DEVELOPMENT AND PLASTICITY OF THALAMOCORTIC CELLS

丘脑皮质细胞的发育和可塑性

• 批准号: 6270268
• 负责人: THOMAS A WOOLSEY
• 金额: $ 13.77万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-02-15 至 1999-02-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6270268
• 关键词: afferent nerve; cell cell interaction; dendrites; denervation; developmental neurobiology; histology; laboratory rat; neural information processing; neural plasticity; neurogenesis; sensory deprivation; sensory feedback; somesthetic sensory cortex; thalamic nuclei; thalamocortical tract; trigeminal nerve; vibrissae

This project concerns two aspects of mouse specific thalamic relay cells (TCRs) from the whisker "barreloids" in the ventroposterior medial nucleus (VPM) to their targets the whisker "barrels" in the somatosensory cortex; specifically the denfrites and axon terminasl (thalamocortical afferents+TCAs) of these neurons. General Hypothesis I. TCR dendrites define different roles in development and maturity: Studies of TCR dendrite organization in VPM. We suggest that an early TCR role is to transmit pattern information from the brainstem to the cortex; a later rrole is to incorporate information from different whiskers. TCRs in fixed slices through VPM will be stained by intracellular injections of dye and reconstructed and measured with a computer microscope to: 1) determine directly the relationship of TCR dendrites to segregated afferents from brainstem trigeminal nuclei; 2) follow the postnatal development of TCR dendrites including documentating functional correlates of anatomical changes; and, 3) demonstrate the TCR dendrites are plastic and can be changed by altering the class or pattern of inputs to VPM from the brainstem. General hypothesis II. TCA grouping in development is a population phenomenon; Studies of TCA organization in barrel cortex. We suggest that the rich and dense afferent patterns from the barreloids to the barrels arises in part from interactions with each other. TCAs will be stained in bulk and signly by dyes in living and fixed materials and analyzed with quantitative confocal and ocmputer microscopy to; 1) determine the extent to which single fibers and groups of fibers to particular barrels share the same characteristics; 2) follow the development of the clustering of these fibers in early postnatal life; and, 30 demonstrate differences and similarities between fibers supplying the cortex of animals deafferented since bith and those where the pattern of TCA inputs is perturbed by selective whisker removal. The project will use the St. Louiis CORE for intracellular staining, three dimensional measurements of processes and for quantitation of TCAs. We will rely on the expertise of many of the other investigators in this project for advice, guidance and assistance with targeting markers, recording and collecting and analyzing the data. This project is related to higher-order processing of trigeminal information and its disturbances with environmental modifications to the nervous system.

本项目涉及小鼠特异性丘脑中继细胞的两个方面 腹后内侧核中的须状“桶样”（TCR） (VPM)它们的目标是躯体感觉皮层中的触须“桶”; 特别是树突和轴突终末（丘脑皮质 这些神经元的传入+TCAs）。 一般假设I. TCR树突在发育中定义了不同的角色 VPM中TCR枝晶组织的研究。 我们建议 早期TCR的作用是将模式信息从 脑干到皮层;后来的角色是将信息从 不同的胡须 通过VPM固定切片中的TCR将通过 细胞内注射染料，并用 计算机显微镜下观察：1）直接确定TCR的关系 树突到脑干三叉神经核的分离传入; 2） 跟踪TCR树突的出生后发育，包括记录 解剖学变化的功能相关性;以及，3）证明TCR 树突是可塑的，可以通过改变种类或模式来改变 从脑干到VPM的输入。 一般假设二。 发展中的TCA分组是一个群体， 桶状皮质中TCA组织的研究。 我们建议 从桶状体到桶的丰富而密集的传入模式 部分来自于彼此之间的相互作用。 TCA将在 活的和固定的材料中的大量和显着的染料，并分析与 定量共聚焦和计算机显微镜，以：1）确定 单个纤维和纤维组与特定的桶共享 （2）遵循这些集群的发展; 纤维在出生后早期的生活;和，30证明差异， 切断传入神经的动物大脑皮层的纤维之间的相似性 因为bith和那些TCA输入模式被干扰的情况下， 选择性晶须去除。 该项目将使用圣路易斯CORE进行细胞内染色， 工艺尺寸测量和TCA定量。 我们 将依赖于许多其他调查人员的专业知识， 关于目标标记的咨询、指导和援助项目， 记录、收集和分析数据。 该项目与 三叉神经信息的高阶处理及其干扰 环境对神经系统的影响