Cellular and Molecular Physiology Core

细胞和分子生理学核心

• 批准号: 7688375
• 负责人: JAMES Lorenzen BOYER
• 金额: $ 22.19万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2014-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7688375
• 关键词: Adenovirus Infections; Adenovirus Vector; Adult; Biological; Cell Culture Techniques; Cell Line; Cell Separation; Cells; Community Health Centers; Core Facility; Development; Digestive System Disorders; Endothelial Cells; Equipment; Equus caballus; Fibroblasts; Gene Expression; Grant; Hepatic; Hepatocyte; Human; Human Resources; Image; Island; Kupffer Cells; Laboratories; Liver; Lymphocyte; Maintenance; Marines; Methods; Modeling; Molecular; Mus; Organ; Perfusion; Physiology; Preparation; Procedures; Quality Control; Raja; Rattus; Research; Research Personnel; Services; Skates; Skating; Small Interfering RNA; Suspension substance; Suspensions; Technical Expertise; Techniques; Technology; Time; Tissues; Transfection; United States National Institutes of Health; Universities; Vertebrates; Work; bile duct; cell preparation; cholangiocyte; member; monolayer; stellate cell

General Description. This Core facility is the "work horse" of this Center and is organized to provide technical expertise, equipment and personnel to Liver Center Investigators who wish to work with: a) Isolated cell preparations including: hepatocytes, cholangiocytes, endothelial cells, stellate cells, portal fibroblasts and hepatic lymphocytes, primarily from rat and mouse. Recently human hepatocytes have also been obtained, b) Cell culture facilities for short and long term cultures and cell lines, c) Isolated liver perfusion preparations for studies utilizing the whole organ, d) Gene expression using Quantitative real time PCR machine and Phosphor-imaging equipment or e) Altering gene expression in these cells and tissues using siRNA transfection and adenovirus infection technologies. By centralizing these procedures in a Core facility, investigators are assured of a high degree of quality control and preparations can often be used simultaneously by more then one investigator, f) Services a-c are also established at the Center's satellite facility at the Mt Desert Island Biological Laboratory for use of alternative models in marine species, primarily the small skate, Raja erinacea.

一般描述。该核心设施是该中心的“工作马”，其组织目的是提供技术支持， 肝脏中心研究者的专业知识、设备和人员，他们希望使用： 制备物包括：肝细胞、胆管细胞、内皮细胞、星状细胞、门静脉成纤维细胞和 肝淋巴细胞，主要来自大鼠和小鼠。最近还获得了人肝细胞，B） 用于短期和长期培养和细胞系的细胞培养设施，c）分离的肝脏灌注制剂 对于利用整个器官的研究，d）使用定量真实的时间PCR仪的基因表达，和 e）使用siRNA改变这些细胞和组织中的基因表达 转染和腺病毒感染技术。通过将这些程序集中在核心设施中， 调查人员确信有高度的质量控制， f）还在中心的卫星上建立服务a-c 沙漠岛山生物实验室的设施，用于海洋物种的替代模型，主要是 一种叫刺鳐的小冰鞋