Development of MRI Techniques for Drug-Abuse Applications

药物滥用领域 MRI 技术的发展

• 批准号: 7733806
• 负责人: Yihong Yang
• 金额: $ 301.47万
• 依托单位: NATIONAL INSTITUTE ON DRUG ABUSE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7733806
• 关键词: Acute; Adult; Algorithms; Aminobutyric Acid; Aminobutyric Acids; Amygdaloid structure; Anesthetics; Animal Model; Animals; Back; Base of the Brain; Bilateral; Blood - brain barrier anatomy; Bolus Infusion; Brain; Brain region; Characteristics; Chloralose; Cocaine; Computer software; Computers; Condition; Coupled; Custom; Data; Dependence; Detection; Development; Diffusion; Diffusion Magnetic Resonance Imaging; Disruption; Dose; Drug Addiction; Echo-Planar Imaging; Electroencephalography; Equation; Evolution; Fiber; Frequencies; Functional Magnetic Resonance Imaging; Functional disorder; Glutamates; Glutamine; Hippocampus (Brain); Human; Image; Imaging Techniques; Injection of therapeutic agent; Intraperitoneal Injections; Localized; MRI Scans; Magnetic Resonance Imaging; Magnetic Resonance Spectroscopy; Manganese; Mannitol; Maps; Measurement; Measures; Metabolic; Methods; Motor; Neurons; Neurotransmitters; Outcome; Patients; Pentylenetetrazole; Pharmaceutical Preparations; Physiologic pulse; Physiological; Principal Component Analysis; Procedures; Process; Protons; Pulse taking; Range; Rate; Rattus; Recovery; Research Project Grants; Resolution; Rest; Running; Scanning; Seizures; Sensory; Series; Signal Transduction; Site; Slice; Solutions; Somatosensory Cortex; Specific qualifier value; Specificity; Spectrum Analysis; Standards of Weights and Measures; Steam; System; Techniques; Time; Tissues; Triplet Multiple Birth; Visual; Visual Cortex; Wing; animal data; base; brain research; day; density; frontal lobe; gamma-Aminobutyric Acid; gray matter; hemodynamics; human subject; image registration; improved; in vivo; independent component analysis; multiplet; neuroimaging; novel; reconstruction; research study; response; simulation; spatiotemporal; success

