FEI Transmission Electron Microscope with Tomography Capability

具有断层扫描功能的 FEI 透射电子显微镜

• 批准号: 7596130
• 负责人: PAUL W. SPEARMAN
• 金额: $ 50万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7596130
• 关键词: Amyloid; Amyloid Proteins; Area; Arts; Biocompatible Materials; Biosensor; Core Facility; Development; Drug Delivery Systems; Ebola virus; Electron Microscope; Electron Microscopy; Ensure; Foundations; Funding; Generations; HIV vaccine; Housing; Image; Laboratories; Maintenance; Membrane Proteins; Microscope; Minority; Nanotechnology; Productivity; Proteins; Public Health; Request for Applications; Research; Research Infrastructure; Research Personnel; Research Project Grants; Resolution; Retroviridae; Services; Techniques; Time; United States National Institutes of Health; Universities; Virus-like particle; base; instrument; instrumentation; nanoparticle; novel; novel virus; protein folding; public health relevance; success; tomography; transmission process; vaccine development; virology

DESCRIPTION (provided by applicant): There is an urgent need on the Emory University campus for a state-of-the-art TEM to serve a multi-user group of investigators. The University has recently re-organized its core laboratories housing electron microscopes into a single shared facility, the Integrated Electron Microscopy Core. An FEI Tecnai G2 TEM is requested as a major foundation of this new core facility. The major objective of this application is to enhance EM instrumentation on the Emory campus in order to facilitate a broad array of NIH- funded research projects. This instrument will be used extensively by a group of six investigators whose projects require advanced TEM techniques. The instrument will be the only one on campus with a high resolution CCD, remote image viewing capability, and the capacity for tomography. The projects of the major users include studies of basic retrovirus assembly (Spearman and Hunter), development of novel virus-like particles with altered membrane proteins as vaccines for HIV, and Ebola virus (Compans), examination of the mechanisms of amyloid aggregate formation (Lynn), development and analysis of novel nanoparticles as biosensors and drug delivery vehicles (Nie), and synthesis of novel protein-based biomaterials (Conticello). Each major user will utilize the TEM capabilities of the FEI microscope, and will explore new applications that require tomography. A large number of core instrumentation users across campus will have access to the FEI instrument under the guidance of the core technical director for the minority of usage time not taken by the major users. The impact of this instrument on research productivity is likely to be quite large, and a strong infrastructure is already in place to ensure maintenance and technical support for the instrument. This instrument is essential to an ongoing upgrade to the EM services available at Emory University, and will support a wide base of investigators on campus. Public Health Relevance: A number of projects on the Emory University campus rely on electron microscopy for their success. This application requests a new instrument that will greatly facilitate research in the areas of virology, vaccine development, nanotechnology, amyloid protein folding, and generation of new biomaterials. The new microscope will benefit these projects and a wide range of other projects on campus that will yield benefits to public health.

描述（由申请人提供）：埃默里大学校园迫切需要一台最先进的 TEM 来为多用户研究人员群体提供服务。该大学最近将其装有电子显微镜的核心实验室重组为一个共享设施，即集成电子显微镜核心。需要使用 FEI Tecnai G2 TEM 作为这一新核心设施的主要基础。该应用程序的主要目标是增强埃默里大学校园的电磁仪器，以促进 NIH 资助的一系列广泛的研究项目。该仪器将由六名研究人员组成的小组广泛使用，他们的项目需要先进的 TEM 技术。该仪器将是校园内唯一一台具有高分辨率 CCD、远程图像查看功能和断层扫描功能的仪器。主要用户的项目包括基本逆转录病毒组装的研究（Spearman和Hunter）、开发具有改变膜蛋白的新型病毒样颗粒作为HIV和埃博拉病毒的疫苗（Compans）、淀粉样蛋白聚集体形成机制的检查（Lynn）、作为生物传感器和药物输送载体的新型纳米颗粒的开发和分析（Nie）以及新型基于蛋白质的合成 生物材料（康蒂塞洛）。每个主要用户都将利用 FEI 显微镜的 TEM 功能，并将探索需要断层扫描的新应用。校园内大量核心仪器用户将在核心技术总监的指导下使用FEI仪器，以获取主要用户未占用的少数使用时间。该仪器对研究生产力的影响可能相当大，并且强大的基础设施已经到位，以确保该仪器的维护和技术支持。该仪器对于埃默里大学现有的 EM 服务的持续升级至关重要，并将为校园内广泛的研究人员提供支持。公共卫生相关性：埃默里大学校园的许多项目都依赖电子显微镜才能取得成功。该应用需要一种新仪器，以极大地促进病毒学、疫苗开发、纳米技术、淀粉样蛋白折叠和新型生物材料生成等领域的研究。新的显微镜将使这些项目以及校园内的其他一系列项目受益，从而为公众健康带来好处。

项目成果

Monoethanolamine-induced glucose deprivation promotes apoptosis through metabolic rewiring in prostate cancer.

• DOI: 10.7150/thno.62724
• 发表时间: 2021
• 期刊: Theranostics
• 影响因子: 12.4
• 作者: Garlapati C;Joshi S;Turaga RC;Mishra M;Reid MD;Kapoor S;Artinian L;Rehder V;Aneja R
• 通讯作者: Aneja R

Twitchin kinase inhibits muscle activity.

• DOI: 10.1091/mbc.e16-10-0707
• 发表时间: 2017-06-15
• 期刊: Molecular biology of the cell
• 影响因子: 3.3
• 作者: Matsunaga Y;Hwang H;Franke B;Williams R;Penley M;Qadota H;Yi H;Morran LT;Lu H;Mayans O;Benian GM
• 通讯作者: Benian GM

Long-term survival and regeneration of neuronal and vasculature cells inside the core region after ischemic stroke in adult mice.

• DOI: 10.1111/bpa.12425
• 发表时间: 2017-07
• 期刊: Brain pathology (Zurich, Switzerland)
• 作者: Jiang MQ;Zhao YY;Cao W;Wei ZZ;Gu X;Wei L;Yu SP
• 通讯作者: Yu SP

Influenza virus exploits tunneling nanotubes for cell-to-cell spread.

• DOI: 10.1038/srep40360
• 发表时间: 2017-01-06
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Kumar A;Kim JH;Ranjan P;Metcalfe MG;Cao W;Mishina M;Gangappa S;Guo Z;Boyden ES;Zaki S;York I;García-Sastre A;Shaw M;Sambhara S
• 通讯作者: Sambhara S

Regulation of therapeutic hypothermia on inflammatory cytokines, microglia polarization, migration and functional recovery after ischemic stroke in mice.

• DOI: 10.1016/j.nbd.2016.09.013
• 发表时间: 2016-12
• 期刊: NEUROBIOLOGY OF DISEASE
• 影响因子: 6.1
• 作者: Lee, Jin Hwan;Wei, Zheng Z.;Cao, Wenyuan;Won, Soonmi;Gu, Xiaohuan;Winter, Megan;Dix, Thomas A.;Wei, Ling;Yu, Shan Ping
• 通讯作者: Yu, Shan Ping