Console upgrades for biological NMR spectrometers

描述（由申请人提供）：申请资金用于将位于范德比尔特大学结构生物学中心（VCSB）的配备低温探针的600 MHz和800 MHz核磁共振光谱仪的旧核磁共振控制台更换为最先进的控制台。目前用于VCSB溶液核磁共振光谱仪的Bruker DRX控制台已经有8年的历史了，技术上已经过时，并且开始频繁发生故障。资金要求将与机构承诺汇集在一起，用Bruker Avance III游戏机取代老化的2台最关键的游戏机。除了提高核磁共振设备的可靠性外，新的控制台及其集成软件预计将比当前过时的系统具有更高的性能，并且还具有扩展的技术能力。寻求新控制台的仪器由专门的核磁共振设施经理Markus Voehler博士每天监督，他是一位有超过20年生物分子系统工作经验的成功的核磁共振波谱学家。新控制台对于美国国立卫生研究院资助的生物分子研究的成功至关重要，这四个主要用户（C. Sanders， W. Chazin， M. P. Stone, a . Krezel）都是生物分子核磁共振方面的专家，而次要用户（在本应用程序中由B. Eichman， B. Hudson和J. Meiler代表）。用户组的工作包括从核磁共振角度要求极高的研究，包括对整体膜蛋白、大可溶性蛋白和总分子量在100 kD范围内的蛋白-蛋白复合物的溶液核磁共振研究。一些项目涉及与人类疾病直接相关的生物分子问题。与公共卫生相关：核磁共振（NMR）光谱学是一种强大的技术，用于研究生物分子的三维结构、运动和相互作用。范德比尔特大学的一些美国国立卫生研究院支持的研究人员使用核磁共振来研究与人类健康和疾病直接或间接相关的生物分子。该应用程序要求用最先进的设备替换过时和越来越不可靠的核磁共振设备所需的资金，这对于与该应用程序相关的研究人员的生物和生物医学研究计划的持续成功至关重要，并且越来越多的用户刚刚意识到核磁共振的力量。

DESCRIPTION (provided by applicant): Funds are requested for the replacement of old NMR consoles for cryoprobe-equipped 600 MHz and 800 MHz NMR spectrometers located in the Vanderbilt University Center for Structural Biology (VCSB) with state-of-the-art consoles. The current Bruker DRX consoles for the solution NMR spectrometers of the VCSB are now 8 years old and are technically out of date and beginning to break down on a frequent basis. Funds are requested that will be pooled with institutional commitments to replace the 2 most critical of the aging consoles with Bruker Avance III consoles. In addition to enhancing the reliability of the NMR equipment, the new consoles and their integrated software are expected to perform at a much higher level than the current out-of-date systems and also to have expanded technical capabilities. The instrumentation for which the new consoles are sought is overseen on a daily basis by a dedicated NMR facilities manager, Dr. Markus Voehler, an accomplished NMR spectroscopist with over 20 years of experience working on biomolecular systems. The new consoles are critical to the success of the NIH-funded biomolecular research of both a group of four major users (C. Sanders, W. Chazin, M. P. Stone, A. Krezel), all of whom are experts in biomolecular NMR, and of minor users (represented in this application by B. Eichman, B. Hudson, and J. Meiler). Included in the work of the user groups are studies that are extremely demanding from an NMR standpoint, including solution NMR studies of integral membrane proteins, large soluble proteins and protein-protein complexes that have aggregate molecular weights in the range of 100 kD. A number of the projects involve biomolecular problems of direct relevance to human disease. PUBLIC HEALTH RELEVANCE: Nuclear magnetic resonance (NMR) spectroscopy is a powerful technique that is used to study the three-dimensional structures, motions, and interactions of biological molecules. NMR is used by a number of NIH-supported investigators at Vanderbilt University to study biomolecules that are of both direct and indirect relevance to human health and disease. The application requests the funds required to replace out-of-date and increasingly-unreliable NMR equipment with fully state-of-the-art equipment, as is critical to the continuing success of the biological and biomedical research programs of the investigators associated with this application and a growing community of additional users who are just realizing the power of NMR.