1. Frequency Specificity of Functional Connectivity in Brain Networks Synchronized low-frequency spontaneous fluctuations of the functional MRI (fMRI) signal have been shown to be associated with electroencephalography (EEG) power fluctuations in multiple brain networks within predefined frequency bands. However, it remains unclear whether frequency-specific characteristics exist in the resting-state fMRI signal. In this study, fMRI signals in five functional brain networks (sensorimotor, edefault modef, visual, amygdala, and hippocampus) were decomposed into various frequency bands within a low frequency range (0-0.24 Hz). Results show that the correlations in cortical networks concentrate within ultra-low frequencies (0.01-0.06 Hz) while connections within limbic networks distribute over a wider frequency range (0.01-0.14 Hz), suggesting distinct frequency-specific features in the resting-state fMRI signal within these functional networks. (Neuroimage. 2008 Sep 1;42(3):1047-55) 2. Group Independent Component Analysis Reveals Consistent Resting-State Networks across Multiple Sessions Group independent component analysis (gICA) was performed on resting-state data from 14 healthy subjects scanned on 5 fMRI scan sessions across 16 days. The data were reduced and aggregated in 3 steps using Principal Components Analysis (PCA, within scan, within session and across session) and subjected to gICA procedures. Analyses were performed using all sessions in order to maximize sensitivity and alleviate the problem of component identification across session. Across-session consistency was examined by three methods, all using back-reconstruction of the single session or single subject/session maps from the grand (5 session) maps. The gICA analysis produced 55 spatially independent maps. Biologically relevant component maps were found, including sensory, motor and a 'default-mode' map. All analysis methods showed that components were remarkably consistent across session. Critically, the components with the most obvious physiological relevance were the most consistent. (Brain Research, in press) 3. Mapping spatiotemporal diffusion inside the human brain using a numerical solution of the diffusion equation We demonstrate the feasibility to reveal the spatiotemporal diffusion process inside the human brain based on a numerical solution of the diffusion equation. Normal human subjects were scanned with a diffusion tensor imaging (DTI) technique on a 3-T MRI scanner, and the diffusion tensor in each voxel was calculated from the DTI data. The diffusion equation was discretized into equivalent algebraic equations. A finite-difference method was employed to obtain the numerical solution of the diffusion equation. By specifying boundary and initial conditions, the spatiotemporal evolution of the diffusion process inside the brain can be virtually reconstructed. The proposed method highlights the feasibility to noninvasively estimate the macroscopic diffusive transport time for a molecule in a given region of the brain. (Magn Reson Imaging. 2008 Jun;26(5):694-702) 4. Spectral simplification for resolved glutamate and glutamine measurement using a standard STEAM sequence with optimized timing parameters at various field strengths A spectral simplification approach is introduced that provides unobstructed Glu and Gln measurement using a standard STEAM localization sequence with optimized interpulse timings. The underlying idea is to exploit the dependence of response of a coupled spin system on the echo time (TE) and mixing time (TM) to find an optimum timing set (TE, TM), at which the outer-wings of C4 "pseudo-triplet" proton resonances of Glu and Gln are significantly suppressed while the central peaks are maintained. Density matrix simulation for Glu, Gln, and other overlapping metabolites at 2.3-2.5 ppm was conducted to predict the optimum timing sets. The simulation, phantom, and in vivo results demonstrated that the C4 multiplet proton resonances of Glu and Gln can be resolved for unobstructed detection at 3T, 4T, and 4.7T. (Magn Reson Med. 2008 Feb;59(2):236-44) 5. Synchronized delta oscillations correlate with the resting-state functional MRI signal Electrophysiological recordings and resting-state fMRI measurements were conducted in alpha-chloralose-anesthetized rats. Region-specific, anesthetic dose-dependent fMRI resting-state functional connectivity was detected in bilateral primary somatosensory cortex (S1FL) of the resting brain. Cortical electroencephalographic signals were also recorded from bilateral S1FL; a visual cortex locus served as a control site. Results demonstrate that the resting-state fMRI signal correlates with the power coherence in low-frequency bands, particularly the delta band. These data indicate that hemodynamic fMRI signal differentially registers specific electrical oscillatory frequency band activity, suggesting that fMRI may be able to distinguish the ongoing from the evoked activity of the brain. (Proc Natl Acad Sci U S A. 2007 Nov 13;104(46):18265-9) 6. Real-time animal functional magnetic resonance imaging and its application to neuropharmacological studies In the present study, an approach for real-time MRI on a Bruker scanner is presented. The custom software runs on the console computer in parallel with the scanner imaging software, and no additional hardware is required. The utility of this technique is demonstrated in manganese-enhanced MRI (MEMRI) with acute cocaine challenge, in which temporary disruption of the blood-brain barrier (BBB) is a critical step for MEMRI experiments. With the aid of real-time MRI, we were able to assess the outcome of BBB disruption following bolus injection of hyperosmolar mannitol in a near real-time fashion prior to drug administration, improving experimental success rate. (Magn Reson Imaging. In press) 7. Fast T1 mapping technique A fast T1 measurement sequence using inversion recovery Look-Locker echo-planar imaging at a steady state (IR LL-EPI SS) has been developed. Imaging parameters of the the sequence were optimized to minimize the bias from excitation pulse imperfections and to maximize the accuracy and reliability of T1 measurements, which are critical for its applications. Compared to conventional inversion recovery Look-Locker echo-planar imaging (IR LL-EPI) sequence, our new sequence method preserves similar accuracy and reliability, while saving 20% in acquisition time. Optimized IR LL-EPI SS provided quantitative T1 mapping with 114 mm3 resolution and whole brain coverage (28 slices) in approximately 4 minutes. (Magn Reson Med. In review) 8. Metabolic Changes in Rat Frontal Cortex after Injection of Pentylenetetrazole Measured by Proton MR Spectroscopy at 9.4T Seizures were induced in rats by intraperitoneal injection of pentylenetetrazole (PTZ, 70 mg/kg). The time series of metabolite concentrations in the frontal cortex of adult rat brain were measured in vivo before and after the injection by localized 1H MRS spectroscopy at 9.4T (3 time points before the injection and 4 time points after, 25 minutes for each time point). Compared to baseline levels, the average concentrations of glutamine (Gln) and -aminobutyric acid (GABA) (n = 7) significantly increased post injection but reached peaks at different time. 9. Image Registration Technique An implicit reference group image registration algorithm has been developed, which can significantly improve the spatial normalization of a group of images. Assessment of the new technique on anatomical MRI images, diffusion tensor images, and functional MRI showed that this method produces more accurate group-based statistical analysis.

