Monkey Cortical Connections Database

描述(由申请人提供)：灵长类动物大脑皮质连接的知识对于了解感觉、感知和认知的高级大脑机制及其障碍是必不可少的，但联系追踪研究越来越罕见，较旧的研究只能通过代表早期研究人员对数据的解释的出版物获得，并且通常无法获得原始数据以进行重新解释。检索实验材料档案和将其转换为可访问的数字形式，在后勤和其他方面都存在相当大的障碍。我们寻求开发一个全面且持续的材料档案，展示恒河猴大脑中皮质和皮质下的连接，其中来自个别实验的所有组织学材料都以数字形式提供，并有一个合适的搜索引擎向所有研究人员开放访问。通过这种方式，原始实验数据可以在积累的功能和临床数据的基础上，随着皮质和皮质下结构的新部分的产生而被注释和重新解释。目前的建议建立在构建灵长类大脑结构的高分辨率显微地图集方面的专业知识，并将创建一个可搜索的成年恒河猴大脑皮质连接的数据库。将建立一条管道，向一系列猴子的目标皮质区域注射逆行和顺行运输的示踪剂。有关每个单独大脑的数字数据将通过以下方式收集：结构磁共振；在大脑切片过程中移除每个40&lt；m的切片时对切片的块面进行连续成像；通过大脑的交替切片的高分辨率成像，以运输的示踪剂进行染色或尼塞尔染色；将切片的图像扭曲回到大脑的块面图像和磁共振成像，以提供每个大脑的3D体积数据集。将在其中存储单个数据集的可搜索数据库将包括与猕猴大脑的标准高分辨率数字地图集相关的注释来自每个实验大脑的数据集的能力、按名称或通过肉眼检查注射区域或可能的传入来源和传出目标来查询连接的能力、与其他相关神经科学信息的数据库互操作的能力。该数据库还将允许纳入从出版物和(或)现有幻灯片材料中提取的连接性数据集。结果将是一个活生生的档案，对理解神经和神经精神障碍的病理生理学至关重要的数据可以继续添加和查询。关于灵长类大脑皮层功能区如何相互连接的公共卫生相关知识，对于理解感觉、知觉和认知的大脑机制及其障碍至关重要，特别是与脑功能成像有关的机制。然而，相关的实验数据变得越来越难获得，因为所做的研究很少，没有以较旧的出版物形式提供原始数据的访问，而且他们也没有此类信息的档案。我们寻求开发一个全面的、持续的材料档案，以微观分辨率展示恒河猴大脑皮质和皮质下的连通性。其中，来自个别实验的所有材料都以数字形式提供，并有一个合适的搜索引擎向所有研究人员开放访问，并与其他神经科学信息数据库相互关联。结果将是一个活生生的档案，对理解神经和神经精神障碍的病理生理学至关重要的数据可以继续添加和查询。

DESCRIPTION (provided by applicant): Knowledge of cerebral cortical connectivity in primates is essential for understanding higher brain mechanisms of sensation, perception and cognition and their disorders but connection tracing studies are increasingly uncommon, older studies are accessible only through publications which represent an earlier investigator's interpretation of the data, and access to the original data for re-interpretation is commonly unavailable. Retrieval of archives of experimental material and conversion to an accessible digital form presents considerable logistical and other handicaps. We seek to develop a comprehensive and ongoing archive of material demonstrating cortical and subcortical connectivity in rhesus monkey brains, in which all the histological material from individual experiments is available in its digital form, with a suitable search engine providing open access to all investigators. In this way, original experimental data can be annotated and re-interpreted as newer parcellations of cortical and subcortical structures are made on the basis of accumulating functional and clinical data. The current proposal builds on expertise in constructing high resolution microscopic atlases of primate brain architecture and will create a searchable database of connections of the cerebral cortex in the adult rhesus monkey. A pipeline will be established in which targeted cortical areas in a series of monkeys will be injected with a tracer transported both retrogradely and anterogradely. Digital data about each individual brain will be collected by means of: structural mri; serial imaging of the sectioned blockface as each 40<m section is removed during sectioning of the brain; imaging at high resolution of alternate sections through the brain stained for the transported tracer or stained with the nissl stain; warping images of the sections back onto the blockface images of the brain and mris to provide a 3d volumetric dataset for each brain. The searchable database into which the individual datasets will be stored will incorporate a capacity to annotate the dataset from each experimental brain in relation to a standard high resolution digital atlas of the rhesus monkey brain, a capacity for making queries about connections by name or by visual inspection of injected regions or of likely sources of afferents and targets of efferents, a capacity for interoperability with databases of other relevant neuroscience information. The database will also permit incorporation of datasets of connectivity drawn from material in publications and/or existing slide. The result will be a living archive to which data essential for the understanding of the pathophysiology of neurological and neuropsychiatric disorders can continue to be added and queried. PUBLIC HEALTH RELEVANCE Knowledge of how functional regions of the primate cerebral cortex are connected with one another is essential for understanding brain mechanisms of sensation, perception and cognition and their disorders, especially in relation to functional brain imaging. Relevant experimental data however is becoming hard to obtain because few studies are being done, access to original data is not provided in older forms of publication and theire are no archives of such information. We seek to develop a comprehensive and ongoing archive of material demonstrating cortical and subcortical connectivity in rhesus monkey brains at microscopic resolution. In it, all the material from individual experiments is available in digital form, with a suitable search engine providing open access to all investigators and interrelated with other databases of neuroscientific information. The result will be a living archive to which data essential for the understanding of the pathophysiology of neurological and neuropsychiatric disorders can continue to be added and queried.