Functionally Closed Purification and Elution of Untouched Cells using Cleavable Magnetic Beads with Digital Magnetic Sorting

项目摘要 分离特定和明确定义的细胞类型以产生下一代细胞疗法仍然是一个显著的规模扩大 并扩大自体和同种异体治疗的挑战。 通过对细胞治疗开发人员的主要客户访谈，我们了解到可扩展细胞分选的需求尚未得到满足 该系统可以在生产细微差别的细胞治疗剂中分离多种标记物上的稀有细胞类型。具有初步 研究，我们证明了我们的可扩展核心技术（数字磁分选）实现了上级稀有细胞分离 并且可以实现并行化的多目标分类以满足这种需要。 在这项提案中，我们的目标是开发一种功能封闭的定量细胞纯化系统，可以分离罕见的， 基于多种标记物的特定细胞类型，以有利于细胞疗法制造的生产量。

Project Summary Isolation of specific and well-defined cell types to produce next generation cellular therapies remains a significant scale up and scale out challenge for autologous and allogeneic therapies. Through primary customer interviews of cell therapy developers, we learned there is an unmet need for scalable cell sorting systems that can isolate rare cell types on multiple markers in production of nuanced cellular therapeutics. With preliminary studies, we demonstrated that our scalable core technology (digital magnetic sorting) achieves superior rare cell isolation and can achieve parallelized multitarget sorting to address this need. In this proposal, we aim to develop a functionally closed, quantitative cell purification system that can isolate rare and specific cell types based on multiple markers at throughputs conducive for cell therapy manufacturing.