Acquisition of a Hybrid LC/MS/MS to Enhance Research in Nevada

购买混合 LC/MS/MS 以加强内华达州的研究

• 批准号: 7594901
• 负责人: David Roger Quilici
• 金额: $ 47.25万
• 依托单位: UNIVERSITY OF NEVADA RENO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-15 至 2010-05-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7594901
• 关键词: Address; Area; Arts; Biochemistry; Biotechnology; Calcium Signaling; Cell Death; Chloride Channels; Core Facility; Detection; Education; Faculty; Funding; Funding Applicant; Heart Diseases; Hybrids; Institutes; Institution; Liquid Chromatography; Malignant Neoplasms; Mass Spectrum Analysis; Muscular Dystrophies; N.I.H. Research Support; Neurons; Nevada; Physiology; Plants; Post-Translational Protein Processing; Protein-Protein Interaction Map; Proteins; Proteomics; Regulation; Reproducibility; Research; Research Training; Resolution; Sampling; Services; Site; Smooth Muscle; Stem cells; Stress; System; Technology; Training; Ulcerative Colitis; Universities; anticancer research; base; biological research; diabetic gastroparesis; graduate student; instrument; instrumentation; liquid chromatography mass spectrometry; mass spectrometer; member; muscular structure; nano; neoplastic cell; neuronal growth; programs; public health relevance; senescence; success; training project

DESCRIPTION (provided by applicant): Funds are requested to purchase a QSTAR Elite Hybrid LC-MS/MS mass spectrometer and Tempo nano multi-dimensional liquid chromatography (MDLC) system for the Nevada Proteomics Center (NPC). The instrument being requested is an electrospray- interfaced instrument for on-line liquid chromatography-mass spectrometry (LC-MS/MS) offering a combination of high sensitivity, exceptional mass accuracy, mass resolution, dynamic range, and detection power. Purchase of this state-of-the-art LC-MS/MS instrumentation for posttranslational modification (PTM) site mapping, protein-protein interactions, and protein identification studies is critically important to the: 1) continued success of current research programs that address fundamental questions related to diverse topics, including abiotic stress in plants, calcium signaling, smooth muscle structure, and regulation of chloride channels; 2) the ability of the university to attract new cutting-edge research programs, and 3) provide graduate students training in the area of Mass Spec technology. The NPC is a statewide core facility serving the entire Nevada System of Higher Education (NSHE) and is the only facility on either of the two major university campuses within Nevada offering proteomic analysis. The center provides proteomic and mass-spectrometry services to a large number of well-funded biomedical, biochemistry, and biotechnology oriented research programs at the University of Nevada-Reno (UNR), - Las Vegas (UNLV), and the Nevada Cancer Institute (NCI). In the past two years the Center has analyzed more than 37 thousand samples submitted by 42 NSHE faculty members, 18 external academic faculty users and 7 commercial users. Based on the number of research groups, the ever increasing demand for LC-MS/MS services, and the increasing importance of MS technologies to biomedical and biological research, it is clear that the increased capacity and new capabilities of the new instrument is critically important to the continued competitiveness and success of research programs at UNR and other Nevada institutions. In aggregate, the proposed instrument will facilitate and allow expansion of research and training projects currently garnering in excess of $56 million. PUBLIC HEALTH RELEVANCE: The proposed instrumentation will facilitate NIH supported research within the Nevada System of Higher Education (NSHE). This research includes: 1) Smooth muscle physiology looking at diabetic gastroparesis, ulcerative colitis, Cardiac disease and Muscular Dystrophy 2) Neuronal growth and Neuron reproducibility 3) Cancer Research examining Tumor cell death and Senescence 4) Stem cell plasticity.

描述(由申请者提供)：为内华达州蛋白质组学中心(NPC)购买QSTAR Elite混合LC-MS/MS质谱仪和TEMPO纳米多维液相色谱(MDLC)系统需要资金。所要求的仪器是用于在线液-质联用(LC-MS/MS)的电喷雾接口仪器，具有高灵敏度、高质量准确度、质量分辨率、动态范围和检测能力的组合。购买这种最先进的LC-MS/MS仪器用于翻译后修饰(PTM)位点图、蛋白质-蛋白质相互作用和蛋白质鉴定研究，对于以下方面至关重要：1)当前解决与不同主题相关的基本问题的研究项目继续成功，包括植物中的非生物胁迫、钙信号、平滑肌肉结构和氯离子通道的调节；2)大学吸引新的尖端研究项目的能力；以及3)在质谱学技术领域提供研究生培训。NPC是为整个内华达州高等教育系统(NHE)服务的全州核心设施，也是内华达州两个主要大学校园中唯一提供蛋白质组分析的设施。该中心为内华达大学雷诺分校(UNR)、拉斯维加斯分校(UNLV)和内华达癌症研究所(NCI)大量资金充足的生物医学、生物化学和生物技术研究项目提供蛋白质组和质谱学服务。在过去的两年里，该中心分析了42名NHE教职员工、18名外部学术教职员工和7名商业用户提交的3.7万多份样本。根据研究小组的数量、对LC-MS/MS服务的不断增长的需求以及MS技术对生物医学和生物研究的日益重要性，很明显，新仪器的能力和新能力的增加对联合国大学和内华达州其他机构的研究项目的持续竞争力和成功至关重要。总体而言，拟议的文书将便利和允许扩大目前获得5600万美元以上的研究和培训项目。公共卫生相关性：拟议的仪器将促进内华达州高等教育系统(NHE)内由NIH支持的研究。这项研究包括：1)研究糖尿病胃轻瘫、溃疡性结肠炎、心脏病和肌营养不良症的平滑肌生理学；2)神经元生长和神经元重复性；3)检测肿瘤细胞死亡和衰老的癌症研究；4)干细胞可塑性。