1. 大脑网络功能连接的频率特异性 功能性 MRI (fMRI) 信号的同步低频自发波动已被证明与预定义频带内多个大脑网络中的脑电图 (EEG) 功率波动相关。然而，目前尚不清楚静息态 fMRI 信号中是否存在频率特异性特征。在这项研究中，五个功能性大脑网络（感觉运动、默认模式、视觉、杏仁核和海马体）的功能磁共振成像信号被分解为低频范围（0-0.24 Hz）内的各个频段。结果表明，皮质网络中的相关性集中在超低频（0.01-0.06 Hz）内，而边缘网络内的连接分布在更宽的频率范围（0.01-0.14 Hz）内，这表明这些功能网络内静息态 fMRI 信号具有明显的频率特定特征。 （神经影像。2008 年 9 月 1 日；42(3):1047-55） 2. 组独立成分分析揭示了多个会话中一致的静息态网络 对 14 名健康受试者在 16 天内通过 5 次 fMRI 扫描扫描的静息态数据进行了组独立成分分析 (gICA)。使用主成分分析（PCA、扫描内、会话内和跨会话）分 3 个步骤减少和汇总数据，并接受 gICA 程序。使用所有会话进行分析，以便最大限度地提高灵敏度并减轻跨会话的组件识别问题。通过三种方法检查跨会话一致性，所有方法都使用来自大（5 个会话）图的单个会话或单个主题/会话图的反向重建。 gICA 分析生成了 55 个空间独立的地图。发现了生物学相关的成分图，包括感觉、运动和“默认模式”图。所有分析方法均表明各个会话中的成分非常一致。至关重要的是，具有最明显生理相关性的成分是最一致的。 （大脑研究，待出版） 3. 使用扩散方程的数值解来绘制人脑内部的时空扩散 我们证明了基于扩散方程的数值解来揭示人脑内部时空扩散过程的可行性。在 3-T MRI 扫描仪上使用扩散张量成像 (DTI) 技术对正常人类受试者进行扫描，并根据 DTI 数据计算每个体素中的扩散张量。扩散方程被离散化为等效代数方程。采用有限差分法获得扩散方程的数值解。通过指定边界和初始条件，可以虚拟地重建大脑内扩散过程的时空演化。所提出的方法强调了无创地估计大脑给定区域中分子的宏观扩散传输时间的可行性。 （磁共振成像。2008 年 6 月；26(5):694-702） 4. 使用标准 STEAM 序列对解析的谷氨酸和谷氨酰胺测量进行光谱简化，并在不同场强下优化定时参数 引入了光谱简化方法，该方法使用具有优化脉冲间时序的标准 STEAM 定位序列来提供无障碍的 Glu 和 Gln 测量。基本思想是利用耦合自旋系统响应对回波时间（TE）和混合时间（TM）的依赖性来找到最佳定时集（TE，TM），在该定时集上，Glu和Gln的C4“伪三重态”质子共振的外翼被显着抑制，同时保持中心峰。对 2.3-2.5 ppm 的 Glu、Gln 和其他重叠代谢物进行密度矩阵模拟，以预测最佳时间设置。模拟、模型和体内结果表明，Glu 和 Gln 的 C4 多重质子共振可以在 3T、4T 和 4.7T 下进行无障碍检测。 （Magn Reson Med. 2008 年 2 月；59(2):236-44） 5. 同步 delta 振荡与静息态功能 MRI 信号相关 在α-氯醛糖麻醉的大鼠中进行电生理记录和静息态功能磁共振成像测量。在静息大脑的双侧初级体感皮层（S1FL）中检测到区域特异性、麻醉剂量依赖性功能磁共振成像静息态功能连接。还记录了双侧 S1FL 的皮质脑电图信号；视觉皮层位点作为对照位点。结果表明，静息态 fMRI 信号与低频段（尤其是 delta 频段）的功率相干性相关。这些数据表明，血流动力学功能磁共振成像信号有差异地记录了特定的电振荡频带活动，这表明功能磁共振成像可能能够区分大脑正在进行的活动和诱发的活动。 （美国国家科学院院报，2007 年 11 月 13 日；104(46):18265-9） 6. 实时动物功能磁共振成像及其在神经药理学研究中的应用 在本研究中，提出了一种在布鲁克扫描仪上进行实时 MRI 的方法。定制软件在控制台计算机上与扫描仪成像软件并行运行，不需要额外的硬件。该技术的实用性在可卡因急性激发的锰增强 MRI (MEMRI) 中得到了证明，其中血脑屏障 (BBB) 的暂时破坏是 MEMRI 实验的关键步骤。借助实时 MRI，我们能够在给药前以近乎实时的方式评估推注高渗甘露醇后 BBB 破坏的结果，从而提高实验成功率。 （磁共振成像。正在印刷中） 7. 快速T1映射技术 已经开发出一种使用稳定状态下反转恢复 Look-Locker 回波平面成像 (IR LL-EPI SS) 的快速 T1 测量序列。该序列的成像参数经过优化，可最大限度地减少激励脉冲缺陷带来的偏差，并最大限度地提高 T1 测量的准确性和可靠性，这对其应用至关重要。与传统的反演恢复 Look-Locker 回波平面成像 (IR LL-EPI) 序列相比，我们的新序列方法保留了类似的准确性和可靠性，同时节省了 20% 的采集时间。优化的 IR LL-EPI SS 在大约 4 分钟内提供分辨率为 114 mm3 的定量 T1 映射和全脑覆盖（28 个切片）。 （Magn Reson Med. 评论中） 8. 9.4T 质子磁共振波谱测量注射戊四唑后大鼠额叶皮质的代谢变化 通过腹腔注射戊四唑（PTZ，70 mg/kg）诱导大鼠癫痫发作。采用9.4T的局部1H MRS波谱在体内测量成年大鼠大脑额叶皮层代谢物浓度的时间序列（注射前3个时间点，注射后4个时间点，每个时间点25分钟）。与基线水平相比，注射后谷氨酰胺（Gln）和γ-氨基丁酸（GABA）（n = 7）的平均浓度显着增加，但在不同时间达到峰值。 9. 图像配准技术 开发了一种隐式参考组图像配准算法，可以显着提高一组图像的空间标准化。对解剖 MRI 图像、扩散张量图像和功能 MRI 新技术的评估表明，该方法可以产生更准确的基于组的统计分析。

