Group Training in Product Development and Manufacturing

专性细胞内病原体的繁殖和定量是劳动密集型和昂贵的 事业的这些特性，再加上C.伯内特氏菌高度传染性和选择性病原体 命名，使最基本的研究工作复杂化。然而，由于高纯度和 枚举C.伯内特是必不可少的拟议研究，一个专用的核心设施与BSL 3 遏制，训练有素的技术人员，并提出了一个档案系统。因此， 核心B是向蒙大拿州4个柯克斯体项目中的每一个提供种植方面的专业知识， 1期和2期贝氏柯克斯体的纯化、计数和存档。核心将位于 蒙大拿州立大学，BSL-3设施的位置，并将由艾伦哈姆森的 蒙大拿州立大学。 项目之间的合作。核心项目B将分别为 Montana Coxiella组以及任何其他需要他们服务的RMRCE研究者。这些服务将 包括制作贝氏柯克斯体1期和2期的标准化接种物以及 Coxiella，以及Coxiella生物体的标准化计数和存档服务。在 此外，这两名兼职技术人员还接受过专门的培训，在BSL-3中对小鼠进行肺部感染。 在不从事其他活动时，核心B技术人员将协助蒙大拿州4人 Coxiella预测它们牺牲小鼠的日子，因为这些时间点是劳动密集型的。我们的BSL 3 SOP 需要一个“伙伴系统”，个人不允许单独在设施中工作。得多 经济有两个兼职浮动职位，而不是为每个单独的研究项目， 为全职技术人员提供资金，以覆盖这些偶尔的劳动密集型时间点。因此，核心是 对每个项目的目标是不可或缺的，是项目之间保持一致性所必需的， 项目之间的结果可以直接比较。 核心B将支持RMRCE免疫调节、佐剂和疫苗综合研究重点 (IRF 1）以及细菌治疗学（IRF 2），其资源将由RP 1.1、1.2、1.3和 2.4.

Propagation and quantification of obligate intracellular pathogens are both labor-intensive and costly undertakings. These characteristics, coupled with C. burnetii's highly infectious nature and select agent designation, complicate the most basic research endeavors. However, because highly purified and enumerated C. burnetii is essential to the proposed research, a dedicated core facility with BSL 3 containment, highly trained technical personnel, and an archival system is proposed. Thus, the purpose of the Core B is to make available to each of the 4 Montana Coxiella projects expertise in the cultivation, purification, enumeration, and archiving of phase 1 and 2 Coxiella burnetii. The core will be located at Montana State University, the location of the BSL-3 facilities, and will be directed by Allen Harmsen of Montana State University. Collaborations among projects. Core B will provide services to each of the 4 research projects of the Montana Coxiella group as well as any other RMRCE investigator needing their service. These services will include making standardized inocula of phase 1 and 2 Coxiella burnetii as well as the Crazy strain of Coxiella, as well as the services of standardized Coxiella enumeration and archival of Coxiella organisms. In addition, the two part- time technicians are trained specifically in doing lung infections in mice in the BSL-3. When not engaged in their other activities, the Core B technicians will be available to assist the 4 Montana Coxiella projects on days they sacrifice mice since these time points are labor intensive. Our BSL3 SOP requires a "buddy system" and individuals are not allowed to work alone in the facility. It is much more economical to have two part time floating positions rather than for each of the individual research projects to fund full time technicians to cover these occasional, labor-intensive time points. Therefore, the core is integral and critical to the goals of each of the projects and required for consistency between projects so that results between projects can be directly compared. Core B will support the RMRCE Integrated Research Foci on Immunomodulation, Adjuvants and Vaccines (IRF 1) as well as Bacterial Therapeutics (IRF 2), and its resources will be utilized by RPs 1.1, 1.2, 1.3, and 2.4.