INIA: Mouse Resources Core

INIA：鼠标资源核心

• 批准号: 7761305
• 负责人: Daniel Goldowitz
• 金额: $ 24.28万
• 依托单位: UNIVERSITY OF BRITISH COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-01 至 2012-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7761305
• 关键词: Acute; Alcohol consumption; Alcohols; Anxiety; Behavior; Behavioral; Boutos; Chronic; Communities; Complement; Convulsions; Corticosterone; Cryopreservation; Data; Data Base Management; Databases; Elements; Embryo; Equipment and supply inventories; Exhibits; Generations; Genetic; Genotype; Individual; Informatics; Information Dissemination; Knock-out; Knowledge; Maintenance; Measures; Microsatellite Repeats; Modeling; Molecular; Mus; Mutagenesis; Mutant Strains Mice; Neurosecretory Systems; Phenotype; Population; Production; Quality Control; Recombinants; Research Personnel; Resources; Screening procedure; Self Administration; Services; Single Nucleotide Polymorphism; Stress; Testing; Withdrawal; alcohol research; consomic; dependence relapse; experience; high throughput screening; mouse model; mutant; novel; operation; programs

DESCRIPTION (provided by applicant): This is a new core to INIA stress that arises out of the generation of many new mouse models through INIA support over the last 4+ years. These mouse models will be important resources to INIA investigators to study the interplay between stress, anxiety and alcohol consumption. With these new resources, we feel it is critical to disseminate them to users and evaluate each of these new mouse models with a set of INIA relevant behavioral analyses to better identify their usefulness to the various researchers in INIA stress and to the alcohol research community and beyond. The final part of this Core will provide key elements to a mouse core: distribution, cryopreservation, genotyping, and a curatorial function to keep an inventory of mouse mutants and inbred lines that we have available to INIA users and to accumulate and annotate the behavioral, cellular, and molecular knowledge about each mutant and inbred line. The specific aims of this Core are as follows: Aim 1 - The maintenance and distribution of novel mutant lines of mice produced by the ENU-mutagenesis program. The production and availability of novel mouse mutants that have abnormal alcohol, stress, and/or anxiety phenotypes represent an exciting resource for multi-disciplinary studies by our INIA stress investigators. This part of the Core will make these lines of mice available to INIA and community-wide researchers. Aim 2 - The maintenance and distribution of the expanded BXD recombinant inbred (Rl) and B6.A consomic lines of mice. This INIA has greatly expanded the phenotypic "space" (molecular, cellular, and behavioral) associated with unique mouse reference populations and this aspect of the Core will make these mice readily available to INIA researchers. This includes the 50 newly developed BXD Rl lines that arose, in part, from the previous INIA support, and the importation of 86.A consomic lines. Aim 3 - The Behavioral Phenotyping component of this Core will provide more comprehensive phenotypic information about EtOH and stress related behaviors in unique genetic mouse models that have been identified by high throughput behavioral screening within the INIA stress Consortium as exhibiting "extreme" phenotypes for EtOH and/or stress/anxiety responsiveness. Aim 4 - Affiliated functions that include curation of mouse lines in the Core, cryopreservation, database management, genotyping, and dissemination of information.

描述(由申请人提供)：这是INIA压力的新核心，在过去4年多的时间里，通过INIA支持生成了许多新的鼠标模型。这些小鼠模型将成为INIA研究人员研究压力、焦虑和饮酒之间相互作用的重要资源。有了这些新的资源，我们认为至关重要的是将它们传播给用户，并使用一组INIA相关行为分析来评估这些新的小鼠模型中的每一个，以更好地确定它们对INIA压力中的各种研究人员以及酒精研究社区和其他方面的用处。这个核心的最后部分将提供小鼠核心的关键元素：分布、冷冻保存、基因分型和管理功能，以保存我们可供INIA用户使用的小鼠突变和近交系的清单，并积累和注释关于每个突变和近交系的行为、细胞和分子知识。该核心的具体目标如下：目标1-由ENU-突变计划产生的小鼠新突变系的维护和分布。具有异常酒精、压力和/或焦虑表型的新型小鼠突变体的产生和可获得性为我们的INIA应激研究人员提供了一个令人兴奋的多学科研究资源。核心的这一部分将为INIA和全社区的研究人员提供这些品系的小鼠。目的2-扩大的BXD重组近交系(RL)和B6小鼠A染色体系的维持和分布。这种INIA极大地扩展了与独特的小鼠参考种群相关的表型“空间”(分子、细胞和行为)，核心的这一方面将使这些小鼠易于为INIA研究人员所用。这包括新育成的50个BXD RL品系，部分来自以前的INIA支持，以及86.A共体品系的引进。目的3-本核心的行为表型组件将提供关于乙醇和压力相关行为的更全面的表型信息，这些独特的遗传小鼠模型已被INIA压力联盟内的高通量行为筛查确定为表现出乙醇和/或压力/焦虑反应的“极端”表型。AIM 4-附属职能，包括核心小鼠品系的管理、冷冻保存、数据库管理、基因分型和信息传播。