INIA: Mouse Resources Core

INIA：鼠标资源核心

• 批准号: 7761305
• 负责人: Daniel Goldowitz
• 金额: $ 24.28万
• 依托单位: UNIVERSITY OF BRITISH COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-01 至 2012-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7761305
• 关键词: Acute; Alcohol consumption; Alcohols; Anxiety; Behavior; Behavioral; Boutos; Chronic; Communities; Complement; Convulsions; Corticosterone; Cryopreservation; Data; Data Base Management; Databases; Elements; Embryo; Equipment and supply inventories; Exhibits; Generations; Genetic; Genotype; Individual; Informatics; Information Dissemination; Knock-out; Knowledge; Maintenance; Measures; Microsatellite Repeats; Modeling; Molecular; Mus; Mutagenesis; Mutant Strains Mice; Neurosecretory Systems; Phenotype; Population; Production; Quality Control; Recombinants; Research Personnel; Resources; Screening procedure; Self Administration; Services; Single Nucleotide Polymorphism; Stress; Testing; Withdrawal; alcohol research; consomic; dependence relapse; experience; high throughput screening; mouse model; mutant; novel; operation; programs

DESCRIPTION (provided by applicant): This is a new core to INIA stress that arises out of the generation of many new mouse models through INIA support over the last 4+ years. These mouse models will be important resources to INIA investigators to study the interplay between stress, anxiety and alcohol consumption. With these new resources, we feel it is critical to disseminate them to users and evaluate each of these new mouse models with a set of INIA relevant behavioral analyses to better identify their usefulness to the various researchers in INIA stress and to the alcohol research community and beyond. The final part of this Core will provide key elements to a mouse core: distribution, cryopreservation, genotyping, and a curatorial function to keep an inventory of mouse mutants and inbred lines that we have available to INIA users and to accumulate and annotate the behavioral, cellular, and molecular knowledge about each mutant and inbred line. The specific aims of this Core are as follows: Aim 1 - The maintenance and distribution of novel mutant lines of mice produced by the ENU-mutagenesis program. The production and availability of novel mouse mutants that have abnormal alcohol, stress, and/or anxiety phenotypes represent an exciting resource for multi-disciplinary studies by our INIA stress investigators. This part of the Core will make these lines of mice available to INIA and community-wide researchers. Aim 2 - The maintenance and distribution of the expanded BXD recombinant inbred (Rl) and B6.A consomic lines of mice. This INIA has greatly expanded the phenotypic "space" (molecular, cellular, and behavioral) associated with unique mouse reference populations and this aspect of the Core will make these mice readily available to INIA researchers. This includes the 50 newly developed BXD Rl lines that arose, in part, from the previous INIA support, and the importation of 86.A consomic lines. Aim 3 - The Behavioral Phenotyping component of this Core will provide more comprehensive phenotypic information about EtOH and stress related behaviors in unique genetic mouse models that have been identified by high throughput behavioral screening within the INIA stress Consortium as exhibiting "extreme" phenotypes for EtOH and/or stress/anxiety responsiveness. Aim 4 - Affiliated functions that include curation of mouse lines in the Core, cryopreservation, database management, genotyping, and dissemination of information.

描述（由申请人提供）：这是INIA压力的一个新核心，是在过去4年多的INIA支持下产生的许多新小鼠模型。这些小鼠模型将成为INIA研究人员研究压力，焦虑和饮酒之间相互作用的重要资源。有了这些新的资源，我们认为至关重要的是将它们传播给用户，并通过一组INIA相关行为分析来评估这些新的小鼠模型，以更好地确定它们对INIA压力和酒精研究社区及其他领域的各种研究人员的有用性。本核心的最后一部分将提供小鼠核心的关键要素：分布，冷冻保存，基因分型和策展功能，以保持我们可供INIA用户使用的小鼠突变体和近交系的库存，并积累和注释有关每个突变体和近交系的行为，细胞和分子知识。该核心的具体目标如下：目标1 -通过ENU诱变程序产生的小鼠新突变系的维持和分布。具有异常酒精，压力和/或焦虑表型的新型小鼠突变体的生产和可用性代表了我们INIA压力研究人员进行多学科研究的令人兴奋的资源。核心的这一部分将使这些小鼠品系可供INIA和社区范围内的研究人员使用。目的2 -BXD重组小鼠近交系（R1）和B6.A重组小鼠近交系（B6.A）的维持和分布。该INIA极大地扩展了与独特小鼠参考群体相关的表型“空间”（分子、细胞和行为），核心的这一方面将使这些小鼠易于为INIA研究人员所用。这包括50个新开发的BXD R1系，其部分地由先前的INIA支持产生，以及86.A同源系的进口。目标3 -本核心的行为表型分析部分将提供关于独特遗传小鼠模型中EtOH和应激相关行为的更全面的表型信息，这些小鼠模型已通过INIA应激联盟内的高通量行为筛选鉴定为表现出EtOH和/或应激/焦虑反应的“极端”表型。目标4 -附属功能，包括核心小鼠品系的管理、冷冻保存、数据库管理、基因分型和信息传播。