界处 处于竞争劣势， .假设四：正常的神经元活动在神经系统中起着重要作用。 发展枪管间的连接， .假设五：皮质内动作电位在脑内活动中起主要作用。 枪管间连接的发展。 我们将使用亲脂性染料，在直接视觉下针对个人 桶或个别层之上和之下的特定皮质层 IV桶和其他运输标记，以证明皮质内 连通性。 该项目将使用CORE C来实现图形化和可视化 荧光染料转运结果，CORE B用于定量这些结果 以及来自转运标记研究和CORE E的结果， 系列切片的发现。 这些研究直接关系到 项目4、6和7。 我们的目标是了解 皮质内通讯 这项工作有助于澄清 可能对理解智力迟钝很重要的机制， 癫痫和学习障碍。