S PARRYII A MODEL HIBERNATOR PHYSIOLOGY

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 拟议研究的目标： 1.校准组织15 N富集以在迟钝S中使用瘦体重。parryii，要么是热中性（Ta = +2 ° C）或温度调节（Ta = -10 ° C）。 2.阐明并量化了迟钝链球菌关键代谢基因的表达模式。parryii，要么热中性（+2 ° C）或温度调节（-10 ° C）。 3.描述能量学、基质选择和在极低Ta下冬眠的限制。 4.将冬眠前的身体状况和冬眠区温度与自然种群中身体状况、物候、稳定同位素特征和组分的越冬变化相关联。 由于冬眠动物的新陈代谢和体温具有极强的可塑性，它们已被用作许多人类疾病和损伤的模式动物 这些疾病包括心脏病、局部缺血、阿尔茨海默氏症和创伤性头部损伤。因此，这项研究的实验室部分的结果可能对生物医学有应用意义。实地研究将大大有助于了解生物对北极气候变化的反应。拟议的工作将解决冬眠生理学的重要问题，新的，跨学科的方法。它将通过建立比较生理学做出重大贡献。 限制冬眠生态生理学和机制，创造这些限制;稳定的同位素生态学，通过记录组织15 N富集在极端动物模型的饥饿;和比较基因组学，通过进一步发展RT-PCR方法，转录组学在一个新的模式生物的生态生理学和生物医学。这项工作将把S. Parryii与冬眠温度的实地测量，以增加适应北极的知识，并将提供预测全球气候变化如何影响一个重要的北极物种的种群动态的力量。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objectives of Proposed Research: 1. Calibrate tissue 15N enrichment to lean mass use in torpid S. parryii that are either thermoneutral (Ta = +2¿C) or thermoregulating (Ta = -10¿C). 2. Elucidate and quantify patterns of expression of key metabolic genes of torpid S. parryii that are either thermoneutral (+2¿C) or thermoregulating (-10¿C). 3. Describe the energetics, substrate selection and limits to hibernation at extreme low Ta. 4. Correlate pre-hibernation body condition and hibernacula temperatures to overwinter changes in body condition, phenology, stable isotope signatures and components to fitness in natural populations. Given the extreme plasticity of their metabolism and body temperature, hibernators have been adopted as a model species for a number of human disease and injury states, including heart disease, ischemia, Alzheimer's, and traumatic head injury. Thus, results from the laboratory portion of this research could have applied implications for biomedicine. Field studies will contribute significantly to understanding organismal responses to a changing arctic climate. Proposed work will address important questions in hibernation physiology with new, interdisciplinary approaches. It will contribute significantly to comparative physiology by establishing limits to hibernation eco-physiology and mechanisms that create these constraints; to stable isotope ecology by documenting tissue 15N enrichment in an extreme animal model of starvation; and to comparative genomics, by further developing the RT-PCR approach to transcriptomics in a new model organism for ecophysiology and biomedicine. The work will link unique physiological traits of S. parryii with field measurements of hibernacula temperatures to increase knowledge of adaptation to the Arctic, and will provide the power to predict how changes in global climate may affect the population dynamics of an important arctic species.