EFFECT OF HYDROGEL ENCAPSULATION ON THE NEURONAL DIFFERENTIATION OF MES CELLS

水凝胶封装对 MES 细胞神经元分化的影响

• 批准号: 8168144
• 负责人: Eduardo Martinez-Ceballos
• 金额: $ 5.55万
• 依托单位: LOUISIANA STATE UNIV A&M COL BATON ROUGE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-15 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168144
• 关键词: Cell Aggregation; Cell Death; Cells; Computer Retrieval of Information on Scientific Projects Database; Diffusion; Ectoderm; Encapsulated; Endoderm; Funding; Future; Generations; Germ Layers; Grant; Hydrogels; Institution; Mesoderm; Methods; Microgravity; Neuronal Differentiation; Nutrient; Procedures; Research; Research Personnel; Resources; Seeds; Source; Testing; Therapeutic; United States National Institutes of Health; base; cell type; embryonic stem cell; scaffold; stem cell differentiation; wasting

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Embryonic stem (ES) cells are pluripotent cells that can differentiate into all three main germ layers: endoderm, mesoderm, and ectoderm. A number of methods have been developed to differentiate ES cells into specific cell types in culture. The methods that have been primarily employed in the directed differentiation of ES cells rely on the generation of three-dimensional ES cell aggregates called embryoid bodies (EBs). One inherent limitation of this procedure is the low efficiency of ES cell differentiation due to the formation of cavitated or cystic EB. This cavitation is produced by cell death at the EB core due to a decrease in diffusion of nutrients into the deeper cell layers of the embryoids. We hypothesize that an increase in both nutrient diffusion and cellular waste into and out the EBs, respectively, may result in a high efficiency of ES cell differentiation. To test this hypothesis, we have encapsulated the ES cells inalginate-based hydrogels and examined the efficiency of neuronal differentiation after microgravity culture for 8 days. Our results indicate that encapsulation of mES cells prior to cell aggregation resulted in a 2-fold increase in neuronal differentiation efficiency as compared to that obtained from normal EBs culture. For future studies, we will seed the differentiated cells, obtained from encapsulation, into biodegradable scaffolds suitable for future therapeutic applications.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 胚胎干（ES）细胞是多能细胞，可以分化成所有三个主要胚层：内胚层、中胚层和外胚层。已经开发了多种方法来将 ES 细胞分化为培养中的特定细胞类型。主要用于 ES 细胞定向分化的方法依赖于称为胚状体 (EB) 的三维 ES 细胞聚集体的生成。该过程的一个固有限制是由于空化或囊性 EB 的形成而导致 ES 细胞分化效率低。这种空化是由于营养物质扩散到胚状体更深细胞层的减少而导致 EB 核心细胞死亡而产生的。我们假设营养物扩散和细胞废物进出 EB 的增加可能会导致 ES 细胞分化的高效率。为了验证这一假设，我们将 ES 细胞封装在基于藻酸盐的水凝胶中，并检查微重力培养 8 天后神经元分化的效率。我们的结果表明，与正常 EB 培养物相比，在细胞聚集之前封装 mES 细胞导致神经元分化效率增加 2 倍。对于未来的研究，我们将从封装中获得的分化细胞接种到适合未来治疗应用的可生物降解支架中。