Cell Biology Support
Cell Biology Support
批准号:
7753053
负责人:
FRANCIS W. LUSCINSKAS
金额:
$21.93万
依托单位国家:
美国
项目类别:
财政年份:
2009
资助国家:
美国
项目状态:
已结题
起止时间:
2009-07-01 至 2014-06-30
关键词:
AdenovirusesAdhesivesAnimalsBiologicalBloodBlood VesselsBudgetsCell Culture SystemCell Culture TechniquesCell LineCellsCellular biologyCultured CellsDecontaminationDermalEndothelial CellsEndotheliumEnsureEnzymesFibroblast Growth FactorFunctional disorderFundingGoalsGrowth FactorHumanHuman ResourcesIn VitroIndividualKnock-outLeukocytesMediationMedicalMethodologyModificationMusPriceProceduresProductionProteinsPublished CommentQuality ControlReagentRecombinantsResearchResearch PersonnelSerumSourceSterilityTechniquesTestingTissuesTrainingTransgenic MiceUmbilical veinVascular Endothelial CellWorkcostfetal bovine serumflasksgraduate studenthigh standardhuman tissuein vitro Modelneonatal humanneonatepenis foreskinprogramsresearch studyresponsetissue culturevector
中文摘要
修订核心A:细胞生物学支持简介:
我们感谢评审员提供的评论,以及两位评审员都发现核心A
工作人员具备实现拟议目标的专业知识。因此,核心在这方面的目标
修改后的应用程序不变。审查人员对每个项目的可用资金重叠表示关切,
此外,我们亦关注到,个别项目是否会有任何功能重叠。
作为回应,我们已按照建议修订了人员工作和供应预算,并作了一些修改,
在预算说明中解释。关于项目是否与核心A的职能重叠,
我们认为,无论是在人员努力方面,还是在灵活的预算方面,他们都没有做到这一点。核心A及各项目人员
有一些共同的专业知识,但核心A中的人员单独提供了实际隔离的FTE,
扩增和培养所有人和所有动物内皮细胞、所有细胞系和其他细胞,
研究人员在该计划中,在所有正确的塑料器皿格式为每个特定的实验要求
项目调查人员的要求。这为项目中的研究者提供了关键优势
因为核心人员将对所有细胞、血清和生长因子采用最高标准的质量控制,
以及整个项目中体外实验中使用的所有其他必要试剂。
英文摘要
INTRODUCTION TO REVISED CORE A: CELL BIOLOGY SUPPORT:
We appreciate the comments provided by reviewers and the fact that both reviewers found that Core A
personnel have the expertise to perform the proposed objectives. Thus, the objectives of the Core in this
revised application are unchanged. Reviewers raised a concern of overlap in funds available in each of the
projects for cell culture, and a concern as to whether the individual projects will have any overlapping function.
In response, we have revised the personnel effort and supply budget as suggested, with some modification as
explained in the budget justification. In regards to whether projects having overlapping function with Core A,
we believe they do not, neither in personnel effort or supple budget. Core A and the personnel in each Project
have some expertise in common, but the personnel in Core A alone provide the FTEs that actually isolate,
amplify and culture all human and all animal endothelial cells, all cell lines, and other cells used by
investigators in the Program, in the all correct plasticware formats for each specific experimental requirement
requested by the Project investigators. This provides a critical advantage to the Investigators in the Program
because Core personnel will use the highest standards of quality control on all cells, serum and growth factors
