Engineering of cell shape and intracellular organization

细胞形状和细胞内组织工程

基本信息

  • 批准号:
    7849134
  • 负责人:
  • 金额:
    $ 240万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2009
  • 资助国家:
    美国
  • 起止时间:
    2009-09-30 至 2014-06-30
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (Provided by the applicant) Abstract: A bacterial cell is much more than the sum of its parts. Most cellular functions are critically impacted not only by regulation of the genome and proteome, but also by the shape of the cell and how the shape dictates the localization of intracellular components. The ability to systematically manipulate cell shape will ultimately provide a powerful suite of applications in antibiotic drug development, synthetic biology, and biosensing. My laboratory will leverage insight from evolutionary, synthetic, and cell biological approaches to inform our ongoing development of quantitative, biophysical models of bacterial cell shape determination and growth. We have already successfully used modeling to predict the cell shape response to antibiotic treatment. We will focus our efforts on exploiting other predictions generated from quantitative models to reengineer cell shape and redesign the intracellular localization landscape. For the period of this award, three design targets will be pursued that leverage our expertise in biophysical modeling of cell shape to probe key features of cell growth: 1) We will explore the evolutionary origins of cell shape determination by transplanting foreign cytoskeletal elements between closely related bacteria. 2) We will program specific intracellular organizational phenotypes to dynamically reengineer cell shape. 3) We will determine the tension sensitivity of the growth machinery to elucidate potential feedback mechanisms for cell shape maintenance. These targets will strategically expand the experimental focus of my laboratory. Success will address many longstanding questions of how cells determine their shape and how they utilize shape to regulate complex intracellular processes such as cell division. The physical principles of organization are likely to appear in diverse biological contexts, in both bacterial cells and in higher organisms. Ultimately, we will challenge our understanding of cell shape determination by transforming shape into an experimentally tunable parameter. Public Health Relevance: Cell shape and intracellular organization have been recognized to play a critical role in a wide variety of important bacterial functions. We intend to systematically engineer cell shape to elucidate and exploit the links between shape, function, and fitness. Our work will produce tools for altering shape and organization with potential applications in biosensing, control of pathogenesis, and nanotechnology.
描述(由申请人提供) 翻译后摘要:细菌细胞是远远超过其部分的总和。大多数细胞功能不仅受到基因组和蛋白质组调控的严重影响,而且还受到细胞形状以及形状如何决定细胞内组分定位的影响。系统地操纵细胞形状的能力最终将在抗生素药物开发、合成生物学和生物传感方面提供一系列强大的应用。我的实验室将利用进化,合成和细胞生物学方法的见解,为我们正在进行的细菌细胞形状确定和生长的定量生物物理模型的开发提供信息。我们已经成功地使用建模来预测细胞形状对抗生素治疗的反应。我们将集中精力利用定量模型产生的其他预测来重新设计细胞形状和重新设计细胞内定位景观。在此期间,将追求三个设计目标,利用我们在细胞形状生物物理建模方面的专业知识来探索细胞生长的关键特征:1)我们将通过在密切相关的细菌之间移植外源细胞骨架元素来探索细胞形状决定的进化起源。2)我们将对特定的细胞内组织表型进行编程,以动态地重新设计细胞形状。3)我们将确定生长机制的张力敏感性,以阐明细胞形状维持的潜在反馈机制。这些目标将战略性地扩大我实验室的实验重点。成功将解决许多长期存在的问题,即细胞如何决定它们的形状,以及它们如何利用形状来调节复杂的细胞内过程,如细胞分裂。组织的物理原理可能出现在不同的生物环境中,在细菌细胞和高等生物中。最终,我们将挑战我们的理解细胞形状的决定,通过将形状转化为一个实验可调参数。 公共卫生相关性:细胞形状和细胞内组织已被认为在各种重要的细菌功能中发挥关键作用。我们打算系统地设计细胞形状,以阐明和利用形状,功能和健身之间的联系。我们的工作将产生改变形状和组织的工具,在生物传感,控制发病机制和纳米技术中具有潜在的应用。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

KERWYN C. HUANG其他文献

KERWYN C. HUANG的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('KERWYN C. HUANG', 18)}}的其他基金

