Functionally Closed Purification and Elution of Untouched Cells using Cleavable Magnetic Beads with Digital Magnetic Sorting

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Project Summary Isolation of specific and well-defined cell types to produce next generation cellular therapies remains a significant scale up and scale out challenge for autologous and allogeneic therapies. Through primary customer interviews of cell therapy developers, we learned there is an unmet need for scalable cell sorting systems that can isolate rare cell types on multiple markers in production of nuanced cellular therapeutics. With preliminary studies, we demonstrated that our scalable core technology (digital magnetic sorting) achieves superior rare cell isolation and can achieve parallelized multitarget sorting to address this need. In this proposal, we aim to develop a functionally closed, quantitative cell purification system that can isolate rare and specific cell types based on multiple markers at throughputs conducive for cell therapy manufacturing.