CONTROL OF FLOOR PLATE INDUCTION BY AXIAL MESODERM
轴向中胚层对底板感应的控制
基本信息
- 批准号:6243711
- 负责人:
- 金额:$ 0.52万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1996
- 资助国家:美国
- 起止时间:1996-05-01 至 1997-12-31
- 项目状态:已结题
- 来源:
- 关键词:bioassay biological signal transduction cell differentiation chemical structure function chick embryo developmental neurobiology embryo /fetus gene expression gene induction /repression laboratory mouse laboratory rabbit laboratory rat messenger RNA molecular cloning neural plate /tube neurogenesis polymerase chain reaction protein structure function quail vertebrate embryology
项目摘要
The goal of the experiments to be described in this proposal is to
elucidate the cellular and molecular events underlying the induction of the
floor plate by the notochord. The floor plate is a specialized set of
neuroepithelial cells located at the ventral midline of the embryonic
spinal cord. The floor plate appears to be responsible for the
differentiation and organization of neurons within the ventral spinal cord
and, at later stages of development, plays a pivotal role in the guidance
of axons within the spinal cord. We have previously shown that the
differentiation of functional and antigenic properties that are specific to
the floor plate is dependent on an inductive signal from the underlying
notochord. The experiments described here use a simple assay system to
measure the induction of floor plate-specific gene expression in cells of
the lateral neural plate that would not normally give rise to floor plate.
This assay will be used to examine the developmental expression pattern and
biochemical properties of the notochord-derived signal and as a detection
method in the identification of the activity. We will use an expression
cloning approach to identify cDNAs that encode the floor plate-inducing
activity. In the longer term, the goal is to understand the mechanisms by
which neuroepithelial cells respond to the notochordal signal and initiate
a program of floor plate differentiation.
The identification of a notochord-derived floor plate-inducing signal and
the elucidation of its mechanisms of action will be an important step in
the understanding of early inductive processes and in the differentiation
and organization of the early neural tube.
本提案所述实验的目的是
阐明细胞和分子事件的诱导下,
脊索旁的地板 地板是一套专门的
位于胚胎腹侧中线的神经上皮细胞
脊髓 地板似乎是造成
腹侧脊髓内神经元的分化和组织
在发展的后期阶段,
脊髓内的轴突。 我们之前已经证明,
特异性的功能和抗原特性的差异,
底板取决于来自下层的感应信号
脊索 这里描述的实验使用简单的测定系统,
测量细胞中底板特异性基因表达的诱导,
外侧神经板通常不会形成底板。
该测定将用于检查发育表达模式,
脊索衍生信号的生物化学性质,并作为一种检测
方法在活动的识别。 我们将使用一个表达式
克隆方法鉴定编码地板诱导的cDNA
活动 从长远来看,我们的目标是通过以下方式了解这些机制:
哪些神经上皮细胞对脊索信号作出反应,
地板差异化方案。
脊索地板诱导信号的识别及
阐明其作用机制将是一个重要步骤,
对早期诱导过程和分化过程的理解
和早期神经管的组织。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
JANE DODD其他文献
JANE DODD的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('JANE DODD', 18)}}的其他基金
Linking the molecular logic of sensory neuron diversity and somatosensory circuitry in mouse spinal cord: development of novel tools for viral tracing and 3D analysis
将小鼠脊髓感觉神经元多样性的分子逻辑与体感回路联系起来:开发病毒追踪和 3D 分析的新工具
- 批准号:
10307604 - 财政年份:2020
- 资助金额:
$ 0.52万 - 项目类别:
Linking the molecular logic of sensory neuron diversity and somatosensory circuitry in mouse spinal cord: development of novel tools for viral tracing and 3D analysis
将小鼠脊髓感觉神经元多样性的分子逻辑与体感回路联系起来:开发病毒追踪和 3D 分析的新工具
- 批准号:
10156647 - 财政年份:2020
- 资助金额:
$ 0.52万 - 项目类别:
Trans-Synaptic Tracing of Developing Somatosensory Circuits
发育中的体感回路的跨突触追踪
- 批准号:
9066828 - 财政年份:2015
- 资助金额:
$ 0.52万 - 项目类别:
INDUCTION AND PATTERNING OF NEURAL TUBE VENTRAL MIDLINE
神经管腹侧中线的感应和图案化
- 批准号:
6565238 - 财政年份:2001
- 资助金额:
$ 0.52万 - 项目类别:
INDUCTION AND PATTERNING OF NEURAL TUBE VENTRAL MIDLINE
神经管腹侧中线的感应和图案化
- 批准号:
6410641 - 财政年份:2000
- 资助金额:
$ 0.52万 - 项目类别:
INDUCTION AND PATTERNING OF NEURAL TUBE VENTRAL MIDLINE
神经管腹侧中线的感应和图案化
- 批准号:
6302809 - 财政年份:1999
- 资助金额:
$ 0.52万 - 项目类别:
INDUCTION AND PATTERNING OF NEURAL TUBE VENTRAL MIDLINE
神经管腹侧中线的感应和图案化
- 批准号:
6112406 - 财政年份:1998
- 资助金额:
$ 0.52万 - 项目类别:
INDUCTION AND PATTERNING OF NEURAL TUBE VENTRAL MIDLINE
神经管腹侧中线的感应和图案化
- 批准号:
6273803 - 财政年份:1998
- 资助金额:
$ 0.52万 - 项目类别:
INDUCTION AND PATTERNING OF NEURAL TUBE VENTRAL MIDLINE
神经管腹侧中线的感应和图案化
- 批准号:
6296945 - 财政年份:1998
- 资助金额:
$ 0.52万 - 项目类别:
相似海外基金
ROLE OF CELL ADHESION IN BIOLOGICAL SIGNAL TRANSDUCTION
细胞粘附在生物信号转导中的作用
- 批准号:
6238317 - 财政年份:1997
- 资助金额:
$ 0.52万 - 项目类别:
ROLE OF CELL ADHESION IN BIOLOGICAL SIGNAL TRANSDUCTION
细胞粘附在生物信号转导中的作用
- 批准号:
5210031 - 财政年份:
- 资助金额:
$ 0.52万 - 项目类别: