High Resolution MRI Mapping of CNS Plasticity

拟议研究的长期目标是开发、验证和传播一套可推广的 功能和结构塑性变化的时空动态的非侵入性映射方法 在灵长类动物中枢神经系统中的位置它概述了一种改进MRI方法测量CNS可塑性的策略， 以及允许高分辨率MRI观察将局部和细胞变化联系起来的工具， 电生理学特性，另一方面，分布在大脑回路中的可塑性变化， 提供了一个跨越尺度的方法桥梁。具体的工具包括优化、平移、MR 用于多尺度结构和功能脑成像的方法和高精度（亚毫米）方法 用于MRI和多模态受试者内和受试者间配准。这些方法将得到发展和测试 使用特征良好的非人类灵长类动物模型，并将使用"金标准"高分辨率进行验证 标测工具，包括内在信号的功能光学成像（OIS）、密集阵列电极 映射和组织组织学。考虑到这些目标，该提案的具体目标是： 目的1）完善和测试MRI在功能和结构标测和表征方面的局限性 可塑性与亚毫米分辨率在完整的动物。 目标2）开发集成的成像和后处理工具，以实现高精度的配准 同一动物的MR图像纵向和跨多种模态。

The long term goal of the proposed research is to develop, validate and disseminate a set of generalizable methods for non-invasive mapping of the spatio-temporal dynamics of functional and structural plastic changes in the primate CNS using MRI. It outlines a strategy for improving MRI methods for measuring CNS plasticity, together with tools that allow the high resolution MRI observations to link changes in local and cellular electrophysiological properties on the one hand, to plastic changes in distributed brain circuits on the other, providing a methodological bridge across scales. The specific tools include optimized, translational, MR approaches for multiscale structural and functional brain imaging and high precision (sub-millimeter) methods for MRI and multi-modality intra- and inter-subject co-registration. The methods will be developed and tested using a well-characterized non-human primate model, and will be validated using “gold standard” highresolution mapping tools including functional optical imaging of intrinsic signal (OIS), dense array electrode mapping, and tissue histology. With these goals in mind, the proposal’s Specific Aims are: Aim 1) To refine and test the limits of MRI for mapping and characterizing functional and structural plasticity with sub-millimeter resolution in intact animals. Aim 2) To develop integrated imaging and post-processing tools that allow high precision coregistration of MR images in the same animal longitudinally and across multiple modalities.