BIOMOLECULE ANALYSIS CORE FACILITY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们建议继续支持我们专门从事生物分子分析的多用户研究机构--生物分子分析核心机构(BACF)。BACF拥有尖端设备，如质谱仪、NanoLC系统、表面等离子激元共振(Biacore)、高效液相系统、分光光度计、荧光计和发光计、超速离心机、制备离心机和其他几种共享仪器。还有许多其他资源可用于记录和分析使用这些仪器收集的实验数据。该核心的长期目标是为最广泛的用户群体提供用于生物分子分析的维护良好的尖端设备。该设施现在位于新的生物科学大楼，面积为1650平方英尺。BACF目前由7个UTEP部门和埃尔帕索的休斯顿公共卫生学院使用，代表着50个研究小组、40名教职员工、18名研究助理(工作人员)、16名博士后、48名博士生、32名硕士研究生和128名本科生。核心目前平均每月访问1,000次(约每年12,000个)，由249个个人用户使用。核心由一名全职技术人员(协调员)组成，负责工厂技术人员和工程师的日常维护计划、预防性维护、用户导向和协助，以及账户和库存维护。将向负责监督这些活动的核心主管提供部分薪金。为了实现设备的自给自足维护，我们计划向UTEP/BBRC和外部用户提供新的核心服务，如肽测序/LC-MS/MS蛋白质组分析。为此，我们在这里申请资金，聘请一名博士级别的全职研究助理或技术人员来监督质谱学和蛋白质组分析活动。为了成功实现我们的目标，提出了三个具体目标：具体目标1：继续为BBRC研究人员和外部用户提供维护良好的最先进设备。具体目标2：继续在核心设备的使用方面提供技术指导支持。具体目标#3：提供旨在自给自足维护核心的服务。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We propose continuing support for our multi-user research facility specializing in the analysis of biomolecules, the Biomolecule Analysis Core Facility (BACF). The BACF houses cutting-edge equipment, such as mass spectrometers, nanoLC systems, surface plasmon resonance (BIACORE), HPLC systems, spectrophotometers, fluorometer and luminometer, ultracentrifuges, preparative centrifuges, and several other shared instruments. Numerous other resources are available for documentation and analysis of experimental data collected using these instruments. The long-term objective of this Core is to provide the broadest possible group of users with access to well-maintained, cutting-edge equipment for biomolecule analysis. The facility is now housed in the new Bioscience Building, in an area of 1,650 sq feet. The BACF is currently used by 7 UTEP departments and the School of Public Health Houston at El Paso, representing 50 research groups, 40 faculty members, 18 research assistants (staff), 16 post-docs, 48 PhD students, 32 MS students, and 128 undergraduate students. The Core is currently accessed in average 1,000 times per month (approx. 12,000 per year), by 249 individual users. The Core is staffed by a full-time technician (coordinator), who is responsible for routine maintenance scheduling preventive maintenance by factory technicians and engineers, user orientation and assistance, as well as account and inventory maintenance. Partial salary will be provided to the core director for overseeing these activities. Aiming at a self-sustaining maintenance of the facility, we plan to offer new core services, such as peptide sequencing/proteomic analysis by LC-MS/MS, to UTEP/BBRC and external users. For this, here we request funds for hiring one full-time research associate or technician, at PhD level, to oversee the mass spectrometry and proteomic analysis activities. To successfully achieve our objectives, three specific aims are proposed: Specific Aim #1: Continue to provide BBRC researchers and external users access to well-maintained, state-of-the-art equipment. Specific Aim #2: Continue to provide technical instructional support in the use of Core equipment. Specific Aim # 3: To provide services aiming at a self-sustaining maintenance of the Core.