DNA Polymerase Database

描述(由申请人提供)：该项目涉及DNA聚合酶-DNAPolBase综合数据库的设计、构建和出版。DNAPolBase将成为免费提供的DNA聚合酶生物化学、生物学、遗传学和结构的高质量资源。该项目的工作地点将是马萨诸塞州伊普斯维奇的新英格兰生物实验室。DNA复制的破坏是几乎所有遗传疾病状态的原因，而负责这一复制过程的核心机器是DNA聚合酶。DNA聚合酶也是各种诊断和分子生物学应用的关键元素，包括DNA测序和聚合酶链式反应(PCR)。基于聚合酶的应用经常涉及非天然底物和反应条件，并且越来越多地寻求适合于这些应用的工程酶。新的聚合酶和整个新的超家族的发现重新激发了人们对这些酶的兴趣。如此丰富的信息使非正式的和有经验的研究人员越来越难对该领域保持全面的看法。尽管它们很重要，但目前还不存在与DNA聚合酶的研究和使用相关的信息的单一储存库。我们将把来自公共和私人记录的丰富的已知聚合酶数据汇编成一个可搜索的数据库，以便为了解这些酶在体内的作用提供有用的第一步，并为它们在许多应用中的使用和适应提供信息。作为数据库开发基础工作的一部分，与愿意用自己的私人和公共聚合酶数据和记录图书馆播种该数据库的科学家的关键合作已经开始。从这一幸运的开始，数据库将被组织起来，以满足众多用户群体的信息需求，包括基础和药物研究人员、基于应用的科学家、教师和学生。将提供从大规模比较研究(希望推进基于聚合酶的研究和应用的许多方面)到简单的聚合酶性质或来源的查询的功能。我们预计，NEB将以与公众获得这种资源的方式大致相同的方式受益，即通过推进我们内部的DNA聚合酶开发项目。

DESCRIPTION (provided by applicant): This project concerns the design, construction and publication of a comprehensive database on DNA polymerases - DNAPolBase. DNAPolBase will serve as a freely available, high quality resource of DNA polymerase biochemistry, biology, genetics and structure. The worksite for this project will be New England Biolabs, in Ipswich, MA. The corruption of DNA copying is responsible for virtually all genetic disease states and the core machines responsible for this copying process are DNA polymerases. DNA polymerases are also key elements in a variety of diagnostic and molecular biology applications including DNA sequencing and polymerase chain reaction (PCR). Polymerase-based applications frequently involve non-natural substrates and reaction conditions, and engineered enzymes well suited to these applications are increasingly sought. The identification of novel polymerases and entire new super families has spurred renewed interest in these enzymes. This abundance of information makes it increasingly difficult for casual and experienced researchers alike to maintain a comprehensive view of the field. Despite their importance, no single repository for information relevant to the study and use of DNA polymerases currently exists. We will compile the wealth of known polymerase data from public and private records into a searchable database to provide a useful first step in understanding the role of these enzymes in vivo, and to inform their use in and adaptation to numerous applications. As part of the groundwork of database development, key collaborations with scientists willing to seed this database with their own libraries of private and public polymerase data and records have begun. From this auspicious start a database will be organized to serve the informational needs of numerous user groups, including basic and pharmaceutical researchers, application based scientists, instructors, and students. Functions from large-scale comparative studies (desired to advance many aspects of polymerase-based research and applications) to simple queries of polymerase properties or origins will be provided. We anticipate that NEB will benefit in much the same way that the public will by having access to such a resource, namely by advancing our internal DNA polymerase development projects.