CONTROL OF FLOOR PLATE INDUCTION BY AXIAL MESODERM

轴向中胚层对底板感应的控制

• 批准号: 6243711
• 负责人: JANE DODD
• 金额: $ 0.52万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-05-01 至 1997-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6243711
• 关键词: bioassay; biological signal transduction; cell differentiation; chemical structure function; chick embryo; developmental neurobiology; embryo /fetus; gene expression; gene induction /repression; laboratory mouse; laboratory rabbit; laboratory rat; messenger RNA; molecular cloning; neural plate /tube; neurogenesis; polymerase chain reaction; protein structure function; quail; vertebrate embryology

The goal of the experiments to be described in this proposal is to elucidate the cellular and molecular events underlying the induction of the floor plate by the notochord. The floor plate is a specialized set of neuroepithelial cells located at the ventral midline of the embryonic spinal cord. The floor plate appears to be responsible for the differentiation and organization of neurons within the ventral spinal cord and, at later stages of development, plays a pivotal role in the guidance of axons within the spinal cord. We have previously shown that the differentiation of functional and antigenic properties that are specific to the floor plate is dependent on an inductive signal from the underlying notochord. The experiments described here use a simple assay system to measure the induction of floor plate-specific gene expression in cells of the lateral neural plate that would not normally give rise to floor plate. This assay will be used to examine the developmental expression pattern and biochemical properties of the notochord-derived signal and as a detection method in the identification of the activity. We will use an expression cloning approach to identify cDNAs that encode the floor plate-inducing activity. In the longer term, the goal is to understand the mechanisms by which neuroepithelial cells respond to the notochordal signal and initiate a program of floor plate differentiation. The identification of a notochord-derived floor plate-inducing signal and the elucidation of its mechanisms of action will be an important step in the understanding of early inductive processes and in the differentiation and organization of the early neural tube.

本提案中要描述的实验的目的是 阐明诱导的基础的细胞和分子事件 脊索板的地板板。 地板是一组专业的 位于胚胎的腹部中线的神经上皮细胞 脊髓。 地板板似乎是负责的 腹侧脊髓内神经元的分化和组织 并且，在发展的后期阶段，在指导中起着关键作用 脊髓内的轴突。 我们以前已经表明 特定于功能和抗原特性的分化 地板板取决于基础的电感信号 脊索。 此处描述的实验使用一个简单的测定系统 测量地板特异性基因表达在细胞中的诱导 通常不会产生地板板的外侧神经板。 该测定将用于检查发展表达模式和 脊索衍生信号的生化特性和检测 识别活动的方法。 我们将使用表达式 克隆方法识别编码地板诱导的cDNA 活动。 从长远来看，目标是通过 哪些神经上皮细胞对垂直信号响应并开始 地板差异的程序。 识别诺修（Notochord）的诱导信号和 阐明其行动机制将是重要的一步 对早期归纳过程和差异化的理解 以及早期神经管的组织。