Role of the STK Receptor in Erythropoiesis
STK 受体在红细胞生成中的作用
基本信息
- 批准号:7819133
- 负责人:
- 金额:$ 1.6万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2009
- 资助国家:美国
- 起止时间:2009-06-01 至 2010-08-31
- 项目状态:已结题
- 来源:
- 关键词:AcuteAcute Erythroblastic LeukemiaAnemiaBindingBinding SitesBiochemicalBiological ModelsBone MarrowBone Marrow CellsCellsComplexDataDevelopmentDiagnostic Neoplasm StagingDiseaseDisease ProgressionDockingErythroblastsErythroid Progenitor CellsErythropoiesisEventFamily memberFriend Murine Leukemia VirusFriendsGene TargetingGenesGeneticGenetic TranscriptionGenomeGlycoproteinsGrantGrowthIn VitroInfectionLeadMediatingModelingMusMutationPECAM1 genePathway interactionsPhasePhosphotransferasesProcessProtein Tyrosine KinaseRadiationRadioprotectionReceptor Protein-Tyrosine KinasesRegulationResistanceRetroviridaeRoleSignal PathwaySignal TransductionSiteSpleenSpleen Focus-Forming VirusesStagingStem cellsStressStudy modelsSystemTP53 geneTailTestingTyrosineUp-RegulationViralVirusVirus DiseasesVirus Replicationbiological adaptation to stresscarcinogenesiscytokinedomain mappinghuman MST1R proteinin vivoinsightleukemiamutantprogenitorreceptorreconstitutionresponsesealsrc-Family Kinasestherapeutic target
项目摘要
DESCRIPTION (provided by applicant): Friend leukemia virus provides an ideal model system for studying the multistage progression of carcinogenesis. It is composed of two viruses, the spleen focus forming virus (SFFV) and the replication competent F-MuLV. In the early phase of disease, a viral glycoprotein, gp55. encoded by SFFV interacts with and causes constitutive activation of a truncated form of the STK receptor tyrosine kinase (Sf-Stk) and the EpoR. These signals drive a polyclonal expansion of infected cells in the spleen. Mutations in p53 and integration of F-MuLV in the host genome resulting in increased transcription of the ets family members PU.1 and Fli-1, lead to leukemic transformation in the late stages of the disease. We set out to determine the mechanism by which Sf-Stk induces the early stages of transformation in this model system. To do this, we have developed an in vitro system in which primary bone marrow cells lacking Sf-Stk can be reconstituted with wild-type and mutant forms of the receptor. The cells are then infected with Friend virus and the ability of gp55 to induce cytokine-independent growth of the progenitors is assessed. Using this approach, we have shown that the kinase activity of Sf-Stk and the Grb2 binding site are critical for transformation. We have extended those studies using mice with targeted deletions in Grb2 and Gab2, that a Grb2/Gab2 complex is required downstream of Sf-Stk, leading to the recruitment and activation of Stat3. In this proposal we will identify the target cells of Friend virus in the bone marrow and spleen and investigate the role of Sf- Stk in the regulation of these cells in response to radiation or acute anemia. We propose that Friend virus co-opts normal stress response pathways to induce the rapid polyclonal expansion of infected progenitor cells. Further, we will study the role of Gab2 and Stat3 in the process of transformation of primary erythroblasts by Friend virus. Towards that end, we will utilize both genetic and biochemical approaches to map domains of Gab2 and Stat3 required for this response. We propose that this signaling pathway leads to the upregulation of PU.1 prior to retroviral insertion, resulting in the inhibition of differentiation. Taken together, these data will provide new insight into the early stages of leukemic transformation as well as the potential parallels between Friend virus-induced erythropoietic expansion and mechanisms involved in the response of these cells to stress, and provide new information regarding potential therapeutic targets.
