Functionally Closed Purification and Elution of Untouched Cells using Cleavable Magnetic Beads with Digital Magnetic Sorting

项目摘要 分离特定和定义明确的细胞类型以产生下一代细胞疗法仍然是一个重要的规模 并横向扩展自体和同种异体疗法的挑战。 通过对细胞疗法开发商的主要客户采访，我们了解到对可伸缩细胞分类的需求尚未得到满足 在细微差别细胞疗法的生产中，可以在多个标记上分离稀有细胞类型的系统。具有初步版本 通过研究，我们证明了我们的可扩展核心技术(数字磁选)实现了卓越的稀有细胞分离 并且可以实现并行多目标排序，以满足这一需求。 在这个方案中，我们的目标是开发一种功能封闭的定量细胞纯化系统，该系统可以分离稀有和 基于多个标记的特定细胞类型的生产量有利于细胞疗法的制造。

Project Summary Isolation of specific and well-defined cell types to produce next generation cellular therapies remains a significant scale up and scale out challenge for autologous and allogeneic therapies. Through primary customer interviews of cell therapy developers, we learned there is an unmet need for scalable cell sorting systems that can isolate rare cell types on multiple markers in production of nuanced cellular therapeutics. With preliminary studies, we demonstrated that our scalable core technology (digital magnetic sorting) achieves superior rare cell isolation and can achieve parallelized multitarget sorting to address this need. In this proposal, we aim to develop a functionally closed, quantitative cell purification system that can isolate rare and specific cell types based on multiple markers at throughputs conducive for cell therapy manufacturing.