and all other necessary reagents used in in vitro experiments across the program.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
CD47 Regulation of Leukocyte Integrins during Leukocyte Trafficking
-
批准号:9105867
-
项目类别:
-
资助金额:$58.46万
-
财政年份:2016
-
负责人:FRANCIS W. LUSCINSKAS
-
依托单位:
2014 Annual Meeting of the American Society for Investigative Pathology
-
批准号:8716277
-
项目类别:
-
资助金额:$0.25万
-
财政年份:2014
-
负责人:FRANCIS W. LUSCINSKAS
-
依托单位:
2013 Annual Meeting of the American Society for Investigative Pathology
-
批准号:8527049
-
项目类别:
-
资助金额:$1.5万
-
财政年份:2013
-
负责人:FRANCIS W. LUSCINSKAS
-
依托单位:
The Role of CD47 in Mononuclear Leukocyte Recruitment
-
批准号:7753041
-
项目类别:
-
资助金额:$61.36万
-
财政年份:2009
-
负责人:FRANCIS W. LUSCINSKAS
-
依托单位:
Administrative Core
-
批准号:7753059
-
项目类别:
-
资助金额:$15.28万
-
财政年份:2009
-
负责人:FRANCIS W. LUSCINSKAS
-
依托单位:
ENDOTHELIAL LATERAL JUNCTIONS AND LEUKOCYTE TRAFFICKING
-
批准号:6602438
-
项目类别:
-
资助金额:$6.87万
-
财政年份:2002
-
负责人:FRANCIS W. LUSCINSKAS
-
依托单位:
CORE--CELL BIOLOGY SUPPORT
-
批准号:6602444
-
项目类别:
-
资助金额:$6.87万
-
财政年份:2002
-
负责人:FRANCIS W. LUSCINSKAS
-
依托单位:
ENDOTHELIAL LATERAL JUNCTIONS AND LEUKOCYTE TRAFFICKING
-
批准号:6469264
-
项目类别:
-
资助金额:$6.87万
-
财政年份:2001
-
负责人:FRANCIS W. LUSCINSKAS
-
依托单位:
CORE--CELL BIOLOGY SUPPORT
-
批准号:6469270
-
项目类别:
-
资助金额:$6.87万
-
财政年份:2001
-
负责人:FRANCIS W. LUSCINSKAS
-
依托单位:
CORE--CELL BIOLOGY SUPPORT
-
批准号:6327723
-
项目类别:
-
资助金额:$32.32万
-
财政年份:2000
-
负责人:FRANCIS W. LUSCINSKAS
-
依托单位:
ENDOTHELIAL LATERAL JUNCTIONS AND LEUKOCYTE TRAFFICKING
-
批准号:6327717
-
项目类别:
-
资助金额:$32.32万
-
财政年份:2000
-
负责人:FRANCIS W. LUSCINSKAS
-
依托单位:
ENDOTHELIAL CELL DYSFUNCTION IN CHRONIC IMMUNE DISEASES
-
批准号:6661274
-
项目类别:
-
资助金额:$26.7万
-
财政年份:1999
-
负责人:FRANCIS W. LUSCINSKAS
-
依托单位:
ENDOTHELIAL CELL DYSFUNCTION IN CHRONIC IMMUNE DISEASES
-
批准号:6080816
-
项目类别:
-
资助金额:$26.7万
-
财政年份:1999
-
负责人:FRANCIS W. LUSCINSKAS
-
依托单位:
ENDOTHELIAL CELL DYSFUNCTION IN CHRONIC IMMUNE DISEASES
-
批准号:6390760
-
项目类别:
-
资助金额:$26.7万
-
财政年份:1999
-
负责人:FRANCIS W. LUSCINSKAS
-
依托单位:
ENDOTHELIAL CELL DYSFUNCTION IN CHRONIC IMMUNE DISEASES
-
批准号:6527578
-
项目类别:
-
资助金额:$26.7万
-
财政年份:1999
-
负责人:FRANCIS W. LUSCINSKAS
-
依托单位:
ENDOTHELIAL LATERAL JUNCTIONS AND LEUKOCYTE TRAFFICKING
-
批准号:6109793
-
项目类别:
-
资助金额:$32.32万
-
财政年份:1999
-
负责人:FRANCIS W. LUSCINSKAS
-
依托单位:
CORE--CELL BIOLOGY SUPPORT
-
批准号:6109799
-
项目类别:
-
资助金额:$32.32万
-
财政年份:1999
-
负责人:FRANCIS W. LUSCINSKAS
-
依托单位:
ENDOTHELIAL CELL DYSFUNCTION IN CHRONIC IMMUNE DISEASES
-
批准号:6185176
-
项目类别:
-
资助金额:$26.7万
-
财政年份:1999
-
负责人:FRANCIS W. LUSCINSKAS
-
依托单位:
ENDOTHELIAL LATERAL JUNCTIONS AND LEUKOCYTE TRAFFICKING
-
批准号:6272750
-
项目类别:
-
资助金额:$31.73万
-
财政年份:1998
-
负责人:FRANCIS W. LUSCINSKAS
-
依托单位:
CORE--CELL BIOLOGY SUPPORT
-
批准号:6272756
-
项目类别:
-
资助金额:$31.73万
-
财政年份:1998
-
负责人:FRANCIS W. LUSCINSKAS
-
依托单位:
海外基金