BIOMOLECULE ANALYSIS CORE FACILITY

生物分子分析核心设施

• 批准号: 8166186
• 负责人: IGOR C ALMEIDA
• 金额: $ 22.49万
• 依托单位: UNIVERSITY OF TEXAS EL PASO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8166186
• 关键词: Accounting; Area; Arts; Computer Retrieval of Information on Scientific Projects Database; Core Facility; Data; Doctor of Philosophy; Documentation; Engineering; Equipment; Equipment and supply inventories; Faculty; Funding; Grant; High Pressure Liquid Chromatography; Housing; Individual; Institution; Maintenance; Mass Spectrum Analysis; Postdoctoral Fellow; Preventive; Proteomics; Public Health Schools; Research; Research Personnel; Resources; Schedule; Services; Source; Students; Surface Plasmon Resonance; System; Time; United States National Institutes of Health; Wages; experimental analysis; foot; instrument; mass spectrometer; member; protein aminoacid sequence; research facility

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We propose continuing support for our multi-user research facility specializing in the analysis of biomolecules, the Biomolecule Analysis Core Facility (BACF). The BACF houses cutting-edge equipment, such as mass spectrometers, nanoLC systems, surface plasmon resonance (BIACORE), HPLC systems, spectrophotometers, fluorometer and luminometer, ultracentrifuges, preparative centrifuges, and several other shared instruments. Numerous other resources are available for documentation and analysis of experimental data collected using these instruments. The long-term objective of this Core is to provide the broadest possible group of users with access to well-maintained, cutting-edge equipment for biomolecule analysis. The facility is now housed in the new Bioscience Building, in an area of 1,650 sq feet. The BACF is currently used by 7 UTEP departments and the School of Public Health Houston at El Paso, representing 50 research groups, 40 faculty members, 18 research assistants (staff), 16 post-docs, 48 PhD students, 32 MS students, and 128 undergraduate students. The Core is currently accessed in average 1,000 times per month (approx. 12,000 per year), by 249 individual users. The Core is staffed by a full-time technician (coordinator), who is responsible for routine maintenance scheduling preventive maintenance by factory technicians and engineers, user orientation and assistance, as well as account and inventory maintenance. Partial salary will be provided to the core director for overseeing these activities. Aiming at a self-sustaining maintenance of the facility, we plan to offer new core services, such as peptide sequencing/proteomic analysis by LC-MS/MS, to UTEP/BBRC and external users. For this, here we request funds for hiring one full-time research associate or technician, at PhD level, to oversee the mass spectrometry and proteomic analysis activities. To successfully achieve our objectives, three specific aims are proposed: Specific Aim #1: Continue to provide BBRC researchers and external users access to well-maintained, state-of-the-art equipment. Specific Aim #2: Continue to provide technical instructional support in the use of Core equipment. Specific Aim # 3: To provide services aiming at a self-sustaining maintenance of the Core.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 我们建议继续支持我们专门从事生物分子分析的多用户研究设施，即生物分子分析核心设施（BACF）。 BACF 拥有尖端设备，如质谱仪、nanoLC 系统、表面等离子共振 (BIACORE)、HPLC 系统、分光光度计、荧光计和发光计、超速离心机、制备型离心机和其他一些共享仪器。许多其他资源可用于记录和分析使用这些仪器收集的实验数据。该核心的长期目标是为尽可能广泛的用户群体提供维护良好的尖端设备进行生物分子分析。该设施现位于新的生物科学大楼，面积为 1,650 平方英尺。 BACF 目前由 UTEP 的 7 个院系和埃尔帕索休斯顿公共卫生学院使用，代表 50 个研究小组、40 名教员、18 名研究助理（工作人员）、16 名博士后、48 名博士生、32 名硕士生和 128 名本科生。目前，Core 的平均访问量为 1,000 次（每年约 12,000 次），有 249 名个人用户。核心人员配备一名全职技术人员（协调员），负责工厂技术人员和工程师的日常维护安排、预防性维护、用户指导和帮助以及账户和库存维护。将向负责监督这些活动的核心董事提供部分工资。为了实现设施的自我维持维护，我们计划向 UTEP/BBRC 和外部用户提供新的核心服务，例如通过 LC-MS/MS 进行肽测序/蛋白质组分析。为此，我们在此请求资金聘请一名全职博士级研究员或技术人员来监督质谱和蛋白质组分析活动。为了成功实现我们的目标，提出了三个具体目标： 具体目标#1：继续为 BBRC 研究人员和外部用户提供维护良好、最先进的设备。具体目标#2：继续提供核心设备使用方面的技术指导支持。具体目标#3：提供旨在实现核心自我维持的服务。