QUENCHING OF HBAP-CN PEPTIDE FLUORESCENCE BY HALOTHANE

氟烷猝灭 HBAP-CN 肽荧光

• 批准号: 8169548
• 负责人: J KENT BLASIE
• 金额: $ 0.42万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169548
• 关键词: Binding; Binding Sites; Buffers; Computer Retrieval of Information on Scientific Projects Database; Detergents; Fluorescence; Funding; General anesthetic drugs; Goals; Grant; Halothane; Institution; Lasers; Measurement; Measures; Peptides; Phenylalanine; Research; Research Personnel; Resources; Sapphire; Solutions; Source; United States National Institutes of Health; aqueous; fluorophore

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. hbAP-CN is a halothane binding amphiphilic peptide which forms a 4-helix bundle in aqueous detergent buffer solution. Halothane, an inhalational general anesthetic, binds to the cavity in the hydrophobic core of the bundle. A fluorophore incorporated into each peptide helical strand, cyano-phenylalanine (Phe-CN) is located right next to the halothane binding site. Halothane is known to quench fluorescence when it binds to hbAP-CN. The goal of doing a fluorescence study including the measurement of fluorescent spetra and lifetimes as a function of halothane concentration is to better understand the binding site and quenching mechanism (static or dynamic). It it planned to measure fluorescence lifetimes at the Resource using 270nm excitation (tripled Ti:sapphire laser) of hbAP-CN and Phe-CN itself.

这个子项目是众多研究子项目之一