Initial Biomedical Study of Turbidity Suppression by Optical Phase Conjugation

描述（由申请人提供）：本探索性/开发性研究（R21）申请旨在研究浊度抑制光学相位共轭（TS-OPC）现象，并探索其在生物医学应用中的用途。TS-OPC-我的小组最近观察到的一种生物光子现象，是基于使用光学相位共轭来抑制组织混浊。与吸收不同，弹性光散射是时间可逆过程。因此，散射场的时间反转副本应该能够通过散射介质追溯其轨迹并恢复原始光场。我们最初的实验表明，这种现象是令人惊讶的强大-我们能够观察到的现象与鸡组织切片的厚度为5毫米和波长为532纳米。我们的初步调查还指出该方法在以下几个应用中的潜在实用性：微型皮下植入物的光动力，改进光动力治疗程序，组织吸收/散射询问等。拟议研究的重点是更全面地了解该技术的工作原理，并开始对其用于光动力学应用中增强和靶向光递送到组织中的用途进行初步研究。本研究的具体目的是：1.开发一个快速、高效、简单的反射式TS-OPC系统。2.实验确定反射模式TS-OPC方法的限制。3.建立一个理论框架来理解TS-OPC方法。4.开始对使用TS-OPC增强和靶向组织的光传递进行初步研究-用于光动力学治疗（PDT）。光学相位共轭项目的叙述公共卫生相关性：通过光学相位共轭抑制浊度的现象可能适用于临床诊断和治疗。拟议研究的资金将使我们能够更好地了解这一新发现的现象，并使我们能够开始探索其更有前途的应用之一-在光动力治疗过程中增强光输送。

DESCRIPTION (provided by applicant): This Exploratory/Developmental Research (R21) application proposes to study the phenomenon of turbidity suppression optical phase conjugation (TS-OPC) and explore its uses for biomedical applications. TS-OPC - a biophotonic phenomenon recently observed by my group, is based on the use of optical phase conjugation to suppress tissue turbidity. Unlike absorption, elastic optical scattering is a time reversible process. As such, a time reversed copy of the scattered field should be able to retrace its trajectory through the scattering medium and recover the original light field. Our initial experiments revealed that this phenomenon is surprisingly robust - we were able to observe the phenomenon with a chicken tissue section of thickness 5mm and a wavelength of 532 nm. Our initial investigations also point to the potential utility of the method for several applications: optical powering of miniature sub-dermal implants, improving photodynamic therapy procedures, tissue absorption/scattering interrogation, etc. The focus of the proposed research is to attain a more complete understanding of the technique's working principle, streamline its implementation in reflection mode and begin initial investigations into its use for enhanced and targeted light delivery into tissues for photodynamic applications. The specific aims of the proposed research are: 1. Develop a quick, efficient and simple reflection mode TS-OPC system. 2. Experimentally determine the limits of the reflection mode TS-OPC method. 3. Develop a theoretical framework for understanding the TS-OPC method. 4. Begin initial investigations into the use of TS-OPC for enhanced and targeted light delivery into tissues - useful for photodynamics therapy (PDT). Narrative for Optical phase conjugation project PUBLIC HEALTH RELEVANCE: The phenomenon of turbidity suppression via optical phase conjugation can potentially be adapted for clinical diagnosis and therapeutics. The funding of the proposed research will allow us to better understand this newly discovered phenomenon and allow us to begin exploring one of its more promising applications - the enhancement of light delivery during photodynamic therapy procedures.