Spatiotemporal transcriptomics at the maternal-fetal interface in COVID placenta
新冠肺炎胎盘母胎界面的时空转录组学
基本信息
- 批准号:10618958
- 负责人:
- 金额:$ 21.9万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-05-06 至 2025-04-30
- 项目状态:未结题
- 来源:
- 关键词:2019-nCoVBrainCOVID-19COVID-19 patientCOVID-19 preventionCOVID-19 vaccinationCellsCerebrumClinical ManagementDecidual CellDefense MechanismsEmbryoEpithelial CellsFetal DevelopmentFetal MembranesFetusFormalinFutureGene Expression ProfilingGenesGenomicsGestational DiabetesHomeostasisImmuneImmune systemImmunologicsInfectionInnate Immune SystemInterferon-betaInterferonsInternationalLow Birth Weight InfantMapsMaternal MortalityMaternal-Fetal ExchangeMesenchymalMolecularMolecular ProfilingMorphologyNatural ImmunityNeonatalNucleic AcidsNucleocapsid ProteinsOrganOrganoidsOutcomeParaffin EmbeddingPathway interactionsPatternPlacentaPre-EclampsiaPregnancyPregnant WomenPremature BirthPrevent viral transmissionProductionProteinsRNA VirusesResearchRiskSARS-CoV-2 infectionSARS-CoV-2 spike proteinSignal PathwaySignal Transduction PathwayStimulator of Interferon GenesStromal CellsSymptomsSyncytiotrophoblastSystemTLR7 geneTechniquesTestingTissue EmbeddingTissuesVaccinationVertical TransmissionVillousViralVirus DiseasesWomanWomen&aposs GroupX Chromosomeadverse pregnancy outcomecell typecoronavirus diseasecytokinecytotrophoblastepidemiology studyfetalgene networkglobal healthinsightmaternal morbiditynovelpandemic diseasepathogenpost SARS-CoV-2 infectionpregnantresponsesensorspatiotemporalstillbirthtranscriptometranscriptome sequencingtranscriptomicstransmission processtrophoblastviral transmission
项目摘要
COVID-19, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has emerged as the
greatest threat to global health and significantly impact pregnant women worldwide. COVID-19 infection during
pregnancy is associated with substantial risk of increases in maternal morbidity and mortality and neonatal
complications, compared with their non-infected pregnant counterparts. However, vertical transmission of the
virus is uncommon, which suggest that innate immune system must function at the maternal-fetal interface to
shield the developing fetus from infection. Despite fast-tracked intensive research on many aspects of SARS-
CoV-2, the impact of the viral infection in the placenta and the placental defense mechanism(s) are poorly
explored. Placenta is the structural and immunological barrier of pathogen transmission. To explore the innate
antiviral defense mechanism(s), in our preliminary study we specifically determined expression of interferon
pathway-related molecules in placental tissues. Strikingly, we found distinct patterns of stimulator of interferon
genes (STING), interferon regulator factor 3 (IRF3), and Type I IFN (such as IFNb) expression at the maternal-
fetal interface of the placentas from women infected with COVID-19. STING is a sensor for viral infection.
STING-IRF3 pathway signaling regulates Type I IFN production, and Type I IFNs are potent antiviral cytokines.
We also noticed that Toll-like receptor 7 (TLR7) is upregulated in both syncytiotrophoblasts (
STs) and
extravillous trophoblasts (EVTs) in placentas from patients with COVID-19 compared to those are not. TLR7,
located on the X chromosome, is a sensor for single strand RNA (ssRNA) viruses. These findings are intriguing
and indicate that an innate antiviral immune system is activated at the maternal-fetal interface in patients with
COVID-19 during pregnancy. The objective of the study is to identify spatiotemporal transcriptomic signatures
and networks of the innate antiviral immune system at the maternal-fetal interface. We will test the hypothesis
an effective and robust innate antiviral immune system is activated at the maternal-fetal interface to prevent
viral transmission and shield the fetus from infection, which will be tested in two specific aims.
Aim 1 will
identify spatiotemporal innate immunity gene profiles and networks in villous tissue that respond to maternal
SARS-CoV-2 infection. Aim 2 will identify spatiotemporal innate immunity gene profiles and networks in fetal
membrane and decidual tissue that respond to maternal SARS-CoV-2 infection. A state-of-the-art novel
technique of 10x Genomics Visium Gene Expression assay in placental formalin-fixed paraffin embedded
(FFPE) tissues combined with RNA-seq will be employed
. Placentas from women with active COVID infection,
recovered from COVID infection, and received COVID vaccination, etc. will be studied. This novel ideal
approach will allow us to map the whole transcriptome with morphological context in placental FFPE tissue and
discover the cellular and molecular profiles and networks in different cell types and gene activity at the
maternal-fetal interface without disruption of the tissue integrity. Results of the study will provide novel insights
into how
SARS-CoV-2 infection
impacts the innate immune system at the mater-fetal interface, and how the
innate immune system at the maternal-fetal interface responds to COVID vaccination during pregnancy, which
may help to identify new strategies to prevent COVID-19 infection and reduce maternal and fetal complications
in women with COVID-19 infection in the future.
由严重急性呼吸系统综合征冠状病毒2 (SARS-CoV-2)引起的COVID-19已成为全球最严重的疾病
项目成果
期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Cell-type specific distribution and activation of type I IFN pathway molecules at the placental maternal-fetal interface in response to COVID-19 infection.
细胞类型的特异性分布和I型IFN途径分子在胎盘母亲界面上的激活,响应于19 covid-19的感染。
- DOI:10.3389/fendo.2022.951388
- 发表时间:2022
- 期刊:
- 影响因子:5.2
- 作者:
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YUPING WANG其他文献
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{{ truncateString('YUPING WANG', 18)}}的其他基金
Spatiotemporal transcriptomics at the maternal-fetal interface in COVID placenta
新冠肺炎胎盘母胎界面的时空转录组学
- 批准号:
10440706 - 财政年份:2022
- 资助金额:
$ 21.9万 - 项目类别:
Mechanism of chymase activation in endothelial cells
内皮细胞食糜酶激活机制
- 批准号:
8636206 - 财政年份:2014
- 资助金额:
$ 21.9万 - 项目类别:
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