Monkey Cortical Connections Database

描述（由申请人提供）：灵长类动物大脑皮质连接性的知识对于了解感觉、知觉和认知及其障碍的高级大脑机制至关重要，但连接追踪研究越来越少见，旧的研究只能通过代表早期研究者的出版物访问对数据的解释，并且通常无法访问用于重新解释的原始数据。 检索实验材料档案和转换成可查阅的数字形式存在着相当大的后勤和其他障碍。 我们寻求开发一个全面的和持续的材料档案，展示恒河猴大脑皮层和皮层下的连接，其中所有来自个体实验的组织学材料都以数字形式提供，并有一个合适的搜索引擎为所有研究人员提供开放访问。 通过这种方式，原始的实验数据可以被注释和重新解释为皮质和皮质下结构的更新的包裹，积累功能和临床数据的基础上。 目前的提议建立在构建灵长类动物大脑结构的高分辨率显微镜图谱的专业知识基础上，并将创建一个成年恒河猴大脑皮层连接的可搜索数据库。 将建立一个管道，其中将向一系列猴的靶向皮质区域注射逆行和顺行运输的示踪剂。 将通过以下方式收集关于每个个体脑的数字数据：结构MRI;在脑切片期间当每个40 μ m切片被移除时对切片的块面的连续成像;通过对运输示踪剂染色或用尼氏染色剂染色的脑的交替切片的高分辨率成像;将切片的图像扭曲回到大脑和MRI的块面图像上，以提供每个大脑的3D体积数据集。 将存储单个数据集的可搜索数据库将结合注释来自每个实验大脑的数据集的能力，其与恒河猴大脑的标准高分辨率数字图谱有关，通过名称或通过对注入区域或传入和传出目标的可能来源的视觉检查来查询连接的能力，与其他相关神经科学信息数据库的互操作能力。 该数据库还将允许纳入从出版物和/或现有幻灯片材料中提取的连通性数据集。 其结果将是一个活的档案，其中的数据，神经和神经精神疾病的病理生理学的理解至关重要，可以继续添加和查询。 灵长类动物大脑皮层的功能区域如何相互连接的知识对于理解感觉、知觉和认知及其障碍的大脑机制至关重要，特别是与功能性大脑成像有关的知识。 然而，相关的实验数据越来越难获得，因为很少有研究正在进行，原始数据的访问不提供旧形式的出版物，他们没有这些信息的档案。 我们寻求开发一个全面且持续的材料档案，以显微镜分辨率展示恒河猴大脑皮质和皮质下的连接性。 在它里面，所有来自个体实验的材料都以数字形式提供，有一个合适的搜索引擎为所有研究者提供开放访问，并与其他神经科学信息数据库相互关联。 其结果将是一个活的档案，其中的数据，神经和神经精神疾病的病理生理学的理解至关重要，可以继续添加和查询。

DESCRIPTION (provided by applicant): Knowledge of cerebral cortical connectivity in primates is essential for understanding higher brain mechanisms of sensation, perception and cognition and their disorders but connection tracing studies are increasingly uncommon, older studies are accessible only through publications which represent an earlier investigator's interpretation of the data, and access to the original data for re-interpretation is commonly unavailable. Retrieval of archives of experimental material and conversion to an accessible digital form presents considerable logistical and other handicaps. We seek to develop a comprehensive and ongoing archive of material demonstrating cortical and subcortical connectivity in rhesus monkey brains, in which all the histological material from individual experiments is available in its digital form, with a suitable search engine providing open access to all investigators. In this way, original experimental data can be annotated and re-interpreted as newer parcellations of cortical and subcortical structures are made on the basis of accumulating functional and clinical data. The current proposal builds on expertise in constructing high resolution microscopic atlases of primate brain architecture and will create a searchable database of connections of the cerebral cortex in the adult rhesus monkey. A pipeline will be established in which targeted cortical areas in a series of monkeys will be injected with a tracer transported both retrogradely and anterogradely. Digital data about each individual brain will be collected by means of: structural mri; serial imaging of the sectioned blockface as each 40<m section is removed during sectioning of the brain; imaging at high resolution of alternate sections through the brain stained for the transported tracer or stained with the nissl stain; warping images of the sections back onto the blockface images of the brain and mris to provide a 3d volumetric dataset for each brain. The searchable database into which the individual datasets will be stored will incorporate a capacity to annotate the dataset from each experimental brain in relation to a standard high resolution digital atlas of the rhesus monkey brain, a capacity for making queries about connections by name or by visual inspection of injected regions or of likely sources of afferents and targets of efferents, a capacity for interoperability with databases of other relevant neuroscience information. The database will also permit incorporation of datasets of connectivity drawn from material in publications and/or existing slide. The result will be a living archive to which data essential for the understanding of the pathophysiology of neurological and neuropsychiatric disorders can continue to be added and queried. PUBLIC HEALTH RELEVANCE Knowledge of how functional regions of the primate cerebral cortex are connected with one another is essential for understanding brain mechanisms of sensation, perception and cognition and their disorders, especially in relation to functional brain imaging. Relevant experimental data however is becoming hard to obtain because few studies are being done, access to original data is not provided in older forms of publication and theire are no archives of such information. We seek to develop a comprehensive and ongoing archive of material demonstrating cortical and subcortical connectivity in rhesus monkey brains at microscopic resolution. In it, all the material from individual experiments is available in digital form, with a suitable search engine providing open access to all investigators and interrelated with other databases of neuroscientific information. The result will be a living archive to which data essential for the understanding of the pathophysiology of neurological and neuropsychiatric disorders can continue to be added and queried.