QUENCHING OF HBAP-CN PEPTIDE FLUORESCENCE BY HALOTHANE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 HBAP-CN是一种与氟烷结合的两亲性多肽，在洗涤剂缓冲溶液中形成4-螺旋束。氟烷是一种吸入性全身麻醉剂，与管束疏水核心中的空腔结合。氰基苯丙氨酸(Phe-CN)是一个结合在每个多肽螺旋链上的荧光团，位于氟烷结合部位的旁边。已知氟烷在与hbAP-CN结合时可以猝灭荧光。进行荧光研究，包括测量荧光光谱和寿命随氟烷浓度的变化，目的是更好地了解结合位置和猝灭机制(静态或动态)。它计划使用270 nm的hbAP-CN和Phe-CN本身的激发(三倍钛宝石激光)测量资源的荧光寿命。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. hbAP-CN is a halothane binding amphiphilic peptide which forms a 4-helix bundle in aqueous detergent buffer solution. Halothane, an inhalational general anesthetic, binds to the cavity in the hydrophobic core of the bundle. A fluorophore incorporated into each peptide helical strand, cyano-phenylalanine (Phe-CN) is located right next to the halothane binding site. Halothane is known to quench fluorescence when it binds to hbAP-CN. The goal of doing a fluorescence study including the measurement of fluorescent spetra and lifetimes as a function of halothane concentration is to better understand the binding site and quenching mechanism (static or dynamic). It it planned to measure fluorescence lifetimes at the Resource using 270nm excitation (tripled Ti:sapphire laser) of hbAP-CN and Phe-CN itself.