FUNCTION AND REGULATION OF THE HUMAN SPLICING FACTOR SC35

人类剪接因子 SC35 的功能和调控

• 批准号: 7845881
• 负责人: XIANG-DONG FU
• 金额: $ 31.01万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-30 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7845881
• 关键词: Development; Disease; Family; Funding; Genetic Transcription; Genome; Grant; Heart; Histones; Mammalian Cell; Methods; Proteins; RNA Binding; RNA Processing; RNA Splicing; Regulation; Research; Research Design; Role; Site; Tail; Tissue-Specific Splicing; base; cardiogenesis; design; human SFRS2 protein; parent grant; programs; public health relevance; response

DESCRIPTION (provided by applicant): We request a competitive supplement to our existing GM grant (5RO1 GM049369-16) in response to NOT-OD-09-058. The parent grant under the title "Function and Regulation of the Human Splicing Factor SC35" was designed to study the role of SR proteins in regulated RNA processing, focusing on the following 3 specific aims: 1. Analyze the splicing program during heart development 2. Determine the functional requirement of general and tissue-specific splicing regulators in the heart 3. Study the mechanism of regulated splicing by the synergy between SR proteins and other splicing regulators The funding period of this parent grant is from 7/01/07 to 6/30/2011. We thus have two years remaining. Here we propose an extension of this project via the competitive revision mechanism. The parent grant is my first and long-lasting RO1 since becoming independent, which has been supporting our efforts in decoding the function of SR proteins as a family of RNA binding splicing factors and regulators. Our recent discovery marked a turn of this project, revealing that SR proteins have a direct role in transcriptional elongation and a recent finding in the field indicates that SR proteins can interact directly with the tail of histone 3. Based on these recent advances, we now propose to expand our current project to study the interaction of SR proteins with the genome for functional integration between transcription and RNA processing in mammalian cells. This expanded research program has a clear potential to significantly enhance our mechanistic understanding of SR proteins in development and disease. PUBLIC HEALTH RELEVANCE: The proposed project is designed to determine the role of SR proteins in defining splice site by interacting with the genome, which has potential to reveal disease mechanisms.

描述（由申请人提供）：我们要求对我们现有的GM赠款（5RO1 GM049369-16）进行竞争补充，以回应NOT-OD-09-058。父母的授予标题为“人类剪接因子SC35的功能和调节”旨在研究SR蛋白在受调节的RNA处理中的作用，重点关注以下3个具体目的：1。在心脏发展过程中分析剪接程序2。确定一般和组织特定的斑点调节器在心脏中的跨度和组织的功能需求。监管机构该父母赠款的资金期限为从7/01/07到2011年6月30日。因此，我们还剩两年。在这里，我们通过竞争性修订机制提出了该项目的扩展。自独立以来，父母的赠款是我的第一个且持久的RO1，它一直支持我们在解码SR蛋白作为RNA结合剪接因子和调节剂家族的功能方面的努力。我们最近的发现标志着该项目的转变，表明SR蛋白在转录伸长率中具有直接的作用，并且在现场的最新发现表明，SR蛋白可以直接与组蛋白3的尾部相互作用。根据这些最新进展，我们现在建议扩大我们当前的项目，以扩大我们当前的项目，以研究SR蛋白与SR蛋白与转型的相互作用，以进行转型和RANM NANNANNA的功能和RANN NANA之间的启动和RANN NANNA之间的功能。这项扩展的研究计划具有明显的潜力，可以显着增强我们对发育和疾病中SR蛋白的机械理解。 公共卫生相关性：拟议的项目旨在通过与基因组相互作用来确定SR蛋白在定义剪接位点的作用，该基因组有可能揭示疾病机制。