HOPKINS DIGESTIVE DISEASE BASIC RESEARCH DEVELOPMENT CENTER

霍普金斯消化疾病基础研究开发中心

• 批准号: 7868611
• 负责人: MARK DONOWITZ
• 金额: $ 10.59万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-25 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7868611
• 关键词: Basic Science; Binding; Bioinformatics; Blood; Budgets; Collaborations; Collection; Communication; Consultations; Digestive System Disorders; Disease; Epithelial; Epithelial Cells; Equipment; Feces; Fostering; Genotype; Human Resources; Image; Instruction; Intestines; Investments; Kidney; Lasers; Liquid Chromatography; Liver; Liver diseases; Maps; Mass Spectrum Analysis; Metabolic; Microscopy; Mus; National Institute of Diabetes and Digestive and Kidney Diseases; Occupational activity of managing finances; Operative Surgical Procedures; Organ; Pancreas; Perfusion; Physiology; Post-Translational Protein Processing; Preparation; Process; Proteins; Proteomics; Quality Control; Research; Research Personnel; Site; Source; Tissues; Universities; Urine; design; digital; gastrointestinal; light microscopy; medical schools; member; mouse model; programs; protein degradation; research and development; two-photon; voltage clamp

DESCRIPTION, OVERALL (provided by applicant): The Hopkins Digestive Diseases Basic Research Development Core Center is an NIDDK Research Development Center (DDBRDCC) which has been designed to advance basic science and translational digestive diseases research at the Johns Hopkins University School of Medicine and to foster collaborations and new directions of digestive (gastrointestinal and liver) diseases research by providing access for its members and associate members to Cores: A. Epithelial Proteomics Core; B. Imaging Core; C. Mouse Physiology Core. The DDBRDCC is a partnership between the JHUSOM and the members of this Center, with investment in personnel and equipment by the JHUSOM supplementing the Center budget. Core A, Epithelial Proteomics Core will help identify proteins that are differentially expressed in Gl tract/liver/kidney normally or in diseases of these organs, track interactions among proteins including identification of binding partners, examine protein turnover, map cleavage sites and determine post-translational modifications. The approaches will use 2D and liquid chromatography separations and mass spectrometry. This Core will provide a technician to interface with Core investigators for planning, quality control, and bioinformatics consultation. Core B, Imaging Core will make multiple types of cutting edge imaging and EM of epithelial cells available to DDBRDCC investigators and provide a Core Manager to help perform the studies. These include confocal and two-photon microscopy; use of a fluorometer, digital camera; and access to laser capture microscopy. Core B will also assist DDBRDCC investigators prepare intestine, liver, pancreas and kidney for EM. Core C, Mouse Physiology Core will provide advice in establishing mouse colonies, genotype mouse models of digestive diseases, make available metabolic cages and perform urine, stool, blood collections and analysis. This Core will also perform aortic perfusion of mice as part of intestinal, liver, pancreas, kidney procurement and preparation for Northern and Western analysis. This approach will also be used by Core C to remove, orient, fix, process, embed, section and mount these tissues to be used for light microscopy. Core C will make equipment available and instruction in its use for Ussing chamber/voltage clamp/active intestinal Na+ and Cl- transport studies. Administrative Operations will provide the communication, financial management and administrative functions of the DDBRDCC and organize the enrichment program (financed by non-Core sources).

总体描述(由申请人提供)：霍普金斯消化疾病基础研究发展核心中心是NIDDK研究发展中心(DDBRDCC)，旨在促进约翰霍普金斯大学医学院的基础科学和转化性消化疾病研究，并通过为其成员和准成员提供访问核心来促进消化(胃肠和肝脏)疾病研究的合作和新方向：A.上皮蛋白质组学核心；B.成像核心；C.小鼠生理学核心。DDBRDCC是JHUSOM和本中心成员之间的伙伴关系，JHUSOM在人员和设备方面的投资补充了中心的预算。核心A，上皮蛋白质组学核心将帮助识别在胃肠道/肝脏/肾脏正常或在这些器官的疾病中差异表达的蛋白质，追踪蛋白质之间的相互作用，包括识别结合伙伴，检查蛋白质周转，绘制切割位点图，并确定翻译后修饰。这些方法将使用2D和液相色谱分离以及质谱分析。这个核心将提供一名技术人员与核心调查人员进行沟通，以进行规划、质量控制和生物信息学咨询。核心B，成像核心将为DDBRDCC调查人员提供多种类型的上皮细胞的尖端成像和EM，并提供一名核心经理来帮助执行研究。这包括共焦显微镜和双光子显微镜；荧光计、数码相机的使用；以及激光捕获显微镜的使用。CORE B还将协助DDBRDCC调查人员为EM准备肠道、肝脏、胰腺和肾脏。核心C，小鼠生理学核心将在建立小鼠群体、为消化疾病的小鼠模型进行基因分型、提供代谢笼以及进行尿液、粪便、血液采集和分析方面提供建议。该核心还将对小鼠进行主动脉灌注，作为肠道、肝脏、胰腺、肾脏采集和Northern和Western分析准备的一部分。Core C还将使用这种方法来移除、定位、固定、加工、嵌入、切片和安装这些用于光学显微镜的组织。CORE C将为Ussing小室/电压钳/活性肠道Na+和Cl-转运研究提供设备和使用指导。行政业务将提供DDBRDCC的通信、财务管理和行政职能，并组织浓缩计划(由非核心来源提供资金)。