LTQ Orbitrap Hybrid FT Mass Spectrometry System for Proteomic Analysis

描述（申请人提供）：本申请的目标是获得一台具有高质量准确度和高分辨率的高灵敏度质谱仪，用于复杂生物样本的蛋白质组分析。该仪器需要识别和定量后修饰的氨基酸，序列生物肽，并显着提高我们的信心，确定肽或蛋白质的基础上，一个单一的串联质谱。质谱仪将通过纳米电喷雾电离源耦合到纳升流动液相色谱系统，以从各种生物样品中分离和分析低丰度肽或蛋白质。nanoLC-质谱系统将位于范德比尔特大学的质谱资源中心，主要支持NIH支持的实验室对高端质谱的日益增长的需求。该应用旨在通过为参与生物医学研究的研究实验室提供额外的高端质谱技术来利用和利用范德比尔特大学巨大的蛋白质组学专业知识。正在调查的研究项目包括免疫反应、癌症生物学、病毒发病机制、疫苗开发、生物标志物发现、转录调节、RNA加工和翻译控制。公共卫生相关性：蛋白质组学在理解包括癌症、炎症、关节炎和糖尿病在内的许多人类疾病的病因方面发挥着越来越重要的作用。所要求的LTQ Orbitrap混合FT质谱系统将为NIH资助的研究各种生物医学研究问题的研究小组提供高端质谱。正在研究的项目包括免疫反应、癌症生物学、病毒发病机制、疫苗开发、生物标志物发现、转录调控、RNA加工和翻译控制。

DESCRIPTION (provided by applicant): The goal of this application is to acquire a highly sensitive mass spectrometer with high mass accuracy and high resolution for the proteomic analysis of complex biological samples. The instrument is needed to identify and quantify posttranslationally modified amino acids, sequence biological peptides, and to improve significantly our confidence in identifying a peptide or protein based on a single tandem mass spectrum. The mass spectrometer will be coupled to a nanoliter-flow liquid chromatography system via a nanoelectrospray ionization source to fractionate and analyze low-abundance peptides or proteins from a variety of biological samples. The nanoLC-mass spectrometry system will be located in Vanderbilt University's Mass Spectrometry Resource Center to primarily support the increasing demands for high-end mass spectrometry from NIH- supported laboratories. This application seeks to capitalize and leverage the tremendous proteomics expertise at Vanderbilt University by providing additional access to high-end mass spectrometry technology to research laboratories involved in biomedical research. Research projects under investigation include the immune response, cancer biology, viral pathogenesis, vaccine development, biomarker discovery, transcriptional regulation, RNA processing, and translational control. PUBLIC HEALTH RELEVANCE: Proteomics is playing an increasingly important role in understanding the causes of many human diseases including cancer, inflammation, arthritis, and diabetes. The requested LTQ Orbitrap hybrid FT mass spectrometry system will provide access to high-end mass spectrometry for NIH-funded research groups studying a variety of biomedical research issues. Projects under investigation include the immune response, cancer biology, viral pathogenesis, vaccine development, biomarker discovery, transcriptional regulation, RNA processing, and translational control.