Epigenetics Core

表观遗传学核心(核心B)的主要目标是为每个项目负责人及其团队提供我们的知识、资源和技术支持，以剖析导致内皮细胞(ECs)适当的时间和空间分化的表观遗传学事件。核心已经开发并实施了每个项目负责人成功所需的技术。每名调查员都将接受必要的礼仪和技术培训，以开展他们的实验。例如，我们将帮助每个研究小组为ChlP芯片实验建立最佳的ChlP条件。该核心将提供必要的控制和帮助优化、杂交和分析平铺微阵列。我们与西肯塔基大学生物信息学和信息科学中心的长期合作关系将促进对这些方法产生的数据的快速分析。核心还将提供技术支持和定量实时PCR机 对于先前识别的靶基因以及新发现的与EC分化有关的基因靶点的高分辨率ChlP验证。该核心目前正在使用罗氏/NimbleGen的高密度微阵列进行表达和ChlP-ChIP。我们还在使用Agilent/Invitgen非编码微阵列(即长非编码和microRNAs)。这些基因组方法使我们能够确定组蛋白去甲基酶下游的编码和非编码靶标。来自我们研究的观察结果将不可避免地为这个项目中提出的问题提供有价值的见解。这一核心的最终目标是为发现调控内皮细胞生物学的表观遗传学机制提供技术和科学监督；促进全基因组实验的快速发展和解释；并将我们对组蛋白修饰动力学的总体兴趣与对人类健康和疾病中调控内皮细胞命运和分化的分子机制的理解结合起来。

The primary goal of the Epigenetics Core (Core B) is to provide each project leader and their team access to our knowledge, resources, and technical support for dissecting apart the epigenetic events that are responsible for the proper temporal and spatial differentiation of endothelial cells (ECs). The core has developed and implemented the techniques that are required for each project leader to be successful. Each investigator will be provided with the necessary protocols and technical training to carry out their experiments. For example, we will help each investigator's team establish optimal ChlP conditions for the ChlP-chip experiments. The core will provide the necessary controls and help with the optimization, hybridizations, and analysis of tiling microarrays. Our longstanding relationship with the Bioinformatics and Information Science Center at Western Kentucky University will facilitate rapid analysis of the data generated by these approaches. The core will also provide technical support and quantitative real-time PCR machines for the high resolution ChlP validation of previously identified target genes as well as the newly discovered gene targets involved in EC differentiation. The core is currently using high density microarrays from Roche/NimbleGen for expression and ChlP-chip. We are also using Agilent/lnvitrogen non-coding microarrays {i.e., Long Non-coding and MicroRNAs). These genomic approaches have allowed us to identify the coding and non-coding targets that are downstream of histone demethylases. The observations from our studies will inevitably provide valuable insights into the questions being asked in this program project. The ultimate goal of this core is to provide technical and scientific oversight for the discovery oif epigenetic mechanisms regulating endothelial biology; to facilitate rapid development and interpretation of genome-wide experiments; and to integrate our overall interest in histone modification dynamics with understanding the molecular mechanisms regulating endothelial cell fate and differentiation in human health and disease.