Molecular Biophysics Training Program at Stanford
斯坦福大学分子生物物理学培训项目
  • 批准号:
    10647893
  • 财政年份:
    2021
  • 资助金额:
    $ 240万
  • 项目类别:
Molecular Biophysics Training Program at Stanford
斯坦福大学分子生物物理学培训项目
  • 批准号:
    10207153
  • 财政年份:
    2021
  • 资助金额:
    $ 240万
  • 项目类别:
Molecular Biophysics Training Program at Stanford
斯坦福大学分子生物物理学培训项目
  • 批准号:
    10434837
  • 财政年份:
    2021
  • 资助金额:
    $ 240万
  • 项目类别:
A Molecular Study of Min-Protein Polymer Dynamics
最小蛋白聚合物动力学的分子研究
  • 批准号:
    7478728
  • 财政年份:
    2005
  • 资助金额:
    $ 240万
  • 项目类别:
A Molecular Study of Min-Protein Polymer Dynamics
最小蛋白聚合物动力学的分子研究
  • 批准号:
    7684817
  • 财政年份:
    2005
  • 资助金额:
    $ 240万
  • 项目类别:
A Molecular Study of Min-Protein Polymer Dynamics
最小蛋白聚合物动力学的分子研究
  • 批准号:
    7271896
  • 财政年份:
    2005
  • 资助金额:
    $ 240万
  • 项目类别:
Molecular Study of Min-Protein Polymer Dynamics
最小蛋白聚合物动力学的分子研究
  • 批准号:
    6957517
  • 财政年份:
    2005
  • 资助金额:
    $ 240万
  • 项目类别:
A Molecular Study of Min-Protein Polymer Dynamics
最小蛋白聚合物动力学的分子研究
  • 批准号:
    7097278
  • 财政年份:
    2005
  • 资助金额:
    $ 240万
  • 项目类别:

相似海外基金

SBIR Phase II: Development of a urine dipstick test that can guide immediate and appropriate antibiotic therapy for treatment of complicated urinary tract infections
SBIR II 期:开发尿液试纸测试,可以指导复杂尿路感染的立即和适当的抗生素治疗
  • 批准号:
    2213034
  • 财政年份:
    2023
  • 资助金额:
    $ 240万
  • 项目类别:
    Cooperative Agreement
Personalized Antibiotic Therapy in the Emergency Department: PANTHER Trial
急诊科的个性化抗生素治疗:PANTHER 试验
  • 批准号:
    10645528
  • 财政年份:
    2023
  • 资助金额:
    $ 240万
  • 项目类别:
Strategies for improving the efficacy of combinatorial antibiotic therapy in chronic infections
提高慢性感染联合抗生素治疗疗效的策略
  • 批准号:
    10736285
  • 财政年份:
    2023
  • 资助金额:
    $ 240万
  • 项目类别:
A Novel Bone Targeted Antibiotic Therapy for the Treatment of Infected Fractures
一种治疗感染性骨折的新型骨靶向抗生素疗法
  • 批准号:
    10603486
  • 财政年份:
    2023
  • 资助金额:
    $ 240万
  • 项目类别:
Severe Cutaneous Adverse Reactions Following Outpatient Antibiotic Therapy: A Population-based Study
门诊抗生素治疗后的严重皮肤不良反应:一项基于人群的研究
  • 批准号:
    449379
  • 财政年份:
    2020
  • 资助金额:
    $ 240万
  • 项目类别:
    Studentship Programs
Sex-Specific Differences in End-of-Life Burdensome Interventions and Antibiotic Therapy in Nursing Home Residents With Advanced Dementia
患有晚期痴呆症的疗养院居民的临终干预和抗生素治疗的性别差异
  • 批准号:
    422034
  • 财政年份:
    2020
  • 资助金额:
    $ 240万
  • 项目类别:
Optimizing outpatient parenteral antibiotic therapy to support hospital-in-the-home program across the unique environmental conditions of Australia
优化门诊肠外抗生素治疗,以支持澳大利亚独特环境条件下的家庭医院计划
  • 批准号:
    nhmrc : 1197866
  • 财政年份:
    2020
  • 资助金额:
    $ 240万
  • 项目类别:
    Investigator Grants
Resistance evolution in the presence of combination antibiotic therapy
联合抗生素治疗下耐药性的演变
  • 批准号:
    2241853
  • 财政年份:
    2019
  • 资助金额:
    $ 240万
  • 项目类别:
    Studentship
Host-pathogen interactions in antibiotic therapy for listeriosis
李斯特菌病抗生素治疗中宿主与病原体的相互作用
  • 批准号:
    18K07106
  • 财政年份:
    2018
  • 资助金额:
    $ 240万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Multipurpose targeted nano-antibiotic therapy to fight tough infection in bones
多用途靶向纳米抗生素疗法可对抗骨骼中的严重感染
  • 批准号:
    9788269
  • 财政年份:
    2018
  • 资助金额:
    $ 240万
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了