描述(由申请人提供):Friend白血病病毒为研究致癌作用的多阶段进展提供了理想的模型系统。它由两种病毒组成,即脾病灶形成病毒(SFFV)和具有复制能力的F-MuLV。在疾病的早期阶段,一种病毒糖蛋白gp 55。由SFFV编码的EpoR与截短形式的STK受体酪氨酸激酶(Sf-Stk)和EpoR相互作用并引起其组成性激活。这些信号驱动脾脏中受感染细胞的多克隆扩增。p53突变和宿主基因组中F-MuLV的整合导致ets家族成员PU.1和Fli-1的转录增加,导致疾病晚期的白血病转化。我们着手确定Sf-Stk在该模型系统中诱导转化早期阶段的机制。为此,我们开发了一种体外系统,其中缺乏Sf-Stk的原代骨髓细胞可以用野生型和突变型受体重建。然后用Friend病毒感染细胞,并评估gp 55诱导祖细胞的非依赖于嘌呤的生长的能力。使用这种方法,我们已经表明,Sf-Stk的激酶活性和Grb 2结合位点是转化的关键。我们已经扩展了这些研究,使用Grb 2和Gab 2靶向缺失的小鼠,即Sf-Stk下游需要Grb 2/Gab 2复合物,导致Stat 3的募集和激活。在这个提议中,我们将确定Friend病毒在骨髓和脾脏中的靶细胞,并研究Sf-Stk在这些细胞对辐射或急性贫血反应的调节中的作用。我们认为Friend病毒通过选择正常的应激反应途径来诱导受感染祖细胞的快速多克隆扩增。进一步,我们将研究Gab 2和Stat 3在Friend病毒转化原代成红细胞过程中的作用。为此,我们将利用遗传和生化方法来映射这种反应所需的Gab 2和Stat 3结构域。我们提出,这种信号通路导致逆转录病毒插入前PU.1的上调,从而抑制分化。总之,这些数据将提供新的见解白血病转化的早期阶段,以及朋友病毒诱导的红细胞生成扩增和机制参与这些细胞的反应,以压力之间的潜在相似之处,并提供新的信息,潜在的治疗目标。
项目成果
期刊论文数量(7)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Uncoupling ligand-dependent and -independent mechanisms for mitogen-activated protein kinase activation by the murine Ron receptor tyrosine kinase.
鼠 Ron 受体酪氨酸激酶解偶联丝裂原激活蛋白激酶激活的配体依赖性和非依赖性机制。
- DOI:10.1074/jbc.m505737200
- 发表时间:2005
- 期刊:
- 影响因子:0
- 作者:Wei,Xin;Ni,Shuang;Correll,PamelaH
- 通讯作者:Correll,PamelaH
Resistance to friend virus-induced erythroleukemia in W/W(v) mice is caused by a spleen-specific defect which results in a severe reduction in target cells and a lack of Sf-Stk expression.
W/W(v) 小鼠对友人病毒诱导的红白血病的抵抗力是由脾脏特异性缺陷引起的,该缺陷导致靶细胞严重减少和 Sf-Stk 表达缺乏。
- DOI:10.1128/jvi.79.23.14586-14594.2005
- 发表时间:2005
- 期刊:
- 影响因子:5.4
- 作者:Subramanian,Aparna;Teal,HamiE;Correll,PamelaH;Paulson,RobertF
- 通讯作者:Paulson,RobertF
Mutation of the Lyn tyrosine kinase delays the progression of Friend virus induced erythroleukemia without affecting susceptibility.
Lyn 酪氨酸激酶的突变可延缓弗兰德病毒诱导的红白血病的进展,而不影响易感性。
- DOI:10.1016/j.leukres.2006.02.006
- 发表时间:2006
- 期刊:
- 影响因子:2.7
- 作者:Subramanian,Aparna;Hegde,Shailaja;Correll,PamelaH;Paulson,RobertF
- 通讯作者:Paulson,RobertF
Friend virus utilizes the BMP4-dependent stress erythropoiesis pathway to induce erythroleukemia.
Friend病毒利用BMP4依赖性应激红细胞生成途径诱发红白血病。
- DOI:10.1128/jvi.02487-06
- 发表时间:2008
- 期刊:
- 影响因子:5.4
- 作者:Subramanian,Aparna;Hegde,Shailaja;Porayette,Prashanth;Yon,Michele;Hankey,Pamela;Paulson,RobertF
- 通讯作者:Paulson,RobertF
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Pamela A Giblin其他文献
Pamela A Giblin的其他文献
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{{ truncateString('Pamela A Giblin', 18)}}的其他基金
Role of Gab1 and Gab2 in Stress Erythropoiesis
Gab1 和 Gab2 在应激性红细胞生成中的作用
- 批准号:
8439536 - 财政年份:2012
- 资助金额:
$ 1.6万 - 项目类别:
Alcorn State University: Penn State University Bridges to the Doctorate Program
奥尔康州立大学:宾夕法尼亚州立大学通往博士学位课程的桥梁
- 批准号:
8137905 - 财政年份:2006
- 资助金额:
$ 1.6万 - 项目类别:
Alcorn State University: Penn State University Bridges to the Doctorate Program
奥尔康州立大学:宾夕法尼亚州立大学通往博士学位课程的桥梁
- 批准号:
8324545 - 财政年份:2006
- 资助金额:
$ 1.6万 - 项目类别:
Alcorn State University: Penn State University Bridges to the Doctorate Program
奥尔康州立大学:宾夕法尼亚州立大学通往博士学位课程的桥梁
- 批准号:
8543743 - 财政年份:2006
- 资助金额:
$ 1.6万 - 项目类别:
Alcorn State University: Penn State University Bridges to the Doctorate Program
奥尔康州立大学:宾夕法尼亚州立大学通往博士学位课程的桥梁
- 批准号:
7936470 - 财政年份:2006
- 资助金额:
$ 1.6万 - 项目类别: