Consequences of receptor cross talk on inflammation and algesia

受体串扰对炎症和痛觉的影响

• 批准号: 7733163
• 负责人: JOOST J OPPENHEIM
• 金额: $ 7.31万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7733163
• 关键词: Adenosine; Air; Analgesics; Animal Model; Anti-Inflammatory Agents; Anti-inflammatory; Biological Assay; Cancer Vaccines; Capsaicin; Cells; Cyclic AMP-Dependent Protein Kinases; Cytokine Receptors; Exposure to; Goals; Herpes zoster disease; Hormonal; Host resistance; Immunology; Immunosuppressive Agents; In Vitro; Inflammation; Inflammatory; Injection of therapeutic agent; Laboratories; Lesion; Leukocytes; Malignant Neoplasms; Mediating; Mus; Neurons; Neuropeptides; Opioid; Opioid Analgesics; Opioid Receptor; PAG gene; Pain; Pathway interactions; Purinergic P1 Receptors; RANTES; Rattus; Reaction; Receptor Cross-Talk; Reporting; Resolution; Role; Signal Transduction; Spinal Ganglia; Stimulus; TRPV1 gene; Tail; Transactivation; Tumor Biology; Vaccine Adjuvant; Vanilloid; Withdrawal; anandamide; base; capsaicin receptor; chemokine; chemokine receptor; cytokine; desensitization; human PAG protein; in vivo; inflammatory pain; novel; protective effect; receptor; release of sequestered calcium ion into cytoplasm; response; tumor

We established that neurons present in dorsal root ganglia (DRG), similar to leukocytes, express a wide variety of receptors for cytokines, chemokines, opioids, anandamide and other neuropeptides. We previously showed that prior exposure to chemokines such as MIP1alpha results in PKC mediated desensitization of the chemotactic response to opioids by opioid receptors, and thus potentially enhances pain. This decrease in the analgesic effect of opioids was evident from the enhanced tail flick assay of rats administered MIP1alpha or RANTES prior to an analgesic opioid into the PAG of the CNS. We then extended these earlier studies by showing that prior administration of chemokines Asensitized and primed the calcium flux of capsaicin or anandamide stimulated vanilloid (TRPV1) algesic receptor on DRG neurons. This response also increased pain as shown by the enhancement of paw withdrawal in response to the intrathecal administration of the chemokine prior to capsaicin in vivo. This sensitization of the vanilloid receptor was also PKC dependent. Consequently, proinflammatory chemokines can increase pain both by suppressing opioid and enhancing vanilloid receptor responses. Based on these studies, we predicted that the anti-inflammatory effects of adenosine, which also interacts with GiPCR, might have effects on chemokine receptors. Indeed our current studies show that prior addition of adenosine results in suppressing the in vitro chemotactic response of leukocytes to a variety of chemokines. Furthermore, prior in vivo injection of adenosine inhibits the in vivo influx of leukocytes into a murine air pouch by about 90%. This cross-desensitization of chemokine receptors by adenosine A2a receptors was PKA dependent. These studies therefore reveal novel pathways of receptor mediated intercommunication of inflammatory as well as painful stimuli. Means of interfering with these PKC and PKA dependent signals and the pathophysiological relevance of this receptor cross-talk to inflammation and pain need to be further evaluated. We are currently investigating how these pathways may be contributing to the very painful inflammatory lesions of Herpes Zoster in animal models. The role of adenosine as an immunosuppressive effector molecule also has been reported to mediate the cell contact dependent effects of Tregs and to interfere with host resistance to tumors. Thus, studies of adenosine effects are relevant to tumor biology and immunology. The projects of my laboratory are focused on the resolution and control of inflammation and cancer, to achieve this goal we have taken three diverse approaches involving studies of the cross-talk between pain and inflammatory receptors, the tumor protective effects of T regulatory cells and the identification of alarmins that may prove useful as anti tumor vaccine adjuvants.

我们发现，存在于背根神经节(DRG)的神经元，类似于白细胞，表达多种细胞因子、趋化因子、阿片类物质、去雄激素和其他神经肽的受体。我们以前曾证明，预先暴露于趋化因子如MIP1α会导致PKC介导的阿片受体对阿片类药物的趋化反应脱敏，从而潜在地增强疼痛。在阿片类药物进入中枢PAG之前，给予MIP1α或RANTES大鼠的增强甩尾实验表明，阿片类药物的镇痛作用明显减弱。然后，我们扩展了这些早期的研究，表明预先给予趋化因子钝化和启动辣椒素或双胺刺激的DRG神经元上的香草素(TRPV1)痛性受体的钙流。这种反应也增加了疼痛，表现为在体内鞘内注射辣椒素之前给予趋化因子后，爪子缩回的增强。香草素受体的这种敏化也依赖于PKC。因此，促炎趋化因子可以通过抑制阿片类药物和增强香草素受体反应来增加疼痛。在这些研究的基础上，我们预测腺苷的抗炎作用可能会影响趋化因子受体，腺苷也与GiPCR相互作用。事实上，我们目前的研究表明，预先加入腺苷会抑制白细胞对各种趋化因子的体外趋化反应。此外，预先体内注射腺苷可抑制体内白细胞流入小鼠气囊约90%。腺苷A2a受体对趋化因子受体的这种交叉脱敏作用依赖于PKA。因此，这些研究揭示了受体介导的炎性和疼痛刺激相互交流的新途径。干扰这些PKC和PKA依赖信号的方法以及这种受体串扰与炎症和疼痛的病理生理学相关性需要进一步评估。我们目前正在研究这些途径是如何在动物模型中导致带状疱疹非常痛苦的炎症损害的。腺苷作为免疫抑制效应分子的作用也已被报道，以介导Tregs的细胞接触依赖效应，并干扰宿主对肿瘤的抵抗。因此，腺苷作用的研究与肿瘤生物学和免疫学有关。我的实验室的项目集中在炎症和癌症的消解和控制上，为了实现这一目标，我们采取了三种不同的方法，包括研究疼痛和炎症受体之间的串扰，T调节细胞的肿瘤保护作用，以及鉴定可能被证明是有用的抗肿瘤疫苗佐剂的警示蛋白。