Epigenetics Core

表观遗传学核心

• 批准号: 8001275
• 负责人: William C Aird
• 金额: $ 38.38万
• 依托单位: BETH ISRAEL DEACONESS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2015-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8001275
• 关键词: Area; Bioinformatics; Biological; Biology; Cell Differentiation process; Code; Cues; Data; Data Analyses; Development; Disease; Endothelial Cells; Epigenetic Process; Event; Functional RNA; Gene Targeting; Genes; Genomics; Goals; Health; Heterogeneity; Histones; Human; Information Resources; Information Sciences; Kentucky; MicroRNAs; Molecular; Phenotype; Process; Protocols documentation; Research Personnel; Resolution; Resources; Technical Expertise; Techniques; Time; Training; Universities; Validation; density; gene discovery; genome-wide; histone modification; insight; interest; novel; postnatal; programs; research study; response

The primary goal of the Epigenetics Core (Core B) is to provide each project leader and their team access to our knowledge, resources, and technical support for dissecting apart the epigenetic events that are responsible for the proper temporal and spatial differentiation of endothelial cells (ECs). The core has developed and implemented the techniques that are required for each project leader to be successful. Each investigator will be provided with the necessary protocols and technical training to carry out their experiments. For example, we will help each investigator's team establish optimal ChlP conditions for the ChlP-chip experiments. The core will provide the necessary controls and help with the optimization, hybridizations, and analysis of tiling microarrays. Our longstanding relationship with the Bioinformatics and Information Science Center at Western Kentucky University will facilitate rapid analysis of the data generated by these approaches. The core will also provide technical support and quantitative real-time PCR machines for the high resolution ChlP validation of previously identified target genes as well as the newly discovered gene targets involved in EC differentiation. The core is currently using high density microarrays from Roche/NimbleGen for expression and ChlP-chip. We are also using Agilent/lnvitrogen non-coding microarrays {i.e., Long Non-coding and MicroRNAs). These genomic approaches have allowed us to identify the coding and non-coding targets that are downstream of histone demethylases. The observations from our studies will inevitably provide valuable insights into the questions being asked in this program project. The ultimate goal of this core is to provide technical and scientific oversight for the discovery oif epigenetic mechanisms regulating endothelial biology; to facilitate rapid development and interpretation of genome-wide experiments; and to integrate our overall interest in histone modification dynamics with understanding the molecular mechanisms regulating endothelial cell fate and differentiation in human health and disease.

表观遗传学核心（核心B）的主要目标是为每个项目负责人及其团队提供我们的知识，资源和技术支持，以解剖负责内皮细胞（EC）适当的时间和空间分化的表观遗传事件。核心团队已经开发并实施了每个项目领导者成功所需的技术。将向每名研究人员提供进行实验所需的方案和技术培训。例如，我们将帮助每个研究者的团队建立用于ChIP芯片实验的最佳ChIP条件。核心将提供必要的控制和帮助的优化，杂交和分析平铺微阵列。我们与西肯塔基州大学生物信息学和信息科学中心的长期合作关系将促进对这些方法产生的数据的快速分析。核心还将提供技术支持和定量实时PCR机器 用于先前鉴定的靶基因以及新发现的参与EC分化的基因靶的高分辨率ChIP验证。该核心目前使用来自Roche/NimbleGen的高密度微阵列用于表达和ChIP芯片。我们还使用Agilent/Invitrogen非编码微阵列（即，长非编码和微RNA）。这些基因组学方法使我们能够识别组蛋白去甲基化酶下游的编码和非编码靶标。从我们的研究观察将不可避免地提供宝贵的见解被问到这个程序项目中的问题。该核心的最终目标是为发现调节内皮生物学的表观遗传机制提供技术和科学监督;促进全基因组实验的快速发展和解释;并将我们对组蛋白修饰动力学的整体兴趣与理解调节人类健康和疾病中内皮细胞命运和分化的分子机制相结合。