项目成果

A rotation-invariant spherical harmonic decomposition method for mapping intravoxel multiple fiber structures.

用于映射体素内多纤维结构的旋转不变球谐分解方法。

• DOI: 10.1016/j.neuroimage.2005.08.045
• 发表时间: 2006
• 期刊: NeuroImage
• 影响因子: 5.7
• 作者: Zhan,Wang;Stein,ElliotA;Yang,Yihong
• 通讯作者: Yang,Yihong

Simultaneous perfusion and blood-oxygenation-level-dependent measurements using single-shot interleaved z-shim echo-planar imaging.

使用单次交错 z 垫片回波平面成像同时进行灌注和血氧水平依赖性测量。

• DOI: 10.1002/mrm.20431
• 发表时间: 2005
• 期刊: Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine
• 作者: Yang,Yihong;Gu,Hong;Silbersweig,DavidA;Stern,Emily
• 通讯作者: Stern,Emily

Mapping spatiotemporal diffusion inside the human brain using a numerical solution of the diffusion equation.

使用扩散方程的数值解来绘制人脑内部的时空扩散。

• DOI: 10.1016/j.mri.2008.01.025
• 发表时间: 2008
• 期刊: Magnetic resonance imaging
• 影响因子: 2.5
• 作者: Zhan,Wang;Jiang,Li;Loew,MurrayH;Yang,Yihong
• 通讯作者: Yang,Yihong

Frequency specificity of functional connectivity in brain networks.

• DOI: 10.1016/j.neuroimage.2008.05.035
• 发表时间: 2008-09-01
• 期刊: NEUROIMAGE
• 影响因子: 5.7
• 作者: Wu, Changwei W.;Gu, Hong;Lu, Hanbing;Stein, Elliot A.;Chen, Jyh-Horng;Yang, Yihong
• 通讯作者: Yang, Yihong

Group independent component analysis reveals consistent resting-state networks across multiple sessions.

• DOI: 10.1016/j.brainres.2008.08.028
• 发表时间: 2008-11-06
• 期刊: Brain research
• 影响因子: 2.9
• 作者: Chen S;Ross TJ;Zhan W;Myers CS;Chuang KS;Heishman SJ;Stein EA;Yang Y
• 通讯作者: Yang Y