DNA Polymerase Database

DNA聚合酶数据库

• 批准号: 7608537
• 负责人: WILLIAM E JACK
• 金额: $ 10.7万
• 依托单位: NEW ENGLAND BIOLABS, INC.
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-03-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7608537
• 关键词: Academia; Biochemistry; Biology; Characteristics; Collaborations; Collection; Comparative Study; Copying Processes; DNA; DNA Sequence; DNA-Directed DNA Polymerase; Data; Data Quality; Data Set; Databases; Detection; Development; Diagnostic; Engineering; Ensure; Enzymes; Family; Genetic; Genetic Structures; Hereditary Disease; Indium; Industry; Libraries; Life; Link; Literature; Logic; Manuals; Mining; Molecular Biology; New England; Pharmacologic Substance; Phase; Polymerase; Polymerase Chain Reaction; Population; Process; Production; Property; PubMed; Publications; Reaction; Records; Research; Research Personnel; Resources; Role; Scientist; Seeds; Services; Sorting - Cell Movement; Source; Students; System; Testing; Work; Workplace; base; coping; cost; data integrity; data mining; design; design and construction; experience; graphical user interface; in vivo; information display; instructor; interest; member; novel; public health relevance; repository; text searching

DESCRIPTION (provided by applicant): This project concerns the design, construction and publication of a comprehensive database on DNA polymerases - DNAPolBase. DNAPolBase will serve as a freely available, high quality resource of DNA polymerase biochemistry, biology, genetics and structure. The worksite for this project will be New England Biolabs, in Ipswich, MA. The corruption of DNA copying is responsible for virtually all genetic disease states and the core machines responsible for this copying process are DNA polymerases. DNA polymerases are also key elements in a variety of diagnostic and molecular biology applications including DNA sequencing and polymerase chain reaction (PCR). Polymerase-based applications frequently involve non-natural substrates and reaction conditions, and engineered enzymes well suited to these applications are increasingly sought. The identification of novel polymerases and entire new super families has spurred renewed interest in these enzymes. This abundance of information makes it increasingly difficult for casual and experienced researchers alike to maintain a comprehensive view of the field. Despite their importance, no single repository for information relevant to the study and use of DNA polymerases currently exists. We will compile the wealth of known polymerase data from public and private records into a searchable database to provide a useful first step in understanding the role of these enzymes in vivo, and to inform their use in and adaptation to numerous applications. As part of the groundwork of database development, key collaborations with scientists willing to seed this database with their own libraries of private and public polymerase data and records have begun. From this auspicious start a database will be organized to serve the informational needs of numerous user groups, including basic and pharmaceutical researchers, application based scientists, instructors, and students. Functions from large-scale comparative studies (desired to advance many aspects of polymerase-based research and applications) to simple queries of polymerase properties or origins will be provided. We anticipate that NEB will benefit in much the same way that the public will by having access to such a resource, namely by advancing our internal DNA polymerase development projects. PUBLIC HEALTH RELEVANCE: DNA Polymerase Database Project Narrative DNA polymerases are the agents that copy the genetic blueprint of all living creatures, and corruption of their functions is the cause of virtually all genetic disease states. DNA polymerases have additionally been co-opted to serve as the engines of key diagnostic platforms, notably DNA-based detection systems and DNA sequencing. A central repository for information on DNA polymerases will be an important resource in understanding and coping with the effects of errant DNA copying, while additionally enhancing development of more rapid, effective, and cost-effective diagnostic platforms.

项目描述（由申请人提供）：本项目涉及DNA聚合酶综合数据库- DNAPolBase的设计、构建和发布。DNAPolBase将作为一个免费的、高质量的DNA聚合酶生物化学、生物学、遗传学和结构资源。这个项目的工作地点将是位于马萨诸塞州伊普斯维奇的新英格兰生物实验室。DNA复制的破坏是导致几乎所有遗传疾病状态的原因，而负责这一复制过程的核心机器是DNA聚合酶。DNA聚合酶也是多种诊断和分子生物学应用的关键元素，包括DNA测序和聚合酶链反应（PCR）。基于聚合酶的应用经常涉及非天然底物和反应条件，并且越来越多地寻求适合这些应用的工程酶。新的聚合酶和整个新的超家族的鉴定刺激了这些酶的新兴趣。如此丰富的信息使得普通和有经验的研究人员都越来越难以对该领域保持全面的看法。尽管它们很重要，但目前还没有一个与DNA聚合酶的研究和使用相关的信息库。我们将从公共和私人记录中收集丰富的已知聚合酶数据，并将其汇编成一个可搜索的数据库，为了解这些酶在体内的作用提供有用的第一步，并告知它们在许多应用中的使用和适应。作为数据库开发基础的一部分，与科学家的关键合作已经开始，这些科学家愿意将他们自己的私有和公共聚合酶数据和记录库作为这个数据库的种子。从这个良好的开端开始，将组织一个数据库，以满足众多用户群体的信息需求，包括基础和药物研究人员、基于应用的科学家、教师和学生。将提供从大规模比较研究（希望推进基于聚合酶的研究和应用的许多方面）到聚合酶性质或起源的简单查询的功能。我们预计NEB将以与公众相同的方式受益于获得这样的资源，即通过推进我们内部的DNA聚合酶开发项目。公共卫生相关性：DNA聚合酶数据库项目叙述DNA聚合酶是复制所有生物遗传蓝图的媒介，其功能的破坏是几乎所有遗传疾病状态的原因。DNA聚合酶也被用作关键诊断平台的引擎，特别是基于DNA的检测系统和DNA测序。DNA聚合酶信息的中心存储库将是理解和应对错误DNA复制影响的重要资源，同时还将促进更快速、有效和成本效益更高的诊断平台的开发。