Customer Discovery for Ferrologix Digital Magnetic Sorting

项目摘要 用新兴疗法如免疫疗法治疗免疫性病症代表了日益增长的免疫学治疗。 医学范式创造有效的靶向治疗，确定潜在的免疫学 行动机制，至关重要。由于免疫学的复杂性， 解决方案是必不可少的，因为疾病和治疗往往是由小的细胞亚群驱动的。 然而，由于当前细胞分离和分析工具的局限性，精确地映射这些复杂的细胞是不可能的。 与单小区分辨率的交互是困难的，并且成本过高， 通量目标发现或简化的临床监测。最高精度的单细胞分离和 例如FACS或微流体平台的分析工具的通量太低，并且难以集成到其中。 SBS格式用于高通量筛选（HTS）工作流程。相比之下，批量细胞分选方法，如 MACS很容易集成到HTS格式中，但无法实现单细胞捕获或分析。固有的 单细胞精确度和可扩展性的折衷阻碍了免疫学研发和临床转化工作。 通过使用I Corp技术进行的主要市场研究访谈，研究人员表达了他们的观点。 他对目前的单细胞分离工具表示不满，称它们是“我们研究的主要障碍”。 表型分析”。 我们推断，可以在SBS孔板格式中集成细胞分离和单细胞分析的产品 将缓解这些瓶颈并加速临床转化。利用核心技术， 棘轮细胞仪，本建议的目的是开发一种“智能板”，可以实现集成细胞 纯化并排列成单个细胞，用于SBS格式的单细胞询问。初步 研究表明，棘轮细胞仪已经证明了定量细胞分选能力和自组装能力， 单元格转换为单个单元格格式。在第一阶段，棘轮细胞仪芯片将用于（1）纯化CD 14+ 单核细胞进入单个细胞，然后（2）将市售的磁性ELISA珠共定位 测定单个细胞的TNF α分泌活性。第二阶段的重点是扩大棘轮细胞仪的规模 检测盒和集成到SBS孔板格式的HTS工作流程。

Project Summary Treatment of immunological disorders with emerging treatments such as immunotherapy represent a growing medical paradigm. To create potent and targeted therapies, defining the underlying immunological mechanisms of action and is critical. Because of immunological complexity, measurements with single cell resolution are essential as both diseases and treatments are often driven by small subpopulations of cells. However, due to limitations with current cell isolation and analysis tools, precisely mapping these complex interactions with single cell resolution is difficult and cost prohibitive to deploy at a scale conducive for high throughput target discovery or streamlined clinical monitoring. Most high precision single cell isolation and analysis tools, such as FACS or microfluidic platforms, are too low throughput and are difficult to integrate into SBS formats for high throughput screening (HTS) workflows. Contrastingly, bulk cell sorting methods such as MACS are easily integrated into HTS formats but cannot achieve single cell capture or analysis. The inherent tradeoff of single cell precision and scalability impeads immunological R&D and clinical translation efforts. Through primary market research interviews using I Corp techniques, researchers have voiced their dissatisfaction with the current single cell isolation tools saying they are a “major roadblock in our work on phenotypic analysis.” We reasoned that a product that can integrate cell isolation and single cell analysis in an SBS well plate format would alleviate these bottlenecks and accelerate clinical translation. Leveraging a core technology known as ratcheting cytometry, the objective of this proposal to develop a “smart plate” which can achieve integrated cell purification and arrangement into individual cells for single cell interrogation in SBS format. In preliminary studies, ratcheting cytometry has demonstrated quantitative cell sorting capacity and capacity to self-assemble cells into a single cell format. In phase I, ratcheting cytometry chips will be used to (1) purify CD 14+ monocytes into individual cells followed by (2) co-localization of commercially available magnetic ELISA beads to measure TNF α secretion activity of single cells. Phase II will focus on scaling the ratcheting cytometry cartridges and integration into SBS well plate format for HTS workflows.