THE RETINAL CONNECTOME

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这项研究的长期目标是：(1)为哺乳动物的视网膜生成一个完整的网络图，(2)定义所有视网膜细胞的信号特征。分子分析和计算分析的融合现在使在实际时间框架内完成这些任务成为可能。 具体目标1.实现哺乳动物视网膜的完整网络图。 视网膜网络聚集在神经节细胞上，创造了大约15-20个过滤版本的视觉世界。这些过滤器的图谱将通过融合分子表型(以可视化所有细胞)、激发映射(以可视化功能)和大规模超微结构成像(以可视化所有连接)来获得。新的策略是将分子数据计算传播到超微结构数据中，生成所有细胞类别和连接的组合图。意义：理解任何系统都需要一张完整的地图：一张可以用来衡量疾病或实验干预引发的变化的地图。这项工作具有新的重要性，因为遗传性或获得性视网膜变性现在已知会严重影响视网膜连接和神经元存活，显示出与颞叶癫痫的显著相似之处。一张完整的网络图为任何恢复视力的战略提供了必不可少的指导。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The long term goals of this research are to: (1) generate a complete network map for the mammalian retina and (2) define the signaling profiles of all retinal cells. Fusions of molecular and computational analyses now make it possible to perform these tasks in a practical time frame. Specific Aim 1. Realization of a complete network map for the mammalian retina. Retinal networks converge on ganglion cells, creating some 15-20 filtered versions of the visual world. Maps of these filters will be acquired by fusing molecular phenotyping (to visualize all cells), excitation mapping (to visualize function), and large-scale ultrastructural imaging (to visualize all connections). The novel strategy is the computational propagation of molecular data into the ultrastructural data, producing a combined map of all cell classes and connections. Significance: Understanding any system requires a complete map: a map against which changes triggered by disease or experimental intervention can be gauged. This work has taken on new importance as inherited or acquired retinal degenerations are now known to heavily impact retinal wiring and neuronal survival, showing significant similarities to temporal lobe epilepsy. A complete network map provides the essential guide for any strategy to restore vision.