Metabolomics-Measured Urinary Metabolites, Diet & BP, 17 Population Samples: INTE

代谢组学测量的尿液代谢物、饮食

• 批准号: 7756585
• 负责人: JEREMIAH STAMLER
• 金额: $ 51.94万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-02-01 至 2011-08-17
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7756585
• 关键词: Abbreviations; Accounting; Acetates; Address; Administrator; Adult; Age; Albuminuria; Alcohol consumption; Alcohols; Algorithms; Amino Acids; Animals; Applications Grants; Arachidonic Acids; Area; Arts; Ascorbic Acid; Atherosclerosis; Bacteria; Basic Science; Behavior; Behavioral; Beverages; Bile Acids; Biochemical; Bioinformatics; Biological; Biological Markers; Blinded; Blood Pressure; Body mass index; Budgets; Carbohydrates; Carboxylic Acids; Chicago; Chinese People; Cholesterol; Collection; Commit; Complex; Country; Creatinine; Critiques; DM-Et; Data; Demographic Factors; Diabetes Mellitus; Diagnosis; Diet; Dietary Factors; Dietary Fats; Dietary Proteins; Diffusion; Dimensions; Discipline; Disease; Drug usage; Education; England; Epidemiologic Studies; Epidemiology; Equipment; Ethnic Origin; Evaluation; Event; Exclusion; Fiber; Folate; Food; Future; Genetic; Glossary; Goals; Hand; Hour; Hypertension; Hypotension; Immunoassay; Impaired Renal Function; Impairment; Individual; Intake; International; Japan; Joints; Journals; Kidney; Kidney Diseases; Knowledge; Laboratories; Lead; Left; Life; Life Style; Linoleic Acids; Linolenic Acids; London; Low Prevalence; Mass Spectrum Analysis; Measurement; Measures; Meat; Mentors; Metabolic; Metabolic Pathway; Methods; Microalbuminuria; Milk; Mission; Modeling; Molds; Monograph; National Heart, Lung, and Blood Institute; Nuclear Magnetic Resonance; Nutrient; Nutritional; Oligopeptides; Oryctolagus cuniculus; Paper; Participant; Pathway interactions; Pattern; Pattern Recognition; Peer Review; Penicillins; Peptide Fragments; Persons; Pharmaceutical Preparations; Phase; Population; Population Heterogeneity; Prevalence; Prevention; Prevention strategy; Principal Investigator; Proteins; Publications; Published Comment; Publishing; Quality Control; Questionnaires; Regression Analysis; Renal function; Reporting; Research; Research Personnel; Riboflavin; Risk; Risk Behaviors; Role; Sampling; Secure; Side; Signal Transduction; Societies; Soy Proteins; Specimen; Staging; Starch; Statistical Methods; Structure; Subgroup; Sum; Surveys; Syndrome; TOCSY; Taiwan; Techniques; Text; Thiamine; Time; Urine; Validation; Vegetable Proteins; Vegetables; Woman; Work; base; blood pressure regulation; cationic antimicrobial protein CAP 37; cohort; computerized; cost; cost effective; diabetic; dietary supplements; editorial; egg; experience; feeding; hypercholesterolemia; improved; macroalbuminuria; men; metabolomics; novel; organic acid; population based; premature; prevent; professor; programs; response; tool; trait; urinary

