Customer Discovery for Ferrologix Digital Magnetic Sorting

Ferrologix 数字磁力分选的客户发现

• 批准号: 10044395
• 负责人: Coleman Murray
• 金额: $ 5.46万
• 依托单位: FERROLOGIX, INC.
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-08-20 至 2022-08-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10044395
• 关键词: Architecture; Automation; Cell Separation; Cell secretion; Cells; Clinical; Complex; Cytometry; Disease; Enzyme-Linked Immunosorbent Assay; Equilibrium; Goals; Immune System Diseases; Immunologics; Immunotherapy; Individual; Interview; Magnetism; Market Research; Measurement; Measures; Medical; Methods; Microfluidics; Monitor; Phase; Phenotype; Research Personnel; Resolution; Sorting - Cell Movement; TNF gene; Techniques; Technology; Voice; Work; clinical translation; cost; digital; high throughput screening; monocyte; research and development; single cell analysis; targeted treatment; tool

Project Summary Treatment of immunological disorders with emerging treatments such as immunotherapy represent a growing medical paradigm. To create potent and targeted therapies, defining the underlying immunological mechanisms of action and is critical. Because of immunological complexity, measurements with single cell resolution are essential as both diseases and treatments are often driven by small subpopulations of cells. However, due to limitations with current cell isolation and analysis tools, precisely mapping these complex interactions with single cell resolution is difficult and cost prohibitive to deploy at a scale conducive for high throughput target discovery or streamlined clinical monitoring. Most high precision single cell isolation and analysis tools, such as FACS or microfluidic platforms, are too low throughput and are difficult to integrate into SBS formats for high throughput screening (HTS) workflows. Contrastingly, bulk cell sorting methods such as MACS are easily integrated into HTS formats but cannot achieve single cell capture or analysis. The inherent tradeoff of single cell precision and scalability impeads immunological R&D and clinical translation efforts. Through primary market research interviews using I Corp techniques, researchers have voiced their dissatisfaction with the current single cell isolation tools saying they are a “major roadblock in our work on phenotypic analysis.” We reasoned that a product that can integrate cell isolation and single cell analysis in an SBS well plate format would alleviate these bottlenecks and accelerate clinical translation. Leveraging a core technology known as ratcheting cytometry, the objective of this proposal to develop a “smart plate” which can achieve integrated cell purification and arrangement into individual cells for single cell interrogation in SBS format. In preliminary studies, ratcheting cytometry has demonstrated quantitative cell sorting capacity and capacity to self-assemble cells into a single cell format. In phase I, ratcheting cytometry chips will be used to (1) purify CD 14+ monocytes into individual cells followed by (2) co-localization of commercially available magnetic ELISA beads to measure TNF α secretion activity of single cells. Phase II will focus on scaling the ratcheting cytometry cartridges and integration into SBS well plate format for HTS workflows.

项目概要 使用免疫疗法等新兴疗法治疗免疫性疾病代表着日益增长的趋势 医学范式。创造有效的靶向疗法，定义潜在的免疫学 作用机制并且至关重要。由于免疫学的复杂性，用单细胞进行测量 分辨率至关重要，因为疾病和治疗通常都是由小细胞亚群驱动的。 然而，由于当前细胞分离和分析工具的限制，精确映射这些复杂的 与单细胞分辨率的相互作用是困难的，并且以有利于高水平的规模部署成本高昂 吞吐量目标发现或简化的临床监测。最高精度的单细胞分离和 分析工具，例如 FACS 或微流控平台，通量太低并且难以集成到 适用于高通量筛选 (HTS) 工作流程的 SBS 格式。相比之下，批量细胞分选方法，例如 MACS 很容易集成到 HTS 格式中，但无法实现单细胞捕获或分析。固有的 单细胞精度和可扩展性的权衡阻碍了免疫研发和临床转化工作。 通过使用 I Corp 技术进行的一级市场研究访谈，研究人员表达了他们的看法 对当前单细胞分离工具的不满，称它们是“我们工作中的主要障碍” 表型分析。” 我们推断出一种可以在 SBS 孔板格式中集成细胞分离和单细胞分析的产品 将缓解这些瓶颈并加速临床转化。利用称为 棘轮细胞术，本提案的目标是开发一种可以实现细胞整合的“智能板” 纯化并排列成单个细胞，以 SBS 格式进行单细胞询问。在初步 研究表明，棘轮细胞术已经证明了定量细胞分选能力和自组装能力 将单元格转换为单个单元格格式。在第一阶段，棘轮细胞计数芯片将用于 (1) 纯化 CD 14+ 将单核细胞转化为单个细胞，然后 (2) 将市售的 ELISA 磁珠共定位 测定单细胞TNFα分泌活性。第二阶段将重点关注棘轮细胞术的扩展 卡盒并集成到 SBS 孔板格式中，用于 HTS 工作流程。