MINORITY SCIENTIST AWARD AT MINORITY INSTITUTIONS

该项目的长期目标是培养R博士。蕾妮·里姆斯 一个独立的生物医学研究者， 在选定的研究导师的实验室学习， 这些数据可用于申请R 01研究基金。研究 将在Raymond F博士处提供指导。诺瓦克的实验室在 韦恩州立大学化学毒理学研究所。技术 Reams博士学到的知识将帮助她定义和研究问题， 阐明“铅”对哺乳动物系统的作用机制。他 Reams博士将在Novak博士的实验室学习的具体技术 包括原代肝细胞细胞培养以及传代， 细胞系，蛋白质印迹分析，总RNA和多聚（A）+ RNA分离， 北方印迹分析，核运行分析，逆转录酶 聚合酶链反应、限制性酶切分析、克隆和DNA测序 分析.这些当代细胞和分子生物学的方法将 在佛罗里达A&M大学的实验室里， 研究“铅对酪氨酸羟化酶基因表达的影响， 肾上腺和神经细胞中的信号转导”。TH的表达和 PKC酶的调节将通过蛋白质水平的测定来监测， 市售单克隆抗体来探测蛋白质印迹。基因 TH和PKC的表达将通过总的和 使用北方印迹分析进行聚（A）+ RNA分离。 反向- 转录酶-聚合酶链反应将用于检查和 证实了酪氨酸羟化酶表达增强前后 铅治疗。

The long term objective of this project is to develop Dr. R. Renee Reams into an independent biomedical investigator, who will use techniques learned in a selected research mentor's laboratory to generate scientific data the an be used to apply for R01 research funding. The research mentoring will be provided in Dr. Raymond F. Novak's laboratory at the Institute of Chemical Toxicology at Wayne State University. Techniques learned by Dr. Reams will help her define and research problems that will elucidate the mechanism of action of "lead" on mammalian systems. he specific techniques that Dr. Reams will learn in Dr. Novak's laboratory include cell culture of both primary hepatocytes as well as passage and cell lines, western blot analysis, total and poly (A) + RNA isolation, northern blot analysis, nuclear run on assays, reverse-transcriptase polymerase chain reaction, restriction analysis, cloning and DNA sequence analysis. These contemporary approaches in cell and molecular biology will be used by Dr. Reams in the laboratory at Florida A&M University to examine the "Effects of Lead on Tyrosine Hydroxylase Gene Expression and signal Transduction in Adrenal and Neural Cells". Expression of TH and modulation of PKC enzymes will be monitored t the level of protein using a commercially available monoclonal antibody to probe western blots. Gene expression of TH and PKC will be detected by the presence of total and poly (A) + RNA isolation using northern blot analysis. Reverse- transcriptase-polymerase chain reaction will be used to examine and confirm the enhanced expression of tyrosine hydroxylase before and after lead treatment.