Cell Biology Support

细胞生物学支持

• 批准号: 7753053
• 负责人: FRANCIS W. LUSCINSKAS
• 金额: $ 21.93万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7753053
• 关键词: Adenoviruses; Adhesives; Animals; Biological; Blood; Blood Vessels; Budgets; Cell Culture System; Cell Culture Techniques; Cell Line; Cells; Cellular biology; Cultured Cells; Decontamination; Dermal; Endothelial Cells; Endothelium; Ensure; Enzymes; Fibroblast Growth Factor; Functional disorder; Funding; Goals; Growth Factor; Human; Human Resources; In Vitro; Individual; Knock-out; Leukocytes; Mediation; Medical; Methodology; Modification; Mus; Price; Procedures; Production; Proteins; Published Comment; Quality Control; Reagent; Recombinants; Research; Research Personnel; Serum; Source; Sterility; Techniques; Testing; Tissues; Training; Transgenic Mice; Umbilical vein; Vascular Endothelial Cell; Work; cost; fetal bovine serum; flasks; graduate student; high standard; human tissue; in vitro Model; neonatal human; neonate; penis foreskin; programs; research study; response; tissue culture; vector

INTRODUCTION TO REVISED CORE A: CELL BIOLOGY SUPPORT: We appreciate the comments provided by reviewers and the fact that both reviewers found that Core A personnel have the expertise to perform the proposed objectives. Thus, the objectives of the Core in this revised application are unchanged. Reviewers raised a concern of overlap in funds available in each of the projects for cell culture, and a concern as to whether the individual projects will have any overlapping function. In response, we have revised the personnel effort and supply budget as suggested, with some modification as explained in the budget justification. In regards to whether projects having overlapping function with Core A, we believe they do not, neither in personnel effort or supple budget. Core A and the personnel in each Project have some expertise in common, but the personnel in Core A alone provide the FTEs that actually isolate, amplify and culture all human and all animal endothelial cells, all cell lines, and other cells used by investigators in the Program, in the all correct plasticware formats for each specific experimental requirement requested by the Project investigators. This provides a critical advantage to the Investigators in the Program because Core personnel will use the highest standards of quality control on all cells, serum and growth factors and all other necessary reagents used in in vitro experiments across the program.

修订核心A：细胞生物学支持简介： 我们感谢评审员提供的评论，以及两位评审员都发现核心A 工作人员具备实现拟议目标的专业知识。因此，核心在这方面的目标 修改后的应用程序不变。审查人员对每个项目的可用资金重叠表示关切， 此外，我们亦关注到，个别项目是否会有任何功能重叠。 作为回应，我们已按照建议修订了人员工作和供应预算，并作了一些修改， 在预算说明中解释。关于项目是否与核心A的职能重叠， 我们认为，无论是在人员努力方面，还是在灵活的预算方面，他们都没有做到这一点。核心A及各项目人员 有一些共同的专业知识，但核心A中的人员单独提供了实际隔离的FTE， 扩增和培养所有人和所有动物内皮细胞、所有细胞系和其他细胞， 研究人员在该计划中，在所有正确的塑料器皿格式为每个特定的实验要求 项目调查人员的要求。这为项目中的研究者提供了关键优势 因为核心人员将对所有细胞、血清和生长因子采用最高标准的质量控制， 以及整个项目中体外实验中使用的所有其他必要试剂。