GABAc Receptors & Mouse Retinal Ganglion Cell Responses
GABAc 受体
基本信息
- 批准号:7853857
- 负责人:
- 金额:$ 3.42万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2004
- 资助国家:美国
- 起止时间:2004-02-01 至 2010-01-31
- 项目状态:已结题
- 来源:
- 关键词:AgonistBiological ModelsBrainBrain regionButyric AcidsCellsCellular MorphologyComplementDataEnvironmentEvaluationEyeFeedbackFelis catusGlycineGoalsGoldfishIn VitroIndiumIndividualKnockout MiceKnowledgeLightLiteratureMediatingMorphologyMusNerve FibersOptic NerveOrganismOryctolagus cuniculusOutputPathway interactionsPhysiologicalPlayPreparationPrimatesPropertyRelative (related person)ReportingRetinaRetinalRetinal Ganglion CellsRoleSalamanderSamplingShapesSignal TransductionSpecificityStructureSystems AnalysisVisionVisualVisual Fieldscell typedefined contributionin vivointerestlight intensitymouse genomereceptorresponseretinal adaptationtheoriestoolvisual receptive field
项目摘要
DESCRIPTION (provided by applicant): Vision requires the integration of many aspects of the visual field into a cohesive whole that is used by the organism to navigate its environment. The mammalian retina is a laminar structure and each layer is populated by several cell types that combine to define the response properties of the retinal ganglion cells (RGCs), which transmit the signal to the brain. The many aspects of the visual scene are encoded by two parallel pathways within the retina that either detect increases or decreases in light intensity. There are many types of RGCs and their responses are defined by the inputs they receive. One key element in shaping their response properties is inhibition, which like other regions of the brain is mediated by glycine and gamma amino butyric acid (GABA). GABA inhibition is mediated by three receptors, GABAA, GABAB and GABAC, all of which are present in the retina. One challenge then is to determine how each of these inhibitory and excitatory inputs shapes the responses of the RGCs. A new tool towards attaining this goal is the ability to manipulate the mouse genome and selectively remove specific inputs, the effect of which then can be assessed on the RGCs. However, unlike the extensive literature for cat, rabbit and primate, relatively little is known about the fundamental properties of murine RGCs. The goal of this proposal is to characterize the response properties of mouse RGCs both in vivo and in vitro, and in particular determine the role that GABACR-mediated input plays in shaping the responses. The specific aims are (1) to determine the effect of eliminating, genetically, GABACR-mediated inhibition on a subset of RGC response properties recorded in vivo, and (2) correlate structure and function of a diverse set of RGCs, and determine the impact of eliminating GABACR-mediated input on the response properties of each of these cell types. These data will enhance our understanding of the role of inhibition in shaping the response of the output cells of the retina, and therefore important aspects of integration of information in the visual field.
描述(由申请人提供):视觉需要将视野的许多方面整合成一个有机体用来导航其环境的有凝聚力的整体。哺乳动物视网膜是层状结构,每层由几种细胞类型组成,这些细胞类型联合收割机组合以限定视网膜神经节细胞(RGC)的响应特性,视网膜神经节细胞将信号传递到大脑。视觉场景的许多方面是由视网膜内的两条平行通路编码的,这两条通路要么检测光强度的增加,要么检测光强度的减少。有许多类型的RGC,它们的响应由它们接收的输入定义。形成其反应特性的一个关键因素是抑制,这与大脑的其他区域一样,由甘氨酸和γ氨基丁酸(GABA)介导。GABA抑制由三种受体GABAA、GABAB和GABAC介导,所有这些受体都存在于视网膜中。然后,一个挑战是确定这些抑制性和兴奋性输入中的每一个如何塑造RGC的反应。实现这一目标的新工具是操纵小鼠基因组并选择性地去除特定输入的能力,然后可以评估其对RGC的影响。然而,与猫,兔和灵长类动物的大量文献不同,对小鼠RGC的基本性质知之甚少。该提案的目标是表征小鼠RGC在体内和体外的响应特性,特别是确定GABACR介导的输入在塑造响应中发挥的作用。具体目的是(1)确定消除遗传上GABACR介导的抑制对体内记录的RGC应答特性子集的影响,和(2)关联不同RGC组的结构和功能,并确定消除GABACR介导的输入对这些细胞类型中每种细胞类型的应答特性的影响。这些数据将增强我们对抑制在形成视网膜输出细胞的反应中的作用的理解,从而增强我们对视野中信息整合的重要方面的理解。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Maureen A McCall其他文献
Maureen A McCall的其他文献
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{{ truncateString('Maureen A McCall', 18)}}的其他基金
FASEB SRC Retinal Neurobiology & Visual Processing
FASEB SRC 视网膜神经生物学
- 批准号:
8675254 - 财政年份:2012
- 资助金额:
$ 3.42万 - 项目类别:
FASEB SRC Retinal Neurobiology & Visual Processing
FASEB SRC 视网膜神经生物学
- 批准号:
9068312 - 财政年份:2012
- 资助金额:
$ 3.42万 - 项目类别:
FASEB SRC Retinal Neurobiology & Visual Processing
FASEB SRC 视网膜神经生物学
- 批准号:
8459391 - 财政年份:2012
- 资助金额:
$ 3.42万 - 项目类别:
FASEB SRC Retinal Neurobiology & Visual Processing
FASEB SRC 视网膜神经生物学
- 批准号:
8316891 - 财政年份:2012
- 资助金额:
$ 3.42万 - 项目类别:
FASEB Summer Research Conference on Retinal Neurobiology and Visual Processing
FASEB 视网膜神经生物学和视觉处理夏季研究会议
- 批准号:
8004284 - 财政年份:2010
- 资助金额:
$ 3.42万 - 项目类别:
GABAc Receptors & Mouse Retinal Ganglion Cell Responses
GABAc 受体
- 批准号:
7502391 - 财政年份:2004
- 资助金额:
$ 3.42万 - 项目类别:
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