High Resolution MRI Mapping of CNS Plasticity

拟议研究的长期目标是开发、验证和传播一套可推广的 非侵入性绘制功能和结构塑性变化的时空动态图的方法 在灵长类中枢神经系统中使用核磁共振。它概述了一种改进测量中枢神经系统可塑性的MRI方法的策略， 以及允许高分辨率MRI观察将局部和细胞内的变化联系起来的工具 一方面是电生理特性，另一方面是分布式大脑回路的可塑性变化， 提供了一座跨越不同尺度的方法桥梁。具体工具包括优化、翻译、mr 多尺度结构和功能脑成像方法及高精度(亚毫米)方法 用于磁共振成像和多通道受试者内和受试者间共同配准。这些方法将被开发和测试 使用具有良好特性的非人类灵长类动物模型，并将使用“黄金标准”高分辨率进行验证 标测工具包括本征信号的功能光学成像(OIS)、密集阵列电极 测绘和组织组织学。考虑到这些目标，该提案的具体目标是： 目的1)提炼和测试MRI在绘制和表征功能和结构方面的界限 完整动物体内亚毫米分辨率的可塑性。 目标2)开发综合成像和后处理工具，以实现高精度的配准 同一动物的MR图像的纵向和跨多个模式。

The long term goal of the proposed research is to develop, validate and disseminate a set of generalizable methods for non-invasive mapping of the spatio-temporal dynamics of functional and structural plastic changes in the primate CNS using MRI. It outlines a strategy for improving MRI methods for measuring CNS plasticity, together with tools that allow the high resolution MRI observations to link changes in local and cellular electrophysiological properties on the one hand, to plastic changes in distributed brain circuits on the other, providing a methodological bridge across scales. The specific tools include optimized, translational, MR approaches for multiscale structural and functional brain imaging and high precision (sub-millimeter) methods for MRI and multi-modality intra- and inter-subject co-registration. The methods will be developed and tested using a well-characterized non-human primate model, and will be validated using “gold standard” highresolution mapping tools including functional optical imaging of intrinsic signal (OIS), dense array electrode mapping, and tissue histology. With these goals in mind, the proposal’s Specific Aims are: Aim 1) To refine and test the limits of MRI for mapping and characterizing functional and structural plasticity with sub-millimeter resolution in intact animals. Aim 2) To develop integrated imaging and post-processing tools that allow high precision coregistration of MR images in the same animal longitudinally and across multiple modalities.