Illumina Genome Analyzer II

Illumina 基因组分析仪 II

• 批准号: 7595701
• 负责人: XIANG-DONG FU
• 金额: $ 50万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-02-15 至 2010-02-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7595701
• 关键词: Address; Base Sequence; California; Cancer Biology; Chromosomal Rearrangement; Comprehensive Cancer Center; Core Facility; DNA; Development; Elements; Functional RNA; Funding; Gene Expression; Genes; Genome; Genomics; Grant; Housing; Investigation; Laboratories; Malignant Neoplasms; Maps; NCI-Designated Cancer Center; Request for Applications; Research; System; Technology; Therapeutic; Universities; cancer prevention; digital; instrument; medical schools; public health relevance

DESCRIPTION (provided by applicant): This application requests funding for an Illumina Solexa high-throughput sequencing unit to strengthen and expand the existing the Biomedical Genomics Microarray Facility (BIOGEM) at the University of California, San Diego. BIOGEM is a campus core facility managed jointly by the School of Medicine and the Moores UCSD Cancer Center, an NCI-designated comprehensive cancer center. This application focuses on the need for a group of cancer biologists in the Center to pursue cutting-edge research in the post-genomic era. Taking advantage of the existing Solexa sequencer in a HHMI lab at UCSD, we have established several standard applications using the instrument for digital analysis of gene expression and genomic mapping of functional DNA elements. We are also developing other high-throughput sequencing-based technologies for mapping functional RNA elements, probing gene networks, and detecting gene and chromosomal rearrangements in cancers. Because of the limited access to the existing HHMI unit, we will house the Solexa in the BIOGEM facility, to allow applications of various digital technologies to specific cancer biology problems now under investigation in the laboratories of the prime users cited in the grant request. PUBLIC HEALTH RELEVANCE: This proposal seeks funding for the purchase of a Solexa instrument, the latest high- throughput sequencing system from Illumina. The instrument will be utilized by a group of UCSD cancer biologists to address an array of NIH-funded projects studying a variety of cancer-related problems, which are essential for development of strategies for cancer prevention and therapeutics.

描述（由申请人提供）：本申请要求为Illumina Solexa高通量测序单元提供资金，以加强和扩展加州大学圣地亚哥分校现有的生物医学基因组学微阵列设施（BIOGEM）。BIOGEM是由医学院和Moores UCSD癌症中心共同管理的校园核心设施，Moores UCSD癌症中心是NCI指定的综合癌症中心。该应用程序的重点是需要一组癌症生物学家在中心追求尖端研究在后基因组时代。利用UCSD HHMI实验室现有的Solexa测序仪，我们已经建立了几个标准应用程序，使用该仪器进行基因表达的数字分析和功能DNA元件的基因组作图。我们还在开发其他基于高通量测序的技术，用于绘制功能性RNA元件，探测基因网络，以及检测癌症中的基因和染色体重排。由于对现有HHMI单元的访问有限，我们将在BIOGEM设施中安置Solexa，以便将各种数字技术应用于目前在拨款申请中引用的主要用户实验室中正在调查的特定癌症生物学问题。 公共卫生相关性：该提案寻求资金用于购买Solexa仪器，这是Illumina最新的高通量测序系统。该仪器将由一组UCSD癌症生物学家用于解决一系列NIH资助的项目，研究各种癌症相关问题，这些问题对于癌症预防和治疗策略的制定至关重要。