DESCRIPTION (provided by applicant): BACKGROUND: During 1997-99, the INTERMAP Study - with NHLBI support - surveyed 4,680 men and women ages 40-59 from 17 diverse population samples in Japan (4 samples), the Peoples Republic of China (PRC) (3 samples), the UK (2 samples), and the USA (8 samples). With standardized, quality-controlled methods, 4 24-hour dietary recalls, 2 timed 24-hour urine collections, 8 blood pressure (BP) measurements, anthropometric, and questionnaire data (on sociodemographic and biomedical traits) were accrued for all participants. Foods, beverages, and supplements were converted to nutrients (83 total) with use of up-dated, enhanced, standardized tables on nutrient composition (4 tables, 1/country). Urines were analyzed for Na, K, Ca, Mg, creatinine, albuminuria, amino acids. A high-quality central laboratory in London, England completed nuclear magnetic resonance (NMR) spectroscopic analyses of all urine specimens (2 from each of the 4, 680 participants plus 10% randomly selected, blinded split specimens for external quality control). All data underwent extensive quality control checks and were computerized and secured at the two INTERMAP International Coordinating Centers in Chicago and London, England. AIMS: Overall aim is - in a highly cost effective way - to add a new dimension of research information and thereby achieve a qualitative advance in knowledge on the etiopathogenesis of population-wide adverse BP levels (prehypertensive and hypertensive), i.e., develop a new epidemiologic area based on the urinary metabolic products whereby multiple dietary and diet-related factors influence BP. Based on the vast, unique array of high quality BP, nutritional, urinary metabolite, etc. data available and accruable, the specific aims are to identify and quantify - from NMR spectra in hand plus mass spectrometry and other techniques - urinary metabolites discriminatively related to BP and to each of several dietary (vegetable protein, soy protein, arachidonic acid, Na/K), diet-related sociodemographic (education, ethnicity) and anthropometric (body mass index) factors impacting BP. Advanced bioinformatics techniques and biochemical methods are to be used to address each aim. SIGNIFICANCE: The BP problem is population-wide; waiting for individuals to develop hypertension and then treating them with drugs is an inadequate strategy. This study - by identifying specific urinary metabolites implicated in the etiologic pathways whereby adverse dietary/lifestyle patterns lead to population-wide adverse BP levels - can help create fully effective strategies for prevention/eradication of the mass BP problem.

描述（由申请人提供）： 背景：1997-99 年期间，在 NHLBI 支持下的 INTERMAP 研究对来自日本（4 个样本）、中华民国（中国）（3 个样本）、英国（2 个样本）和美国（8 个样本）的 17 个不同人口样本的 4,680 名 40-59 岁的男性和女性进行了调查。通过标准化、质量控制的方法，为所有参与者积累了 4 次 24 小时饮食回忆、2 次定时 24 小时尿液收集、8 次血压 (BP) 测量、人体测量和问卷数据（关于社会人口统计学和生物医学特征）。使用更新的、增强的、标准化的营养成分表（4 个表，每个国家 1 个）将食品、饮料和补充剂转化为营养素（总共 83 种）。分析尿液中的钠、钾、钙、镁、肌酐、蛋白尿、氨基酸。英国伦敦的一个高质量中心实验室完成了所有尿液样本的核磁共振 (NMR) 光谱分析（4 名 680 名参与者各 2 份，加上 10% 随机选择的盲法分割样本，用于外部质量控制）。所有数据都经过了广泛的质量控制检查，并在芝加哥和英国伦敦的两个 INTERMAP 国际协调中心进行了计算机化和安全保护。目的：总体目标是以高度成本有效的方式增加研究信息的新维度，从而在全人群不良血压水平（高血压前期和高血压）的发病机制知识方面取得质的进步，即根据多种饮食和饮食相关因素影响血压的泌尿代谢产物开发一个新的流行病学领域。基于大量、独特的高质量血压、营养、尿代谢物等可用且可计算的数据，具体目标是通过手中的核磁共振波谱加上质谱和其他技术来识别和量化与血压和几种饮食（植物蛋白、大豆蛋白、花生四烯酸、Na/K）、饮食相关的社会人口统计学中的每一种有区别地相关的尿代谢物。 （教育、种族）和人体测量（体重指数）因素影响血压。将使用先进的生物信息学技术和生化方法来实现每个目标。意义：血压问题是整个人群的问题；等待个体出现高血压然后用药物治疗是不适当的策略。这项研究通过识别与不良饮食/生活方式模式导致人群不良血压水平的病因途径有关的特定尿液代谢物，可以帮助制定完全有效的策略来预防/根除大量血压问题。