S PARRYII A MODEL HIBERNATOR PHYSIOLOGY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 拟议研究的目标： 1.校准组织~(15)N的富集量，以便在温度中和(Ta=+2？C)或温度调节(Ta=-10？C)的迟钝的帕里氏葡萄球菌中大量使用。 2.阐明并量化了金黄色葡萄球菌主要代谢基因的表达模式，这些基因既可以是温度中和(+2℃)，也可以是温度调节(-10℃)。 3.描述在极低Ta下冬眠的能量学、底物选择和极限。 4.在自然种群中，冬眠前的身体条件和冬眠温度与身体条件、物候、稳定的同位素特征和成分的越冬变化与适合度之间的关系。 由于它们的新陈代谢和体温具有极强的可塑性，冬眠者已被用作许多人类疾病和伤害的模式物种。 这些疾病包括心脏病、脑缺血、阿尔茨海默氏症和创伤性头部损伤。因此，这项研究的实验室部分的结果可能会对生物医学产生影响。实地研究将大大有助于理解生物对北极气候变化的反应。拟议的工作将用新的跨学科方法解决冬眠生理学中的重要问题。它将为比较生理学做出重大贡献 通过记录极端饥饿动物模型中的组织15N丰度来稳定同位素生态学；以及通过进一步发展RT-PCR方法在生态生理学和生物医学的新模式生物中转录切分来研究比较基因组学。这项工作将把帕里氏锥虫独特的生理特征与冬眠温度的现场测量联系起来，以增加对北极适应的了解，并将提供预测全球气候变化如何影响一个重要北极物种种群动态的能力。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objectives of Proposed Research: 1. Calibrate tissue 15N enrichment to lean mass use in torpid S. parryii that are either thermoneutral (Ta = +2¿C) or thermoregulating (Ta = -10¿C). 2. Elucidate and quantify patterns of expression of key metabolic genes of torpid S. parryii that are either thermoneutral (+2¿C) or thermoregulating (-10¿C). 3. Describe the energetics, substrate selection and limits to hibernation at extreme low Ta. 4. Correlate pre-hibernation body condition and hibernacula temperatures to overwinter changes in body condition, phenology, stable isotope signatures and components to fitness in natural populations. Given the extreme plasticity of their metabolism and body temperature, hibernators have been adopted as a model species for a number of human disease and injury states, including heart disease, ischemia, Alzheimer's, and traumatic head injury. Thus, results from the laboratory portion of this research could have applied implications for biomedicine. Field studies will contribute significantly to understanding organismal responses to a changing arctic climate. Proposed work will address important questions in hibernation physiology with new, interdisciplinary approaches. It will contribute significantly to comparative physiology by establishing limits to hibernation eco-physiology and mechanisms that create these constraints; to stable isotope ecology by documenting tissue 15N enrichment in an extreme animal model of starvation; and to comparative genomics, by further developing the RT-PCR approach to transcriptomics in a new model organism for ecophysiology and biomedicine. The work will link unique physiological traits of S. parryii with field measurements of hibernacula temperatures to increase knowledge of adaptation to the Arctic, and will provide the power to predict how changes in global climate may affect the population dynamics of an important arctic species.