In Vivo Cellular and Molecular Imaging Centers (ICMICs)

体内细胞和分子成像中心 (ICMIC)

• 批准号: 7892611
• 负责人: ROBERT F MATTREY
• 金额: $ 1.74万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7892611
• 关键词: Vivo; Cellular; Molecular; Imaging; Centers

This proposal focuses on differences in the susceptibility to genotoxicants of early and late stage embryos with respect to embryological development. Grass shrimp will be used as a model organism to characterize the difference in susceptibility of early and late stage embryos to contaminant induced DNA damage. Grass shrimp embryos have been used for toxicity testing. Adult grass shrimp will be exposed to sediments and food containing chromium, mercury, or polycyclic aromatic hydrocarbons (PAH?s). Embryo stages produced from control and genotoxicant exposed adults will be followed through the various stages of development. DNA strand breakage and repair will be measured using the comet assay, including modifications for quantification of specific lesions and crosslinks. A DNA repair enzyme will be amplified, cloned and sequenced. Based on this sequence, primers will be prepared and tested to begin studies on the expressi on of a DNA repair gene in early and late stage embryos. The significance of this work is that it provides a model for the study of genotoxicity. By working with grass shrimp embryos, which have well studied developmental processes, a quick reproductive cycle which is easily manipulated in the laboratory, and are routinely exposed to genotoxicants in contaminated environments, we can understand the processes which make early embryonic stages more sensitive to contaminants.

这一建议的重点是在胚胎发育的早期和晚期胚胎易感性的差异。草虾将被用作模型