Cell Biology Support

修订后的核心A简介：细胞生物学支持： 我们感谢评审员提供的评论，以及两位评审员都发现核心A 工作人员具有执行拟议目标的专业知识。因此，核心在这方面的目标 修改后的申请保持不变。审查人员提出了对每一项可用资金重叠的关切 关于细胞培养的项目，以及关于单个项目是否会有任何重叠功能的关注。 作为回应，我们已按建议修订了人员努力和供应预算，并作了一些修改，如下 在预算理由中进行了解释。关于是否与核心A有重叠功能的项目， 我们认为，无论是在人员努力还是灵活的预算方面，他们都不会。核心A和每个项目的人员 有一些共同的专业知识，但仅核心A的人员提供实际隔离的FTE， 扩增和培养所有人和所有动物的内皮细胞、所有细胞系和使用的其他细胞 程序中的研究人员，为每个特定的实验要求提供所有正确的塑料器皿格式 应项目调查人员的要求。这为项目中的调查人员提供了一个关键的优势 因为核心人员将对所有细胞、血清和生长因子进行最高标准的质量控制 以及在整个项目的体外实验中使用的所有其他必要试剂。

INTRODUCTION TO REVISED CORE A: CELL BIOLOGY SUPPORT: We appreciate the comments provided by reviewers and the fact that both reviewers found that Core A personnel have the expertise to perform the proposed objectives. Thus, the objectives of the Core in this revised application are unchanged. Reviewers raised a concern of overlap in funds available in each of the projects for cell culture, and a concern as to whether the individual projects will have any overlapping function. In response, we have revised the personnel effort and supply budget as suggested, with some modification as explained in the budget justification. In regards to whether projects having overlapping function with Core A, we believe they do not, neither in personnel effort or supple budget. Core A and the personnel in each Project have some expertise in common, but the personnel in Core A alone provide the FTEs that actually isolate, amplify and culture all human and all animal endothelial cells, all cell lines, and other cells used by investigators in the Program, in the all correct plasticware formats for each specific experimental requirement requested by the Project investigators. This provides a critical advantage to the Investigators in the Program because Core personnel will use the highest standards of quality control on all cells, serum and growth factors and all other necessary reagents used in in vitro experiments across the program.