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Tissue engineering of human myometrium

人体子宫肌层组织工程

基本信息

批准号：
7901617
负责人：
ROGER C. YOUNG
金额：
$ 18.62万
依托单位：
UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-08-01 至 2012-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7901617
关键词：
3-Dimensional Address Adherent Culture Biochemical Markers Catheters Cell Count Cell Survival Cell physiology Cells Characteristics Chemicals Chitin Chitosan Contracts Crustacea Culture Techniques Cultured Cells Defect Diffusion Dimensions Engineering Functional disorder Gel Gene Expression Generations Health Hemorrhage Human Hysterectomy Isometric Contraction Knowledge Lateral Measures Modeling Modification Molds Molecular Monitor Muscle Cells Myometrial Normal tissue morphology Nutrient Onions Organ Parents Pathway interactions Patients Penetration Phase Phenotype Physiology Porosity Pregnancy Premature Labor Publishing Sampling Seeds Shapes Skin Smooth Muscle Solid Structure Study models Supporting Cell Techniques Temperature Thick Time Tissue Engineering Tissues Uterus Woman Work cell growth density exoskeleton interest migration myometrium novel pregnant pressure public health relevance repaired response scaffold scale up three-dimensional modeling uterine smooth muscle cell

项目摘要

DESCRIPTION (provided by applicant): We intend to grow human uterine tissue (myometrium) in three dimensions. We will use a novel scaffold-forming technique, and then seed cells into the scaffold. These structures will be made large enough to mimic normal human myometrium. This project will also demonstrate that this engineered tissue will function similarly to the normal tissue. We will show that the engineered tissue strips are capable of contracting, and the engineered uterus is capable of generating intracavitary pressure. We will develop tissue engineering as a new model for the study of contractions of the pregnant human uterus. This model will address major health questions of abnormal uterine function (such as preterm labor, post partum hemorrhage, and post-dates pregnancy). The novelty of the model is that both cellular gene expression and 3-D physical structure are capable of being controlled. We will start with human uterine tissue obtained from women at the time of Cesarean Delivery. We will amplify the number of cells by growing them using standard culture techniques. We will create scaffolds from chitosan, a readily available, naturally occurring, safe, bio-resorbable compound. These scaffolds can be made in virtually any shape and with a wide variety of porosities to support cell growth. We will determine the rates that cells grow into and around the scaffolds. We will compare the biochemical markers found in normal uterine tissue with those in the engineered tissue. We will compare the physiology of engineered tissue strips with strips recently removed from women. We will demonstrate, for the first time, generation of intracavitary pressure from a tissue-engineered uterus. In addition to creating a new paradigm for study of tissue-level and organ-level uterine function, these techniques are capable of being up-scaled to allow tissue patches to be created from a small smooth muscle sample from a patient. This project is the first step on the path to creating an entire replacement uterus for women born without a functional uterus, or who have had a hysterectomy. PUBLIC HEALTH RELEVANCE: Tissue-engineered myometrium will be made from human uterine cells and chitosan, a naturally occurring, resorbable scaffold material. The tissue-engineered myometrium will be a novel model for the study of myometrial contractility. This project will be the first step toward construction of a functional replacement uterus for women who were born with a uterine anomaly or have had a hysterectomy.

描述（由申请人提供）：我们打算在三维空间中培养人类子宫组织（子宫肌层）。我们将使用一种新的支架形成技术，然后将细胞植入支架。这些结构将被做得足够大，以模仿正常的人类肌层。该项目还将证明这种工程组织的功能与正常组织相似。我们将展示工程组织条能够收缩，工程子宫能够产生腔内压力。我们将发展组织工程作为研究妊娠人子宫收缩的新模式。该模型将解决子宫功能异常的主要健康问题（如早产、产后出血和过期怀孕）。该模型的新颖之处在于细胞基因表达和三维物理结构都能够被控制。我们将从剖宫产时从妇女身上获得的人类子宫组织开始。我们将使用标准的培养技术来扩增细胞的数量。我们将用壳聚糖制作支架，壳聚糖是一种现成的、天然存在的、安全的、生物可吸收的化合物。这些支架几乎可以制成任何形状，并具有各种孔隙度来支持细胞生长。我们将测定细胞在支架内和支架周围生长的速率。我们将比较在正常子宫组织和工程组织中发现的生化标记物。我们将比较工程组织条和最近从女性身上取下的组织条的生理机能。我们将首次演示，从组织工程子宫产生腔内压力。除了为研究组织水平和器官水平的子宫功能创造了一个新的范例外，这些技术还能够扩大规模，允许从患者的小块平滑肌样本中创建组织补丁。这个项目是为天生没有功能子宫或子宫切除术的妇女创造完整替代子宫的第一步。公共卫生相关性：组织工程肌层将由人类子宫细胞和壳聚糖制成，壳聚糖是一种天然存在的可吸收支架材料。组织工程化的子宫肌将成为研究子宫肌收缩性的新模型。该项目将是为子宫畸形或子宫切除的女性构建功能性替代子宫的第一步。