ELECTROPHYSIOLOGY OF HUMAN REPRODUCTIVE SMOOTH MUSCLE
ELECTROPHYSIOLOGY OF HUMAN REPRODUCTIVE SMOOTH MUSCLE
批准号:
3081355
负责人:
ROGER C. YOUNG
金额:
$5.57万
依托单位国家:
美国
项目类别:
财政年份:
1988
资助国家:
美国
项目状态:
已结题
起止时间:
1988-04-25 至 1993-03-31
关键词:
action potentials aminophylline birth electrical conductance electrophysiology epinephrine human subject ion transport lidocaine magnesium membrane potentials microelectrodes muscle cells myometrium nifedipine norepinephrine oxytocin pregnancy reproductive system pharmacology smooth muscle sulfates terbutaline tissue /cell culture voltage /patch clamp
中文摘要
早产和分娩是一个深刻的全国性问题
重要性。作为临床医生,我们在预防和治疗方面受到阻碍
对早产的认识不足对早产的治疗
开始分娩、维持分娩的微观细节
劳工,以及可能的干预点。
为了定义人类电生理学的细节
生殖平滑肌和药物作用机制,
我们建议在此进行分离的微电极研究
妊娠人子宫肌细胞及其培养。
一种用于酶促分散和分离个体的方案
将对妊娠人子宫肌层的肌细胞进行检测。
在孕妇血清存在的情况下培养的细胞也将
被研究以确定怀孕激素的影响
电生理参数。
在过去的十年里,膜片钳技术已经
开发和利用以研究多种类型的细胞,包括
神经学、心肌和平滑肌。膜片钳是一种
允许各种类型的测量的专门技术
将会被执行。使用“全电池”电压钳位,
特定离子对细胞兴奋性的贡献将是
下定决心。
膜片钳技术的一个强大方面是能够
研究小片状的膜。这项技术将使
离子特异性通道的观察及药物对其影响
频道。
英文摘要
Preterm labor and deliver is a problem of profound national
importance. As clinicians, we are hindered in the prevention and
treatment of preterm labor by a lack of understanding of the
microscopic details of the initiation of labor, maintenance of
labor, and possible points of intervention.
In order to define the details of the electrophysiology of human
reproductive smooth muscle and the mechanisms of drug action,
we propose here to perform microelectrode studies on isolated
pregnant human uterine myocytes and their cultures.
A protocol for the enzymatic dispersion and isolation of individual
myocytes from pregnant human myometrium will be determined.
Cells cultured in the presence of pregnant human serum will also
be studied to determine the effects of the hormones of pregnancy
on electrophysiologic parameters.
Over the past ten years the technique of patch clamping has been
developed and exploited to study many types of cells, including
neurologic, cardiac, and smooth muscle. The patch clamp is a
specialized technique that allows various types of measurements
to be performed. Using the "whole cell" voltage clamp, the
contributions of specific ions to the excitability of the cell will be
determined.
A powerful aspect of the patch clamp technique is in the ability
to study small patches of membrane. This technique will allow
observation of ion specific channels, and the effects of drugs on
the channels.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Labor Status Monitor for diagnosing True versus False Labor in preterm patients
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批准号:10484554
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项目类别:
-
资助金额:$137.55万
-
财政年份:2022
-
负责人:ROGER C. YOUNG
-
依托单位:
Identifying the precursor to oxytocin-induced uterine tachysystole by monitoring with uterine EMG
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批准号:9752933
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项目类别:
-
资助金额:$20.3万
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财政年份:2019
-
负责人:ROGER C. YOUNG
-
依托单位:
Tissue engineering of human myometrium
-
批准号:7901617
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项目类别:
-
资助金额:$18.62万
-
财政年份:2009
-
负责人:ROGER C. YOUNG
-
依托单位:
Tissue engineering of human myometrium
-
批准号:7701345
-
项目类别:
-
资助金额:$18.81万
-
财政年份:2009
-
负责人:ROGER C. YOUNG
-
依托单位:
FACTORS RESPONSIBLE FOR THE CONTROL OF HUMAN LABOR
-
批准号:2758503
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项目类别:
-
资助金额:$21.97万
-
财政年份:1999
-
负责人:ROGER C. YOUNG
-
依托单位:
FACTORS RESPONSIBLE FOR THE CONTROL OF HUMAN LABOR
-
批准号:6138839
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项目类别:
-
资助金额:$20.0万
-
财政年份:1999
-
负责人:ROGER C. YOUNG
-
依托单位:
FACTORS RESPONSIBLE FOR THE CONTROL OF HUMAN LABOR
-
批准号:6627383
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项目类别:
-
资助金额:$11.79万
-
财政年份:1999
-
负责人:ROGER C. YOUNG
-
依托单位:
FACTORS RESPONSIBLE FOR THE CONTROL OF HUMAN LABOR
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批准号:6654451
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项目类别:
-
资助金额:$23.19万
-
财政年份:1999
-
负责人:ROGER C. YOUNG
-
依托单位:
FACTORS RESPONSIBLE FOR THE CONTROL OF HUMAN LABOR
-
批准号:6343215
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项目类别:
-
资助金额:$20.23万
-
财政年份:1999
-
负责人:ROGER C. YOUNG
-
依托单位:
FACTORS RESPONSIBLE FOR THE CONTROL OF HUMAN LABOR
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批准号:6490423
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项目类别:
-
资助金额:$9.77万
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财政年份:1999
-
负责人:ROGER C. YOUNG
-
依托单位:
ELECTROPHYSIOLOGY OF HUMAN REPRODUCTIVE SMOOTH MUSCLE
-
批准号:3081354
-
项目类别:
-
资助金额:$5.83万
-
财政年份:1988
-
负责人:ROGER C. YOUNG
-
依托单位:
ELECTROPHYSIOLOGY OF HUMAN REPRODUCTIVE SMOOTH MUSCLE
-
批准号:3081353
-
项目类别:
-
资助金额:$2.58万
-
财政年份:1988
-
负责人:ROGER C. YOUNG
-
依托单位:
ELECTROPHYSIOLOGY OF HUMAN REPRODUCTIVE SMOOTH MUSCLE
-
批准号:3081358
-
项目类别:
-
资助金额:$3.71万
-
财政年份:1988
-
负责人:ROGER C. YOUNG
-
依托单位:
ELECTROPHYSIOLOGY OF HUMAN REPRODUCTIVE SMOOTH MUSCLE
-
批准号:3081357
-
项目类别:
-
资助金额:$7.2万
-
财政年份:1988
-
负责人:ROGER C. YOUNG
-
依托单位:
ELECTROPHYSIOLOGY OF HUMAN REPRODUCTIVE SMOOTH MUSCLE
-
批准号:3081356
-
项目类别:
-
资助金额:$5.62万
-
财政年份:1988
-
负责人:ROGER C. YOUNG
-
依托单位:
海外基金