DNA Polymerase Database

项目描述：该项目涉及DNA聚合酶综合数据库- DNAPolBase的设计、构建和出版。DNAPolBase将作为DNA聚合酶生物化学，生物学，遗传学和结构的免费提供的高质量资源。该项目的工地将位于马萨诸塞州伊普斯威奇的新英格兰生物实验室。DNA复制的破坏是几乎所有遗传疾病状态的原因，而负责这一复制过程的核心机器是DNA聚合酶。DNA聚合酶也是多种诊断和分子生物学应用中的关键元件，包括DNA测序和聚合酶链式反应（PCR）。基于聚合酶的应用经常涉及非天然底物和反应条件，并且越来越多地寻求非常适合于这些应用的工程化酶。新的聚合酶和整个新的超级家族的鉴定激发了人们对这些酶的新兴趣。这种丰富的信息使得休闲和经验丰富的研究人员越来越难以保持对该领域的全面了解。尽管它们的重要性，没有一个单一的储存库的信息有关的研究和使用的DNA聚合酶目前存在。我们将从公共和私人记录中编译丰富的已知聚合酶数据到一个可搜索的数据库中，为了解这些酶在体内的作用提供有用的第一步，并为它们在众多应用中的使用和适应提供信息。作为数据库开发基础工作的一部分，已经开始与愿意用自己的私人和公共聚合酶数据和记录库为该数据库提供种子的科学家进行关键合作。从这个吉祥的开始，将组织一个数据库，以满足众多用户群体的信息需求，包括基础和制药研究人员，应用为基础的科学家，教师和学生。将提供从大规模比较研究（希望推进基于聚合酶的研究和应用的许多方面）到聚合酶性质或起源的简单查询的功能。我们预计NEB将以与公众相同的方式受益于获得这种资源，即通过推进我们的内部DNA聚合酶开发项目。公共卫生相关性：DNA聚合酶是复制所有生物的遗传蓝图的代理人，其功能的腐败是几乎所有遗传疾病状态的原因。此外，DNA聚合酶还被选为关键诊断平台的引擎，特别是基于DNA的检测系统和DNA测序。一个关于DNA聚合酶的信息的中央储存库将是理解和应对错误DNA复制的影响的重要资源，同时还将促进更快速、有效和具有成本效益的诊断平台的开发。

DESCRIPTION (provided by applicant): This project concerns the design, construction and publication of a comprehensive database on DNA polymerases - DNAPolBase. DNAPolBase will serve as a freely available, high quality resource of DNA polymerase biochemistry, biology, genetics and structure. The worksite for this project will be New England Biolabs, in Ipswich, MA. The corruption of DNA copying is responsible for virtually all genetic disease states and the core machines responsible for this copying process are DNA polymerases. DNA polymerases are also key elements in a variety of diagnostic and molecular biology applications including DNA sequencing and polymerase chain reaction (PCR). Polymerase-based applications frequently involve non-natural substrates and reaction conditions, and engineered enzymes well suited to these applications are increasingly sought. The identification of novel polymerases and entire new super families has spurred renewed interest in these enzymes. This abundance of information makes it increasingly difficult for casual and experienced researchers alike to maintain a comprehensive view of the field. Despite their importance, no single repository for information relevant to the study and use of DNA polymerases currently exists. We will compile the wealth of known polymerase data from public and private records into a searchable database to provide a useful first step in understanding the role of these enzymes in vivo, and to inform their use in and adaptation to numerous applications. As part of the groundwork of database development, key collaborations with scientists willing to seed this database with their own libraries of private and public polymerase data and records have begun. From this auspicious start a database will be organized to serve the informational needs of numerous user groups, including basic and pharmaceutical researchers, application based scientists, instructors, and students. Functions from large-scale comparative studies (desired to advance many aspects of polymerase-based research and applications) to simple queries of polymerase properties or origins will be provided. We anticipate that NEB will benefit in much the same way that the public will by having access to such a resource, namely by advancing our internal DNA polymerase development projects. PUBLIC HEALTH RELEVANCE: DNA Polymerase Database Project Narrative DNA polymerases are the agents that copy the genetic blueprint of all living creatures, and corruption of their functions is the cause of virtually all genetic disease states. DNA polymerases have additionally been co-opted to serve as the engines of key diagnostic platforms, notably DNA-based detection systems and DNA sequencing. A central repository for information on DNA polymerases will be an important resource in understanding and coping with the effects of errant DNA copying, while additionally enhancing development of more rapid, effective, and cost-effective diagnostic platforms.