Cellular and Molecular Physiology Core

一般描述。该核心设施是该中心的“工作机器”，旨在提供技术支持 向希望从事以下工作的肝脏中心研究人员提供专业知识、设备和人员：a)分离的细胞 制备包括：肝细胞、胆管细胞、内皮细胞、星状细胞、门静脉成纤维细胞和 肝淋巴细胞，主要来自大鼠和小鼠。最近也获得了人肝细胞，b) 用于短期和长期培养和细胞系的细胞培养设施，c)隔离肝脏灌流制剂 对于利用整个器官的研究，d)使用定量实时PCR机的基因表达和 荧光成像设备或e)使用siRNA改变这些细胞和组织中的基因表达 转染法和腺病毒感染技术。通过将这些程序集中在一个核心设施中， 调查人员被保证有高度的质量控制，并且经常可以使用制剂 同时由不止一名调查员，f)在该中心的卫星上也建立了a-c服务 荒山岛生物实验室的设施，主要用于海洋物种的替代模式 一种小型溜冰鞋--虎斑鱼。

General Description. This Core facility is the "work horse" of this Center and is organized to provide technical expertise, equipment and personnel to Liver Center Investigators who wish to work with: a) Isolated cell preparations including: hepatocytes, cholangiocytes, endothelial cells, stellate cells, portal fibroblasts and hepatic lymphocytes, primarily from rat and mouse. Recently human hepatocytes have also been obtained, b) Cell culture facilities for short and long term cultures and cell lines, c) Isolated liver perfusion preparations for studies utilizing the whole organ, d) Gene expression using Quantitative real time PCR machine and Phosphor-imaging equipment or e) Altering gene expression in these cells and tissues using siRNA transfection and adenovirus infection technologies. By centralizing these procedures in a Core facility, investigators are assured of a high degree of quality control and preparations can often be used simultaneously by more then one investigator, f) Services a-c are also established at the Center's satellite facility at the Mt Desert Island Biological Laboratory for use of alternative models in marine species, primarily the small skate, Raja erinacea.